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Advances in Neurochemistry pp 1–82Cite as

The Radioactive Deoxyglucose Method

Theory, Procedure, and Applications for the Measurement of Local Glucose Utilization in the Central Nervous System

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Part of the book series: Advances in Neurochemistry ((ANCH,volume 4))

Abstract

The brain is a complex, heterogeneous organ composed of many anatomical and functional components with markedly different levels of functional activity that vary independently with time and function. Other tissues are generally far more homogeneous with most of their cells functioning similarly and synchronously in response to a common stimulus or regulatory influence. The central nervous system, however, consists of innumerable subunits each integrated into its own set of functional pathways and networks and subserving only one or a few of the many activities in which the nervous system participates. Understanding how the nervous system functions requires knowledge not only of the mechanisms of excitation and inhibition but even more so of their precise localization in the nervous system and the relationships of neural subunits to specific functions.

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Abbreviations

DG:

2-deoxyglucose

DG-6-P:

2-deoxyglucose-6-phosphate

G-6-P:

glucose-6-phosphate

References

  • Albers, R. W., 1967, Biochemical aspects of active transport, Annu. Rev. Biochem. 36: 727–756.

    Article  PubMed  CAS  Google Scholar 

  • Altenau, L. L., and Agranoff, B. W., 1978, A sequential double-label 2-deoxyglucose method for measuring regional cerebral metabolism, Brain Res. 153: 375–381.

    Article  PubMed  CAS  Google Scholar 

  • Bachelard, H. S., 1971, Specificity and kinetic properties of monosaccharide uptake into guinea pig cerebral cortex in vitro, J. Neurochem. 18: 213–222.

    Article  PubMed  CAS  Google Scholar 

  • Bachelard, H. S., Clark, A. G., and Thompson, M. F., 1971, Cerebral-cortex hexokinases. Elucidation of reaction mechanisms by substrate and dead-end inhibitor kinetic analysis, Biochem. J. 123: 707–715.

    PubMed  CAS  Google Scholar 

  • Ballas, L. M., and Arion, W. J., 1977, Measurement of glucose 6-phosphate penetration into liver microsomes. Confirmation of substrate transport in the glucose-6-phosphatase system, J. Biol. Chem. 252: 8512–8518.

    PubMed  CAS  Google Scholar 

  • Batipps, M., Miyaoka, M., Shinohara, M., Sokoloff, L., and Kennedy, C., 1981, Comparative rates of local cerebral glucose utilization in the visual system of conscious albino and pigmented rats, Neurology 31: 58–62.

    Article  PubMed  CAS  Google Scholar 

  • Bidder, T. G., 1968, Hexose translocation across the blood—brain interface: Configurational aspects, J. Neurochem. 15: 867–874.

    Article  PubMed  CAS  Google Scholar 

  • Birren, J. E., Butler, R. N., Greenhouse, S. W., Sokoloff, L., and Yarrow, M. R. (eds.), 1963, Human Aging: A Biological and Behavioral Study, Public Health Service Publication No. 986, US Government Printing Office, Washington DC.

    Google Scholar 

  • Brown, L., and Wolfson, L., 1978, Apomorphine increases glucose utilization in the substantia nigra, subthalamic nucleus, and corpus striatum of the rat, Brain Res. 148: 188–193.

    Article  Google Scholar 

  • Caldwell, P. C., 1968, Factors governing movement and distribution of inorganic ions in nerve and muscle, Physiol. Rev. 48: 1–64.

    PubMed  CAS  Google Scholar 

  • Caveness, W. F., 1969, Ontogeny of focal seizures, in: Basic Mechanisms of the Epilepsies ( H. H. Jasper, A. A. Ward, and A. Pope, eds.), pp. 517–534, Little, Brown and Co., Boston.

    Google Scholar 

  • Caveness, W. F., 1980, Appendix: Tables of local cerebral glucose utilization in various experimental preparations, Ann. Neurol. 7: 230–237.

