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Cornea and External Eye Disease pp 173–208Cite as

In Vivo Micromorphology of the Cornea: Confocal Microscopy Principles and Clinical Applications

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References

  1. Ardjomand N, Komericki P, Radner H, Aigner R, Reich ME (1997) Korneale Langerhanszellen — Verhalten während der Lagerung in Organkultur. Ophthalmologe 94:703–706

    CAS  PubMed  Google Scholar 

  2. Auran JD, Starr MB, Koester CJ, LaBombardi VJ (1994) In vivo scanning slit confocal microscopy of Acanthamoeba keratitis. A case report. Cornea 13:183–185

    CAS  PubMed  Google Scholar 

  3. Banchereau J, Steinman RM (1998) Dendritic cells and the control of the immunity. Nature 392: 245–252

    Article  CAS  PubMed  Google Scholar 

  4. Berlau J, Becker HH, Stave J, Oriwol C, Guthoff RF (2002) Depth and age-dependent distribution of keratocytes in healthy human corneas: a study using scanning-slit confocal microscopy in vivo. J Cataract Refract Surg 28:611–616

    Article  PubMed  Google Scholar 

  5. Bigar F, Thaer A (1994) Untersuchung der Hornhaut intakter Spenderaugen mit konfokalem Spalt-Scanning Video-Mikroskop. Klin Monatsbl Augenheilkd 204:421–423

    CAS  PubMed  Google Scholar 

  6. Böhnke M, Thaer AA (1994) Untersuchung der Kornea mit einem neuen konfokalen Mikroskop. In: Lund OE, Waubke TN (eds) Bildgebende Verfahren in der Augenheilkunde. Methoden und Indikationen. Enke, Stuttgart, pp 47–53

    Google Scholar 

  7. Böhnke M, Masters BR (1999) Confocal microscopy of the cornea. Prog Retin Eye Res 18:553–628

    Article  PubMed  Google Scholar 

  8. Bourne WM, Kaufman HE (1976) Specular microscopy of human corneal endothelium in vivo. Am J Ophthalmol 81:319–323

    CAS  PubMed  Google Scholar 

  9. Cavanagh HD, Jester JV, Essepian J, Shields W, Lemp MA (1990) Confocal microscopy of the living eye. CLAO J 16:65–73

    CAS  PubMed  Google Scholar 

  10. Cavanagh DH, Petroll WM, Alizadeh H, He YG, McCulley JP, Jester JV (1993) Clinical and diagnostic use of in vivo confocal microscopy in patients with corneal disease. Ophthalmology 100:1444–1454

    CAS  PubMed  Google Scholar 

  11. Coleman CM, Hannush S, Covello SP, Smith FJ, Uitto J, McLean WH (1999) A novel mutation in the helix termination motif of keratin K12 in a US family with Meesmann corneal dystrophy. Am J Ophthalmol 128:687–691

    Article  CAS  PubMed  Google Scholar 

  12. Dua HS, Azuara-Blanco A (2000) Limbal stem cells of the corneal epithelium. Surv Ophthalmol 44:415–425

    CAS  PubMed  Google Scholar 

  13. Duke-Elder S, Wybar KC (eds) (1958) System of ophthalmology, vol II. Henry Kimpton, London, pp 113–127; 543–557

    Google Scholar 

  14. Dunn A, Smithpeter C, Welch AJ, Richards-Kortum R (1997) Finite-difference time-domain simulation of light scattering from single cells. J Biomed Opt 2:262–266

    Google Scholar 

  15. Fagerholm P (2000) Wound healing after photorefractive keratectomy. J Cataract Refract Surg 26:432–447

    Article  CAS  PubMed  Google Scholar 

  16. Feenstra RP, Tseng SC (1992) Comparison of fluorescein and rose bengal staining. Ophthalmology 99:605–617

    CAS  PubMed  Google Scholar 

  17. Fromm, Groenouw (1891) Ueber die diagnostische Verwendbarkeit der Flouresceïnfärbung bei Augenerkrankungen. Arch Augenheilkd 22:247–257

