Abstract
We present methods, fixation schedules, and stains for preparing whole mounts of murine mammary glands. We have included references to papers published 60 years ago as well as those in current journals. The use of this method has undergone a renaissance with the advent of transgenic and knock-in—knockout genetic mouse models relevant to the study of mammary growth, development, and neoplasia.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
W. U. Gardner and L. C. Strong (1935). The normal development of the mammary glands of virgin female mice of ten strains varying in susceptibility to spontaneous neoplasms. Am. J. Cancer 25: 282–290.
H. C. Taylor and C. A. Waltman (1940). Hyperplasias of the mammary gland in the human being and in the mouse. Morphologic and etiologic contrasts. Arch. Surgery 40: 733–820.
R. A. Huseby and J. J. Bittner (1946). A comparative morphological study of the mammary glands with reference to the known factors influencing the development of mammary carcinoma in mice. Cancer Res. 6: 240–255.
A. J. Dalton (1945). Histogenesis of the Mammary Gland of the Mouse, The Science Press Printing Company, Lancaster, PA.
K. B. DeOme, L. J. Faulkin, H. A. Bern, and P. B. Blair (1959). Development of mammary tumors from hyperplastic alveolar nodules transplanted into gland-free mammary fat pads of female C3H mice. Cancer Res. 19: 515–520.
R. V. Nie and A. Dux (1971). Biological and morphological characteristics of mammary tumors in GR mice. J. Natl. Cancer Inst. 46 (4): 885–897.
C. W Daniel, K. B. DeOme, J. T. Young, P. B. Blair, and L. J. Faulkin, Jr. (1968). The in vivo life span of normal and preneoplastic mouse mammary glands: a serial transplantation study. Proc. Natl. Acad. Sci. USA 61 (1): 53–60.
E. M. Rivera (1971). Mammary Gland Culture, Freeman, San Francisco.
M. R. Banerjee, B. G. Wood, E K. Lin, and L. R. Crump (1976). Organ culture of the whole mammary glands of the mouse. Tissue Culture Associ. Manual 2 (4): 457–462.
D. Medina (1973). Preneoplastic lesions in mouse mammary tumorigenesis. Metho. Cancer Res. 7: 3–53.
H. Nagasawa and R. Yanai (1978). Normal and Abnormal Growth of the Mammary Gland, Japan Scientific Societies Press, Tokyo.
G. H. Smith, D. Gallahan, E Diella, C. Jhappan, G. Merlino, and R. Callahan (1995). Constitutive expression of a truncated INT3 gene in mouse mammary epithelium impairs differentiation and functional development. Cell Growth Differentiation 6: 563–577.
D. Gallahan, C. Jhappan, G. Robinson, L. Hennighausen, R. Sharp, E. Kordon, R. Callahan, G. Merlin, and G. H. Smith (1996). Expression of a truncated Int3 gene in developing secretory mammary epithelium specifically retards lobular differentiation resulting in tumorigenesis. Cancer Res. 56: 1775–1785.
J. K. Presnell and M. P. Schreibman (1997). Humason’s Animal Tissue Techniques, The John Hopkins University Press, Baltimore, MD.
E. C. Clayden (1971). Practical Section Cutting and Staining, Churchill and Livingstone, London.
H. A. Davenport (1960). Histological and Histochemical Technics, W.B. Saunders Company, Philadelphia, PA.
L. G. Lima (ed.) (1968). Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology, McGraw-Hill, New York.
J. B. Carnoy (1887). Cellule 3: 247–324.
J. M. Strum (1979). A mammary gland whole mount technique that preserves cell fine structure for electron microscopy. J. Histochem. Cytochem. 27 (9): 1271–1274.
M. Heidenhain (1892). Ueber Kern and Protoplasma, Wilhelm Englemann, Leipsig.
E Mayer (1899). Üeber haematoxylin, carmin, and verwandte materien. Z. WIss. Mikrosk. 16: 196–220.
M. A. Hayat (1993). Stains and Cytochemical Methods, Plenum Press, New York.
K. P. Hummel, E L. Richardson, and E. Fekete (1966). Anatomy. In E. L. Green (ed.), Biology of the Laboratory Mouse, McGraw Hill, New York, p. 267.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2000 Springer Science+Business Media New York
About this chapter
Cite this chapter
Rasmussen, S.B., Young, L.J.T., Smith, G.H. (2000). Preparing Mammary Gland Whole Mounts from Mice. In: Ip, M.M., Asch, B.B. (eds) Methods in Mammary Gland Biology and Breast Cancer Research. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4295-7_7
Download citation
DOI: https://doi.org/10.1007/978-1-4615-4295-7_7
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-6927-1
Online ISBN: 978-1-4615-4295-7
eBook Packages: Springer Book Archive