    Article  Google Scholar 

  • Caveness, W. F., Kato, M., Malamut, B. L., Hosokawa, S., Wakisaka, S., and O’Neill, R. R., 1980, Propagation of focal motor seizures in the pubescent moneky, Ann. Neurol. 7: 213–221

    Article  PubMed  CAS  Google Scholar 

  • Collins, R. C., Kennedy, C., Sokoloff, L., and Plum, F., 1976, Metabolic anatomy of focal motor seizures, Arch. Neurol. (Chicago) 33: 536–542.

    Article  CAS  Google Scholar 

  • Des Rosiers, M. H., Kennedy, C., Shinohara, M., and Sokoloff, L., 1976, Effects of CO, on local cerebral glucose utilization in the conscious rat, Neurology 26: 346.

    Google Scholar 

  • Des Rosiers, M. H., Sakurada, O., Jehle, J., Shinohara, M., Kennedy, C., and Sokoloff, L., 1978, Functional plasticity in the immature striate cortex of the monkey shown by the [14C]deoxyglucose method, Science 200: 447–449.

    Article  PubMed  Google Scholar 

  • Des Rosiers, M. H., and Descarries, L., 1978, Adaptation de la méthode au désoxyglucose à l’échelle cellulaire: préparation histologique du système nerveux central en vue de la radio-autographie à haute résolution, C. R. Acad. Sci. Ser. D 287: 153–156.

    Google Scholar 

  • Dixon, M., and Webb, E. C., 1964, Enzymes, 2nd ed., pp. 84–87, Academic Press, New York.

    Google Scholar 

  • Duffy, T. E., Cavazzuti, M., Gregoire, N. M., Cruz, N. F., Kennedy, C., and Sokoloff, L., 1979, Regional cerebral glucose metabolism in newborn beagle dogs, Trans. Am. Soc. Neurochem. 10: 171.

    Google Scholar 

  • Durham, D., and Woolsey, T. A., 1977, Barrels and columnar cortical organization: Evidence from 2-deoxyglucose (2-DG) experiments, Brain Res. 137: 169–174.

    Article  PubMed  CAS  Google Scholar 

  • Eklöf, B., Lassen, N. A., Nilsson, L., Norberg, K., and Siesjö, B. K., 1973, Blood flow and metabolic rate for oxygen in the cerebral cortex of the rat, Acta Physiol. Scand. 88: 587–589.

    Article  PubMed  Google Scholar 

  • Engel, J., Jr., Wolfson, L., and Brown, L., 1978, Anatomical correlates of electrical and behavioral events related to amygdaloid kindling, Ann. Neurol. 3: 538–544.

    Article  PubMed  Google Scholar 

  • Freygang, W. H., Jr., and Sokoloff, L., 1958, Quantitative measurement of regional circulation in the central nervous system by the use of radioactive inert gas, Adv. Biol. Med. Phys. 6: 263–279.

    PubMed  CAS  Google Scholar 

  • Friedli, C., 1977, Kinetics of changes in p02 and extracellular potassium activity in stimulated rat sympathetic ganglia, in: Advances in Experimental Medicine and Biology, Oxygen Transport to Tissue Ill ( I. A. Silver, M. Erecinska and H. I. Bicher, eds.), pp. 747–754, Plenum Press, New York.

    Google Scholar 

  • Galvan, M., Ten Bruggencate, G., and Senekowitsch, R., 1979, The effects of neuronal stimulation and oubain upon extracellular K+ and Ca2+ levels in rat isolated sympathetic ganglia, Brain Res. 160: 544–548.

    Article  PubMed  CAS  Google Scholar 

  • Gjedde, A., Caronna, J. J., Hindfelt, B., and Plum, F., 1975, Whole-brain blood flow and oxygen metabolism in the rat during nitrous oxide anesthesia, Am. J. Physiol. 229: 113–118.

    PubMed  CAS  Google Scholar 

  • Goochee, C., Rasband, W., and Sokoloff, L., 1980, Computerized densitometry and color coding of [14C]deoxyglucose autoradiographs, Ann. Neurol. 7: 359–370.

