Abstract
Colony forming or clonogenic assay is an in vitro quantitative technique to examine the capability of a single cell to grow into a large colony through clonal expansion. Clonogenic activity is a sensitive indicator of undifferentiated cancer stem cells. Here, we described the colony forming ability of the isolated breast cancer stem cells from the total population of cancer cells using double-layered, soft agarose-based assay. This method demonstrates that cancer stem cells can survive and generate colony growth in an anchorage-independent culture model. The 0.005% crystal violet solution is used in this assay to visualize the generated colonies.
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Acknowledgment
This work was supported by the startup support from Linkoping University and Cancerfonden.
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Rajendran, V., Jain, M.V. (2018). In Vitro Tumorigenic Assay: Colony Forming Assay for Cancer Stem Cells. In: Papaccio, G., Desiderio, V. (eds) Cancer Stem Cells. Methods in Molecular Biology, vol 1692. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7401-6_8
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DOI: https://doi.org/10.1007/978-1-4939-7401-6_8
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Online ISBN: 978-1-4939-7401-6
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