    Google Scholar 

  18. Gillette TE, Chandler JW, Greiner JV (1982) Langerhans cells of the ocular surface. Ophthalmology 89:700–711

    CAS  PubMed  Google Scholar 

  19. Gullstrand A (1930) Demonstration der Nernstspaltlampe. Verslg ophthalm Ges Heidelberg 1911, 374, cited in: Vogt A (ed) Lehrbuch und Atlas der Spaltlampenmikroskopie des lebenden Auges, 2nd edn. Verlag von Julius Springer, Berlin, p 1

    Google Scholar 

  20. Hahnel C, Somodi S, Weiss DG, Guthoff RF (2000) The keratocyte network of human cornea: a three-dimensional study using confocal laser scanning fluorescence microscopy. Cornea 19:185–193

    Article  CAS  PubMed  Google Scholar 

  21. Hamrah P, Huq SO, Liu Y, Zhang Q, Dana MR (2003) Corneal immunity is mediated by heterogeneous population of antigen-presenting cells. J Leukoc Biol 74:172–178

    Article  CAS  PubMed  Google Scholar 

  22. Hara M, Morishige N, Chikama T, Nishida T (2003) Comparison of confocal biomicroscopy and noncontact specular microscopy for evaluation of the corneal endothelium. Cornea 22:512–515

    Article  PubMed  Google Scholar 

  23. Harrison DA, Joos C, Ambrosio R (2003) Morphology of corneal basal epithelial cells by in vivo slit-scanning confocal microscopy. Cornea 22: 246–248

    Article  PubMed  Google Scholar 

  24. Holopainen JM, Moilanen JA, Tervo TM (2003) In vivo confocal microscopy of Fleck dystrophy and pre-Descemet’s membrane corneal dystrophy. Cornea 22:160–163

    Article  PubMed  Google Scholar 

  25. Ivarsen A, Laurberg T, Moller-Pedersen T (2004) Role of keratocyte loss on corneal wound repair after LASIK. Invest Ophthalmol Vis Sci 45:3499–3506

    Article  PubMed  Google Scholar 

  26. Kaufmann T, Bodanowitz S, Hesse L, Kroll P (1997) Corneal reinnervation after photorefractive keratectomy and laser in situ keratomileusis and in-vivo study with a confocal video microscope. German J Ophthalmol 5:508–512

    Google Scholar 

  27. Klintworth GK (2003) The molecular genetics of the corneal dystrophies — current status. Front Biosci 8:d687–713

    CAS  PubMed  Google Scholar 

  28. Koester CJ, Roberts CW, Donn A, Hoefle FB (1980) Wide field specular microscopy. Clinical and research applications. Ophthalmology 87:849–860

    CAS  PubMed  Google Scholar 

  29. Kraak R, Stave J, Guthoff RF (2004) In vivo Untersuchung des Verteilungsmusters von Na-Fluorescein an menschlichen Hornhäuten mittels konfokaler Laser-Scanning Fluoreszenzmikroskopie. Paper presented at the 102nd Meeting of the Deutsche Ophthalmologische Gesellschaft, Berlin

    Google Scholar 

  30. Laibson PR, Dhiri S, Oconer J, Ortolan G (1970) Corneal infiltrates in epidemic keratoconjunctivitis. Response to double-blind corticosteroid therapy. Arch Ophthalmol 84:36–40

    CAS  PubMed  Google Scholar 

  31. Laing RA, Sandstrom MM, Leibowitz HM (1975) In vivo photomicrography of the corneal endothelium. Arch Ophthalmol 93:143–145

    CAS  PubMed  Google Scholar 

  32. Li HF, Petroll WM, Moller-Pedersen T, Maurer JK, Cavanagh HD, Jester JV (1997) Epithelial and corneal thickness measurements by in vivo confocal microscopy through focusing (CMTF). Curr Eye Res 16:214–221

    Article  CAS  PubMed  Google Scholar 

  33. Lund O-E, Stefani FH (1978) Corneal histology after epidemic keratoconjunctivitis. Arch Ophthalmol 96:2085–2088

    CAS  PubMed  Google Scholar 

  34. Masters BR, Thaer AA (1974) Real-time scanning-slit confocal microscopy of the in-vivo human cornea. Appl Optics 33:695–701