    Article  PubMed  CAS  Google Scholar 

  • Hand, P. J., Greenberg, J. H., Miselis, R. R., Weller, W. L., and Reivich, M., 1978, A normal and altered cortical column: a quantitative and qualitative (14C)-2 deoxyglucose (2DG) mapping study, Neurosci. Abstr. 4: 553.

    Google Scholar 

  • Hawkins, R. A., and Miller, A. L., 1978, Loss of radioactive 2-deoxy-D-glucose-6-phosphate from brains of conscious rats: Implications for quantitative autoradiographic determination of regional glucose utilization, Neuroscience 3: 251–258.

    Article  PubMed  CAS  Google Scholar 

  • Hawkins, R. A., Miller, A. L., Cremer, J. E., and Veech, R. L., 1974, Measurement of the rate of glucose utilization by rat brain in vivo, J. Neurochem. 23: 917–923.

    Article  PubMed  CAS  Google Scholar 

  • Hers, H. G., 1957, Le Métabolisme du Fructose, p. 102, Arscia, Brussels.

    Google Scholar 

  • Hers, H. G., and deDuve, C., 1950, Le système hexose-phosphatasique. II. Repartition de l’activite glucose-6-phosphatasique dans les tissus, Bull. Soc. Chim. Biol. 32: 20–29.

    PubMed  CAS  Google Scholar 

  • Horowicz, P., and Larrabee, M. G., 1958, Glucose consumption and lactate production in a mammalian sympathetic ganglion at rest and in activity, J. Neurochem. 2: 102–118.

    Article  PubMed  CAS  Google Scholar 

  • Horton, R. W., Meldrum, B. S., and Bachelard, H. S., 1973, Enzymic and cerebral metabolic effects of 2-deoxy-D-glucose, J. Neurochem. 21: 507–520.

    Article  PubMed  CAS  Google Scholar 

  • Hosokawa, S., Iguchi, T., Caveness, W. F., Kato, M., O’Neill, R. R., Wakisaka, S., and Mala-mut, B. L., 1980, Effects of manipulation of the sensorimotor system on focal motor seizures in the monkey, Ann. Neurol. 7: 222–229.

    Article  PubMed  CAS  Google Scholar 

  • Hubel, D. H., and Wiesel, T. N., 1968, Receptive fields and functional architecture of monkey striate cortex, J. Physiol. 195: 215–243.

    PubMed  CAS  Google Scholar 

  • Hubel, D. H., and Wiesel, T. N., 1972, Laminar and columnar distribution of geniculo-cortical fibers in the Macaque monkey, J. Comp. Neurol. 146: 421–450.

    Article  PubMed  CAS  Google Scholar 

  • Hubel, D. H., Wiesel, T. N., and Stryker, M. P., 1978, Anatomical demonstration of orientation columns in macaque monkey, J. Comp. Neurol. 177: 361–380.

    Article  PubMed  CAS  Google Scholar 

  • Jarvis, C. D., Mishkin, M., Shinohara, M., Sakurada, O., Miyaoka, M., and Kennedy, C., 1978, Mapping the primate visual system with the [1°C] 2-deoxyglucose technique, Neurosci. Abstr. 4: 632.

    Google Scholar 

  • Kato, M., Malamut, B. L., Caveness, W. F., Hosokawa, S., Wakisaka, S., and O’Neill, R. R., 1980, Local cerebral glucose utilization in newborn and pubescent monkeys during focal motor seizures, Ann. Neurol. 7: 204–212.

    Article  PubMed  CAS  Google Scholar 

  • Kennedy, C., Des Rosiers, M., Jehle, J. W., Reivich, M., Sharp, F., and Sokoloff, L., 1975, Mapping of functional neural pathways by autoradiographic survey of local metabolic rate with [14C]deoxyglucose, Science 187: 850–853.

