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PC12 Cells as a Model of Neuronal Differentiation

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Cell Culture in the Neurosciences

Part of the book series: Current Topics in Neurobiology ((CTNB))

Abstract

In 1975 Tischler and Greene reported the culture of a norepinephrine producing pheochromocytoma previously observed and carried in New England Deaconess rats by Warren and his co-workers (Warren and Chute, 1972; DeLellis et al., 1973). The tumor cells grew readily under standard culture conditions and exhibited a formaldehyde-induced fluorescence characteristic of the presence of catecholamines. Many of the cells produced short processes which were evident within 24 hr of plating. In the presence of nanogram quantities of nerve growth factor (NGF), more processes were evident and within 1 to 2 weeks of plating there were up to 20 times more processes in the presence of NGF.

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  1. Section on Growth Factors, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, 20205, USA

    Gordon Guroff

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  1. The Marine Biomedical Institute and Department of Human Biological Chemistry and Genetics, The University of Texas Medical Branch at Galveston, Galveston, Texas, USA

    Jane E. Bottenstein

  2. W. Alton Jones Cell Science Center, Inc, Lake Placid, New York, USA

    Gordon Sato

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Guroff, G. (1985). PC12 Cells as a Model of Neuronal Differentiation. In: Bottenstein, J.E., Sato, G. (eds) Cell Culture in the Neurosciences. Current Topics in Neurobiology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2473-7_8

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