    Google Scholar 

  35. Masters BR, Thaer AA (1994) In vivo human corneal confocal microscopy of identical fields of subepithelial nerve plexus, basal epithelial, and wing cells at different times. Microsc Res Tech 29:350–356

    Article  CAS  PubMed  Google Scholar 

  36. Masters BR, Böhnke M (2001) Three-dimensional confocal microscopy of the human cornea in vivo. Ophthalmic Res 33:125–135

    Article  CAS  PubMed  Google Scholar 

  37. Mastropasqua L, Nubile M (2002) Confocal microscopy of the cornea. Slack Inc., Thorofare, NJ

    Google Scholar 

  38. Mathers WD, Sutphin JE, Folberg R, Meier PA, Wenzel RP, Elgin RG (1996) Outbreak of keratitis presumed to be caused by Acanthamoeba. Am J Ophthalmol 121:129–142

    CAS  PubMed  Google Scholar 

  39. Mathers WD, Lane JA, Zimmerman MB (1997) Assessment of the tear film with tandem scanning confocal microscopy. Cornea 16:162–168

    CAS  PubMed  Google Scholar 

  40. Mathers WD, Nelson SE, Lane JL, Wilson ME, Allen RC, Folberg R (2000) Confirmation of confocal microscopy diagnosis of Acanthamoeba keratitis using polymerase chain reaction analysis. Arch Ophthalmol 118:178–183

    CAS  PubMed  Google Scholar 

  41. Maurice DM (1968) Cellular membrane activity in the corneal endothelium of the intact eye. Experientia 24:1094–1095

    Article  CAS  PubMed  Google Scholar 

  42. Maurice DM (1967) The use of fluorescein in ophthalmological research. Invest Ophthalmol 6:464–477

    CAS  PubMed  Google Scholar 

  43. Maurice DM (1974) A scanning slit optical microscope. Invest Ophthalmol 13:1033–1037

    CAS  PubMed  Google Scholar 

  44. Moller-Pedersen T, Ehlers N (1995) A three-dimensional study of the human corneal keratocyte density. Curr Eye Res 14:459–464

    CAS  PubMed  Google Scholar 

  45. Moller-Pedersen T, Vogel M, Li HF, Petroll WM, Cavanagh HD, Jester JV (1997) Quantification of stromal thinning, epithelial thickness, and corneal haze after photorefractive keratectomy using in vivo confocal microscopy. Ophthalmology 104:360–368

    CAS  PubMed  Google Scholar 

  46. Moller-Pedersen T, Li HF, Petroll WM, Cavanagh HD, Jester JV (1998) Confocal microscopic characterization of wound repair after photorefractive keratectomy. Invest Ophthalmol Vis Sci 39:487–501

    CAS  PubMed  Google Scholar 

  47. Müller LJ, Marfurt CF, Kruse F, Tervo TM (2003) Corneal nerves: structure, contents and function. Exp Eye Res 76:521–542

    Article  PubMed  Google Scholar 

  48. Müller LJ, Vrensen GF, Pels L, Cardozo BN, Willekens B (1997) Architecture of human corneal nerves. Invest Ophthalmol Vis Sci 38:985–994

    PubMed  Google Scholar 

  49. Naumann OHG (1997) Pathologie des Auges. Springer, Berlin, Heidelberg, New York, pp 22–30

    Google Scholar 

  50. O’Brart DP, Lohmann CP, Fitzke FW, Smith SE, Kerr-Muir MG, Marshall J (1994) Night vision after eximer laser photorefractive keratectomy: haze and halos. Eur J Ophthalmol 4:43–51

    CAS  PubMed  Google Scholar 

  51. Oliveira-Soto L, Efron N (2001) Morphology of corneal nerves using confocal microscopy. Cornea 20:374–384

    Article  CAS  PubMed  Google Scholar 

  52. Parazza F, Humbert C, Usson Y (1993) Method for 3D volumetric analysis of intranuclear fluorescence distribution in confocal microscopy. Comput Med Imaging Graph 17:189–200

    CAS  PubMed  Google Scholar 

  53. Patel SV, McLaren JW, Camp JJ, Nelson LR, Bourne WM (1999) Automated quantification of keratocyte density by using confocal microscopy in vivo. Invest Ophthalmol Vis Sci 40:320–326