    Google Scholar 

  • Kennedy, C., Des Rosiers, M. H., Sakurada, O., Shinohara, M., Reivich, M., Jehle, J. W., and Sokoloff, L., 1976, Metabolic mapping of the primary visual system of the monkey by means of the autoradiographic [14C]deoxyglucose technique, Proc. Natl. Acad. Sci. USA 73: 4230–4234.

    Article  PubMed  CAS  Google Scholar 

  • Kennedy, C., Sakurada, O., Shinohara, M., Jehle, J., and Sokoloff, L., 1978, Local cerebral glucose utilization in the normal conscious Macaque monkey, Ann. Neurol. 4: 293–301.

    Article  PubMed  CAS  Google Scholar 

  • Kety, S. S., 1950, Circulation and metabolism of the human brain in health and disease, Am. J. Med. 8: 205–217.

    Article  PubMed  CAS  Google Scholar 

  • Kety, S. S., 1957, The general metabolism of the brain in vivo, in: Metabolism of the Nervous System ( D. Richter, ed.), pp. 221–237, Pergamon Press, London.

    Google Scholar 

  • Kety, S. S., 1960, Measurement of local blood flow by the exchange of an inert, diffusible substance, Methods Med. Res. 8: 228–236.

    Google Scholar 

  • Kety, S. S., and Schmidt, C. F., 1948a, The nitrous oxide method for the quantitative determination of cerebral blood flow in man: theory, procedure, and normal values, J. Clin. Invest. 27: 476–483.

    Article  PubMed  CAS  Google Scholar 

  • Kety, S. S., and Schmidt, C. F., 1948b, Effects of altered arterial tensions of carbon dioxide and oxygen on cerebral blood flow and cerebral oxygen consumption of normal young men, J. Clin. Invest. 27: 484–492.

    Article  PubMed  CAS  Google Scholar 

  • Knott, G. D., and Reece, D. K., 1972, mlab: A civilized curve-fitting system, in: Proceedings of the online ’72 International Conference, Vol. 1, pp. 497–526, Brunel University, England.

    Google Scholar 

  • Knott, G. D., and Shrager, R. I., 1972, On-line modeling by curve-fitting, in: Computer Graphics: Proceedings of the siggraph Computers in Medicine Symposium, Vol. 6, No. 4, pp. 138–151, Association for Computing Machinery, siggraph Notices.

    Google Scholar 

  • Kuhl, D., Engel, J., Phelps, M., and Selin, C., 1979, Patterns of local cerebral metabolism and perfusion in partial epilepsy by emission computed tomography of’ $F-flurodeoxyglucose and “N-ammonia, Acta Neurol. Scand. Suppl. 60: 538–539.

    Google Scholar 

  • Landau, B. R., and Lubs, H. A., 1958, Animal response to 2-deoxy-D-glucose administration, Proc. Soc. Exp. Biol. 99: 124–127.

    PubMed  CAS  Google Scholar 

  • Landau, W. M., Freygang, W. H., Jr., Rowland, L. P., Sokoloff, L., and Kety, S. S., 1955, The local circulation of the living brain; Values in the unanesthetized and anesthetized cat, Trans. Am. Neurol. Assoc. 80: 125–129.

    Google Scholar 

  • Larrabee, M. G., 1958, Oxygen consumption of excised sympathetic ganglia at rest and in activity, J. Neurochem. 2: 81–101.

    Article  PubMed  CAS  Google Scholar 

  • Lashley, K. S., 1934, The mechanism of vision. VII. The projection of the retina upon the primary optic centers of the rat, J. Comp. Neurol. 59: 341–373.

    Article  Google Scholar 

  • Lassen, N. A., 1959, Cerebral blood flow and oxygen consumption in man, Physiol. Rev. 39: 183–238.

    PubMed  CAS  Google Scholar 

  • Lassen, N. A., and Munck, O., 1955, The cerebral blood flow in man determined by the use of radioactive krypton, Acta Physiol. Scand. 33: 30–49.

