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Mouse Cell Culture pp 185–196Cite as

Isolation and Culture of Adult Mouse Hepatocytes

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 633))

Abstract

The liver performs a multitude of functions including the regulation of carbohydrate, fat, and protein metabolism, the detoxification of endo- and xenobiotics, and the synthesis and secretion of plasma proteins and bile. Isolated hepatocytes constitute a useful technique for studying liver function in an in vitro setting. Here we describe a method for the isolation of hepatocytes from adult mouse liver. The principle of the method is the two-step collagenase perfusion technique which involves sequential perfusion of the liver with ethylenediaminetetraacetic acid and collagenase. Following isolation, the cells can either be used for short-term studies or, alternatively, maintained in culture for prolonged periods to study long-term changes in gene expression. The protocol for mouse hepatocyte isolation may be applied to both normal and transgenic mice.

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Author information

Authors and Affiliations

  1. Department of Biology and Biochemistry, Centre for Regenerative Medicine, University of Bath, BA2 7AY, Bath, UK

    Wan-Chun Li, Kate L. Ralphs & David Tosh

Authors
  1. Wan-Chun Li
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  2. Kate L. Ralphs
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  3. David Tosh
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Editor information

Editors and Affiliations

  1. Dept. Biology & Biochemistry, University of Bath, Claverton Down, Bath, BA2 7AY, United Kingdom

    Andrew Ward

  2. Dept. Biology & Biochemistry, University of Bath, Claverton Down, Bath, BA2 7AY, United Kingdom

    David Tosh

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Li, WC., Ralphs, K.L., Tosh, D. (2010). Isolation and Culture of Adult Mouse Hepatocytes. In: Ward, A., Tosh, D. (eds) Mouse Cell Culture. Methods in Molecular Biology, vol 633. Humana Press. https://doi.org/10.1007/978-1-59745-019-5_13

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  • DOI: https://doi.org/10.1007/978-1-59745-019-5_13

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-772-3

  • Online ISBN: 978-1-59745-019-5

  • eBook Packages: Springer Protocols

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