    CAS  PubMed  Google Scholar 

  54. Petran M, Hadravsky M, Egger MD, Galambos R (1968) Tandem scanning reflected-light microscope. J Opt Soc Am 58:661–664

    Google Scholar 

  55. Pfister DR, Cameron JD, Krachmer JH, Holland EJ (1996) Confocal microscopy findings of Acanthamoeba keratitis. Am J Ophthalmol 121:119–128

    CAS  PubMed  Google Scholar 

  56. Probst LE, Doane JF (2001) Refractive surgery — a colour synopsis. Thieme, New York

    Google Scholar 

  57. Reinhard T, Godehardt E, Pfahl H-G, Sundmacher R (2000) Lokales Cyclosporin A bei Nummuli nach Keratokonjunktivitis epidemica. Ophthalmologe 97:764–768

    CAS  PubMed  Google Scholar 

  58. Richter A, Slowik C, Somodi S, Vick H-P, Guthoff R (1997) In-vivo-Darstellung der Hornhautinnervation des Menschen mit Hilfe der konfokalen Mikroskopie. Ophthalmologe 94:141–146

    Article  CAS  PubMed  Google Scholar 

  59. Rosenberg ME, Tervo TM, Müller LJ, Moilanen JA, Vesaluoma MH (2002) In vivo confocal microscopy after herpes keratitis. Cornea 21:265–269

    Article  PubMed  Google Scholar 

  60. Ruben M (1978) Kontaktlinsenanpassung. G. Fischer Verlag, Stuttgart, pp 23–45

    Google Scholar 

  61. Sangwan VS (2001) Limbal stem cells in health and disease. Biosci Rep 21:385–405

    Article  CAS  PubMed  Google Scholar 

  62. Scheuerle AF, Schmidt E (2004) Atlas of laser scanning ophthalmoscopy. Springer, Berlin Heidelberg New York

    Google Scholar 

  63. Smithpeter C, Dunn A, Drezek R, Collier T, Richards-Kortum R (1998) Near real time confocal microscopy of cultured amelanotic cells: sources of signal, contrast agents and limits of contrast. J Biomed Opt 3:429–436

    Article  Google Scholar 

  64. Stave J, Slowik C, Somodi S, Hahnel C, Grümmer G, Guthoff R (1998) Keratozytendichte der Invivo-Kornea. Automatische Messung mit einem modifizierten konfokalen Mikroskop Microphthal®. Klin Monatsbl Augenheilkd 213:38–44

    CAS  PubMed  Google Scholar 

  65. Stave J, Guthoff R (1998) Darstellung von Tränenfilm und In-vivo-Kornea. Erste Untersuchungsergebnisse mit einem modifiziertem konfokalen Laser-Scanning-Ophthalmoskop. Ophthalmologe 95:104–109

    Article  CAS  PubMed  Google Scholar 

  66. Stave J, Zinser G, Grümmer G, Guthoff R (2002) Der modifizierte Heidelberg-Retina-Tomograph HRT. Ophthalmologe 99:276–280

    Article  CAS  PubMed  Google Scholar 

  67. Stelzer EH, Wacker I, De Mey JR (1991) Confocal fluorescence microscopy in modern cell biology. Semin Cell Biol 2:145–152

    CAS  PubMed  Google Scholar 

  68. Straub M (1888) Flourescinlösung als diagnostisches Hülfsmittel für Hornhauterkrankungen. Centralbl Augenheilkd 12:75–77

    Google Scholar 

  69. Sundmacher R, Engelskirchen U (1991) Zum Problem der rezidivierenden und persistierenden Nummuli nach Keratokonjunktivitis epidemica. Klin Monatsbl Augenheilkd 198:550–554

    CAS  PubMed  Google Scholar 

  70. Suzuki T, Sano Y, Kinoshita S (2000) Conjunctival inflammation induces Langerhans cell migration into the cornea. Curr Eye Res 21:530–533

    Article  Google Scholar 

  71. Swensson O, Swensson B, Nolle B, Rochels R, Wannke B, Thiel HJ (2000) Keratin-Gen-Mutationen als Ursache der Epitheldystrophie Meesman-Wilke und autosomal dominanter Verhornungsstörungen der Haut. Klin Monatsbl Augenheilkd 217:43–51