    Article  PubMed  CAS  Google Scholar 

  • Mata, M., Fink, D. J., Gainer, H., Smith, C. B., Davidsen, L., Savaki, H., Schwartz, W. J., and Sokoloff, L., 1980, Activity-dependent energy metabolism in rat posterior pituitary reflects sodium pump activity, J. Neurochem. 34: 213–215.

    Article  PubMed  CAS  Google Scholar 

  • Meldrum, B. S., and Horton, R. W., 1973, Cerebral functional effects of 2-deoxy-o-glucose and 3-O-methylglucose in Rhesus monkeys, Electroencephalogr. Clin. Neurophysiol. 35: 59–66.

    Article  PubMed  CAS  Google Scholar 

  • Miyaoka, M., Shinohara, M., Batipps, M., Pettigrew, K. D., Kennedy, C., and Sokoloff, L., 1979a, The relationship between the intensity of the stimulus and the metabolic response in the visual system of the rat, Acta Neurol. Scand. Suppl. 60: 16–17.

    Google Scholar 

  • Miyaoka, M., Shinohara, M., Kennedy, C., and Sokoloff, L., 19796, Alterations in local cerebral glucose utilization (LCGU) in rat brain during hypoxemia, Trans. Am. Neurol. Assoc. 104: 151–154.

    Google Scholar 

  • Montero, V. M., and Guillery, R. W., 1968, Degeneration in the dorsal lateral geniculate nucleus of the rat following interruption of the retinal or cortical connections, J. Comp. Neurol. 134: 211–242.

    Article  PubMed  CAS  Google Scholar 

  • Nordlie, R. C., 1971, Glucose-6-phosphatase, hydrolytic and synthetic activities, in: The Enzymes 3rd ed., Vol. IV ( P. D. Boyer, ed.), pp. 543–610, Academic Press, New York.

    Google Scholar 

  • Nordlie, R. C., 1974, Metabolic regulation by multifunctional glucose-6-phosphatase, Curr. Top. Cell Regul. 8: 33–117.

    PubMed  CAS  Google Scholar 

  • Nordmann, J. J., 1977, Ultrastructural morphometry of the rat neurohypophysis, J. Anat. 123: 213–218.

    PubMed  CAS  Google Scholar 

  • Oldendorf, W. H., 1971, Brain uptake of radiolabeled amino acids, amines, and hexoses after arterial injection, Am. J. Physiol. 221: 1629–1638.

    PubMed  CAS  Google Scholar 

  • Pappius, H. M., Savaki, H. E., Fieschi, C., Rapoport, S. I., and Sokoloff, L., 1979, Osmotic opening of the blood-brain barrier and local cerebral glucose utilization, Ann. Neurol. 5: 211–219.

    Article  PubMed  CAS  Google Scholar 

  • Patlak, C. S., and Pettigrew, K. D., 1976, A method to obtain infusion schedules for prescribed blood concentration time courses, J. Appl. Physiol. 40: 458–463.

    PubMed  CAS  Google Scholar 

  • Phelps, M. E., Huang, S. C., Hoffman, E. J., Selin, C., Sokoloff, L., and Kuhl, D. E., 1979, Tomographic measurement of local cerebral glucose metabolic rate in humans with (F-18)2fluoro-2-deoxy-d-glucose: Validation of method, Ann. Neurol. 6: 371–388.

    Article  PubMed  CAS  Google Scholar 

  • Plum, F., Gjedde, A., and Samson, F. E., 1976, Neuroanatomical mapping by the radioactive 2deoxy-D-glucose method, Neurosci. Res. Program Bull. 14: 457–518.

    Google Scholar 

  • Post, R. M., Kennedy, C., Shinohara, M., Squillace, K., Miyaoka, M., Suda, S., Ingvar, D. H., and Sokoloff, L., 1979, Local cerebral glucose utilization in lidocaine-kindled seizures, Neurosci. Abstr. 5: 196.

    Google Scholar 

  • Prasannan, K. G., and Subrahmanyam, K., 1968, Effect of insulin on the synthesis of glucogen in cerebral cortical slices of alloxan diabetic rats, Endocrinology 82: 1–6.

