Summary
There is an increasing awareness of the role of inflammation in cancer. Immune responses can limit the growth of some tumors, but paradoxically, may promote the growth of others. Cytokines are critical mediators of both the innate and the adaptive immune responses. In this chapter, we will describe several methods for the detection of inflammatory cytokines. First, we will describe a protocol for quantification of cytokine mRNA by real-time quantitative PCR. In addition, we will describe detection of cytokine proteins by ELISA as well as by novel cytokine bead arrays. Finally, a method will be described for in situ detection of cytokine production by immunohistochemistry.
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Acknowledgments
The authors would like to thank Dr. J. Magarian Blander for critical reading of the manuscript and help with the section on real-time PCR primer-probe sets. We further thank A. Antov for providing template real-time PCR graphs. T.T. is supported by an Alzheimer's Association grant and a National Institutes of Health “Pathway to Independence” award (1K99AG029726 and 4R00AG029726). D.A. is supported by an AMC fellowship and by an award from the Landsteiner Foundation. K.E.dV is supported by a grant from the Dutch Cancer Society (NKI 2006–3715). T.T. holds the Ben Winters Chair in Regenearative Medicine
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Amsen, D., de Visser, K.E., Town, T. (2009). Approaches to Determine Expression of Inflammatory Cytokines. In: Kozlov, S.V. (eds) Inflammation and Cancer. Methods in Molecular Biology™, vol 511. Humana Press. https://doi.org/10.1007/978-1-59745-447-6_5
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DOI: https://doi.org/10.1007/978-1-59745-447-6_5
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