    Article  CAS  PubMed  Google Scholar 

  72. Tervo T, Moilanen J (2003) In vivo confocal microscopy for evaluation of wound healing following corneal refractive surgery. Prog Retin Eye Res 22:339–358

    Article  PubMed  Google Scholar 

  73. Thaer A, Geyer OC (1997) Die Anwendung der konfokalen Spaltscanning-Mikroskopie auf die In-vivo-Untersuchunge der Kornea-Mikrostruktur im Zusammenhang mit dem Tragen von Kontaktlinsen. Contactologia 19:157–177

    Google Scholar 

  74. Tomii S, Kinoshita S (1994) Observations of human corneal epithelium by tandem scanning confocal microscope. Scanning 16:305–306

    CAS  PubMed  Google Scholar 

  75. Torens S, Berger E, Stave J, Guthoff R (2000) Darstellung der Mikroarchitektur und Dynamik der Aufreißphänomene des präkornealen Tränenfilms mit Hilfe der Laser-Rastermikroskopie. Ophthalmologe 97:635–639

    Article  CAS  PubMed  Google Scholar 

  76. Torens S, Berger E, Stave J, Guthoff R (2000) Light, reflecting and laser scanning microscopy in the imaging and assessment of the precorneal tear film. New perspectives on the break-up of the tear film. Contactologia 22:49–67

    Google Scholar 

  77. Vesaluoma M, Perez-Santonja J, Petroll WM, Linna T, Alio J, Tervo T (2000) Corneal stromal changes induced by myopic LASIK. Invest Ophthalmol Vis Sci 41:369–376

    CAS  PubMed  Google Scholar 

  78. Vogt A (1921) Lehrbuch und Atlas der Spaltlampenmikroskopie des lebenden Auges. 2nd edn 1930. Springer, Berlin, p 50

    Google Scholar 

  79. Webb RH (1996) Confocal optical microscopy. Rep Prog Phys 59:427–471

    Article  Google Scholar 

  80. Wiegand W, Thaer AA, Kroll P, Geyer OC, Garcia AL (1995) Optical sectioning of the cornea with a new confocal in vivo slit-scanning videomicroscope. Ophthalmology 102:568–575

    CAS  PubMed  Google Scholar 

  81. Wirbelauer C, Scholz C, Engelhardt R, Laqua H, Pham DT (2001) Biomorphometrie des Hornhautepithels mittels spaltlampenadaptierter optischer Kohärenztomographie OCT. Ophthalmologe 98:848–852

    CAS  PubMed  Google Scholar 

  82. Yoon MK, Warren JF, Holsclaw DS, Gritz DC, Margolis TP (2004) A novel arginine substitution mutation in 1A domain and a novel 27 bp insertion mutation in 2B domain of keratin 12 gene associated with Meesmann’s corneal dystrophy. Br J Ophthalmol 88:752–756

    Article  CAS  PubMed  Google Scholar 

  83. Zhivov A, Stave J, Vollmar B, Guthoff RF (2005) In vivo confocal microscopic evaluation of Langerhans cell density and distribution in the normal human corneal epithelium. Graefes Arch Clin Exp Ophthalmol (in press)

    Google Scholar 

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Authors and Affiliations

  1. University Eye Hospital Rostock, Doberaner Strasse 140, 18057, Rostock, Germany

    Rudolf F. Guthoff (Prof. Dr.) & Joachim Stave (Prof. Dr.)

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  1. Rudolf F. Guthoff
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  2. Joachim Stave
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Editors and Affiliations

  1. University Eye Hospital, Killianstrasse 5, 79106, Freiburg, Germany

    Thomas Reinhard MD (Professor of Ophthalmology) (Professor of Ophthalmology)

  2. Cornea & External Diseases Service, Moorfields Eye Hospital, London, EC1V 2PD, UK

    D.F.P. Larkin MD, MRCPF, FRCS

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Guthoff, R.F., Stave, J. (2006). In Vivo Micromorphology of the Cornea: Confocal Microscopy Principles and Clinical Applications. In: Reinhard, T., Larkin, D. (eds) Cornea and External Eye Disease. Essentials in Ophthalmology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-31226-9_13

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