    Article  PubMed  CAS  Google Scholar 

  • Pulsinelli, W. A., and Duffy, T. E., 1979, Local cerebral glucose metabolism during controlled hypoxemia in rats, Science 204: 626–629.

    Article  PubMed  CAS  Google Scholar 

  • Raggi, F., Kronfeld, D. S., and Kleiber, M., 1960, Glucose-6-phosphatase activity in various sheep tissues, Proc. Soc. Exp. Biol. Med. 105: 485–486.

    PubMed  CAS  Google Scholar 

  • Rakic, P., 1976, Prenatal genesis of connections subserving ocular dominance in the rhesus monkey, Nature 261: 467–471.

    Article  PubMed  CAS  Google Scholar 

  • Reivich, M., Jehle, J., Sokoloff, L., and Kety, S. S., 1969, Measurement of regional cerebral blood flow with antipyrine-[14C] in awake cats, J. Appl. Physiol. 27: 296–300.

    PubMed  CAS  Google Scholar 

  • Reivich, M., Kuhl, D., Wolf, A., Greenberg, J., Phelps, M., Ido, T., Cassella, V., Fowler, J., Hoffman, E., Alavi, A., Som, P., and Sokoloff, L., 1979, The [18F]fluoro-deoxyglucose method for the measurement of local cerebral glucose utilization in man, Circ. Res. 44:127-137.

    Google Scholar 

  • Roth, R. H., 1976, Striatal dopamine and gamma-hydroxybutyrate, Pharmacol. Ther. 2: 71–88.

    CAS  Google Scholar 

  • Roth, R. H., and Giarman, N. J., 1966, y-Butyrolactone and -y-hydroxybutyric acid—I. Distribution and metabolism, Biochem. Pharmacol. 15: 1333–1348.

    Google Scholar 

  • Sacks, W., 1957, Cerebral metabolism of isotopic glucose in normal human subjects, J. Appl. Physiol. 10: 37–44.

    PubMed  CAS  Google Scholar 

  • Sakurada, O., Shinohara, M., Klee, W. A., Kennedy, C., and Sokoloff, L., 1976, Local cerebral glucose utilization following acute chronic morphine administration and withdrawal, Neurosci. Abstr. 2: 613.

    Google Scholar 

  • Savaki, H. E., Kadekaro, M., Jehle, J., and Sokoloff, L., 1978, a-and 3-adrenoreceptor blockers have opposite effects on energy metabolism of the central auditory system, Nature 276: 521–523.

    Google Scholar 

  • Savaki, H. E., Davidsen, L., Smith, C., and Sokoloff, L., 1980, Measurement of free glucose turnover in brain, J. Neurochem. 35: 495–502.

    Article  PubMed  CAS  Google Scholar 

  • Scheinberg, P., and Stead, E. A., Jr., 1949, The cerebral blood flow in male subjects as measured by the nitrous oxide technique. Normal values for blood flow, oxygen utilization, and peripheral resistance, with observations on the effect of tilting and anxiety, J. Clin. Invest. 28: 1163–1171.

    Article  PubMed  CAS  Google Scholar 

  • Schwartz, W. J., 1978, A role for the dopaminergic nigrostriatal bundle in the pathogenesis of altered brain glucose consumption after lateral hypothalamic lesions. Evidence using the “C-labeled deoxyglucose technique, Brain Res. 158: 129–147.

    Article  PubMed  CAS  Google Scholar 

  • Schwartz, W. J., and Gainer, H., 1977, Suprachiasmatic nucleus: use of “C-labeled deoxyglucose uptake as a functional marker, Science 197: 1089–1091.

    Article  PubMed  CAS  Google Scholar 

  • Schwartz, W. J., Sharp, F. R., Gunn, R. H., and Evarts, E. V., 1976, Lesions of ascending dopaminergic pathways decrease forebrain glucose uptake, Nature (London) 261: 155–157.

