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Ultrasound-Targeted Microbubble Destruction for Cardiac Gene Delivery

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Cardiac Gene Therapy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1521))

Abstract

Ultrasound targeted microbubble destruction (UTMD) is a novel technique that is used to deliver a gene or other bioactive substance to organs of living animals in a noninvasive manner. Plasmid DNA binding with cationic liposome into nanoparticles are assembled into the shell of microbubbles, which are circulated by intravenous injection. Intermittent bursts of ultrasound with low frequency and high mechanical index destroys the microbubbles and releases the nanoparticles into targeted organ to transfect local organ cells. Cell-specific promoters can be used to further enhance cell specificity. Here we describe UTMD applied to cardiac gene delivery.

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Acknowledgment

This work was supported by the Mark Shepherd Endowment of The Baylor Foundation

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Authors and Affiliations

  1. Division of Cardiology, Department of Internal Medicine, Baylor Heart and Vascular Institute, Baylor University Medical Center, 621 N. Hall St, Suite H030, Dallas, TX, 75226, USA

    Shuyuan Chen & Paul A. Grayburn M.D.

Authors
  1. Shuyuan Chen
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  2. Paul A. Grayburn M.D.
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Corresponding author

Correspondence to Paul A. Grayburn M.D. .

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Editors and Affiliations

  1. Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, New York, New York, USA

    Kiyotake Ishikawa

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Chen, S., Grayburn, P.A. (2017). Ultrasound-Targeted Microbubble Destruction for Cardiac Gene Delivery. In: Ishikawa, K. (eds) Cardiac Gene Therapy. Methods in Molecular Biology, vol 1521. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6588-5_14

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  • DOI: https://doi.org/10.1007/978-1-4939-6588-5_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6586-1

  • Online ISBN: 978-1-4939-6588-5

  • eBook Packages: Springer Protocols

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