    Article  CAS  Google Scholar 

  • Schwartz, W. J., Smith, C. B., Davidsen, L., Savaki, H., Sokoloff, L., Mata, M., Fink, D. J., and Gainer, H., 1979, Metabolic mapping of functional activity in the hypothalamoneurohypophysial system of the rat, Science 205: 723–725.

    Article  PubMed  CAS  Google Scholar 

  • Schwartz, W. J., Davidsen, L. C., and Smith, C. B., 1980, In vivo metabolic activity of a putative circadian oscillator, the rat suprachiasmatic nucleus, J. Comp. Neurol. 189: 157–167.

    CAS  Google Scholar 

  • Shapiro, H. M., Greenberg, J. H., Reivich, M., Shipko, E., Van Horn, K., and Sokoloff, L., 1975, Local cerebral glucose utilization during anesthesia, in: Blood Flow and Metabolism in the Brain (A. M. Harper, W. B. Jennett, J. D. Miller and J. O. Rowan, eds.), pp. 9.42–9. 43, Churchill Livingstone, Edinburgh.

    Google Scholar 

  • Sharp, F. R., Kauer, J. S., and Shepherd, G. M., 1975, Local sites of activity-related glucose metabolism in rat olfactory bulb during olfactory stimulation, Brain Res. 98: 596–600.

    Article  PubMed  CAS  Google Scholar 

  • Shinohara, M., Sakurada, O., Jehle, J., and Sokoloff, L., 1976, Effects of D-lysergic acid diethylamide on local cerebral glucose utilization in the rat, Neurosci. Abstr. 2: 615.

    Google Scholar 

  • Shinohara, M., Dollinger, B., Brown, G., Rapoport, S., and Sokoloff, L., 1979, Cerebral glucose utlization: Local changes during and after recovery from spreading cortical depression, Science 203: 188–190.

    Article  PubMed  CAS  Google Scholar 

  • Silverman, M. S., Hendrickson, A. E., and Clopton, B. M., 1977, Mapping of the tonotopic organization of the auditory system by uptake of radioactive metabolities, Neurosci. Abstr. 3: 11.

    Google Scholar 

  • Smith, C. B., Goochee, C., Rapoport, S. I., and Sokoloff, L., 1980, Effects of ageing on local rates of cerebral glucose utilization in the rat, Brain 103: 351–365.

    Article  PubMed  CAS  Google Scholar 

  • Sokoloff, L., 1960, Metabolism of the central nervous system in vivo, in: Handbook of Physiology-Neurophysiology, Vol. III (J. Field, H. W. Magoun and V. E. Hall, eds.) pp. 18431864, American Physiological Society, Washington, D.C.

    Google Scholar 

  • Sokoloff, L., 1966, Cerebral circulatory and metabolic changes associated with aging, Res. Publ. Res. Assoc. Nerv. Ment. Dis. 41: 237–254.

    CAS  Google Scholar 

  • Sokoloff, L., 1969, Cerebral circulation and behavior in man: strategy and findings, in: Psycho-chemical Research in Man ( A. J. Mandell and M. P. Mandell, eds.), pp. 237–252, Academic Press, New York.

    Google Scholar 

  • Sokoloff, L., 1976, Circulation and energy metabolism of the brain, in: Basic Neurochemistry 2nd ed. ( G. J. Siegel, R. W. Albers, R. Katzman, and B. W. Agranoff, eds.), pp. 388–413, Little, Brown and Company, Boston.

    Google Scholar 

  • Sokoloff, L., 1977, Relation between physiological function and energy metabolism in the central nervous system, J. Neurochem. 29: 13–26.

    Article  PubMed  CAS  Google Scholar 

  • Sokoloff, L., 1978, Mapping cerebral functional activity with radioactive deoxyglucose, Trends Neurosci. 1 (3): 75–79.

    Article  CAS  Google Scholar 

  • Sokoloff, L., 1979, The [14C] deoxyglucose method: four years later, Acta Neurol. Scand. Suppl. 60: 640–649.

    Article  Google Scholar 

  • Sokoloff, L., Reivich, M., Kennedy, C., Des Rosiers, M. H., Patlak, C. S., Pettigrew, K. D., Sakurada, O., and Shinohara, M., 1977, The [14C]deoxyglucose method for the measurement of local cerebral glucose utilization: theory, procedure, and normal values in the conscious and anesthetized albino rat, J. Neurochem. 28: 897–916.

    Article  PubMed  CAS  Google Scholar 

  • Sols, A., and Crane, R. K., 1954, Substrate specificity of brain hexokinase, J. Biol. Chem. 210: 581–595.

    PubMed  CAS  Google Scholar 

  • Tower, D. B., 1958, The effects of 2-deoxy-o-glucose on metabolism of slices of cerebral cortex incubated in vitro, J. Neurochem. 3: 185–205.

    Article  CAS  Google Scholar 

  • Webster, W. R., Serviere, J., Batini, C., and LaPlante, S., 1978, Autoradiographic demonstration with 2-[1°C] deoxyglucose of frequency selectivity in the auditory system of cats under conditions of functional activity, Neurosci. Lett. 10: 43–48.

    Article  PubMed  CAS  Google Scholar 

  • Wechsler, L. R., Savaki, H. E., and Sokoloff, L., 1979, Effects of d-and /-amphetamine on local cerebral glucose utilization in the conscious rat, J. Neurochem. 32: 15–22.

    Article  PubMed  CAS  Google Scholar 

  • Whittam, R., 1962, The dependence of the respiration of brain cortex on active cation transport, Biochem. J. 82: 205–212.

    PubMed  CAS  Google Scholar 

  • Wick, A. N., Drury, D. R., Nakada, H. I., and Wolfe, J. B., 1957, Localization of the primary metabolic block produced by 2-deoxyglucose, J. Biol. Chem. 224: 963–969.

    PubMed  CAS  Google Scholar 

  • Wiesel, T. N., Hubel, D. H., and Lam, D. M. K., 1974, Autoradiographic demonstration of ocular dominance columns in the monkey striate cortex by means of transneuronal transport, Brain Res. 79: 273–279.

    Article  PubMed  CAS  Google Scholar 

  • Wolfson, L. I., Sakurada, O., and Sokoloff, L., 1977, Effects of y-butyrolactone on local cerebral glucose utilization in the rat, J. Neurochem. 29: 777–783.

    Article  PubMed  CAS  Google Scholar 

  • Yarowsky, P. J., Jehle, J., Ingvar, D. H., and Sokoloff, L., 1979, Relationship between functional activity and glucose utilization in the rat superior cervical ganglion in vivo, Neurosci. Abstr. 5: 421.

    Google Scholar 

  • Yarowsky, P. J., Crane, A. M., and Sokoloff, L., 1980, Stimulation of neuronal glucose utilization by antidromic electrical stimulation in the superior cervical ganglion of the rat, Neurosci. Abstr. 6: 340.

    Google Scholar 

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Authors and Affiliations

  1. Laboratory of Cerebral Metabolism, National Institute of Mental Health, Bethesda, Maryland, USA

    Louis Sokoloff

  2. Department of Health and Human Services, U.S. Public Health Service, Bethesda, Maryland, USA

    Louis Sokoloff

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  1. Louis Sokoloff
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Editors and Affiliations

  1. Mental Health Research Institute and Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan, USA

    B. W. Agranoff

  2. Institute of Psychiatric Research and Department of Psychiatry and Biochemistry, Indiana University School of Medicine, Indianapolis, Indiana, USA

    M. H. Aprison

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© 1982 Plenum Press, New York

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Sokoloff, L. (1982). The Radioactive Deoxyglucose Method. In: Agranoff, B.W., Aprison, M.H. (eds) Advances in Neurochemistry. Advances in Neurochemistry, vol 4. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-7541-8_1

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  • DOI: https://doi.org/10.1007/978-1-4684-7541-8_1

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-7543-2

  • Online ISBN: 978-1-4684-7541-8

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