Summary
Biosensors represent an interesting tool in the search of bioactive compounds. In particular, optical sensors based on Surface Plasmon Resonance transduction (SPR) allow monitoring of biomolecular interaction in real time and without any labelling of the interactants. The biosensor analysis can be applied to both pure compounds or to complex mixtures (e.g. plant extract). The SPR detection principle is here presented and the application to the analysis of plant extracts (i.e. of Chelidonium majus L.) as a paradigmatic example for the search of bioactive compounds able to interact with DNA, is also discussed.
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References
Dove, A. (1999) Drug screening - beyond the bottleneck. Nat. Biotechnol. 17, 859–863
Rademann, J. and Jung, G. (2000) Techview: drug discovery: integrating combinatorial synthesis and bioassays. Science 287, 1947–1948
Lee, T. R. and Lawrence, D. S. (2000) SH2-directed ligands of the Lck tyrosine kinase. J. Med. Chem. 43, 1173–1179
Campanella, L., Favero, G., Persi, L. and Tomassetti, M. (2000) New biosensor for superoxide radical used to evidence molecules of biomedical and pharmaceutical interest having radical scavenging properties. J. Pharm. Biomed. Anal. 23, 69–76
Danelian, E., Karlén, A., Karlsson, R., Winiwarter, S., Hansson, A., Löfas, S., Lennernäs, H. and Hämäläinen, M. (2000) SPR biosensor studies of the direct interaction between 27 drugs and a liposome surface: correlation with fraction absorbed in humans. J. Med. Chem. 3, 2083–2086
White, R. E. (2000) High-throughput screening in drug metabolism and pharmacokinetic support of drug discovery. Annu. Rev. Pharmacol. Toxicol. 40, 133–157
Turner, A. P. F., Karube, I. and Wilson G. S. (eds.) (1987) Biosensor – Fundamentals and Applications, 1st edn., Oxford University Press, UK
Tombelli, S., Minunni, M. and Mascini, M. (2007) Aptamers for diagnostics, environmental and food quality applications. Biomol. Eng. 24(2), 191–200
Minunni, M. and Bilia, A. (2007) Biosensing approach in natural products research. In: Colegate, S. M. and Molyneaux, R. J. (eds.) Bioactive Natural Products. Detection, Isolation and Structural Determination, 2nd edn, Chapter 11. CRC Press, Inc, Boca Raton, FL
Donghui, Y., Blankert, B., Vire, J. C. and Kauffmann, J. M. (2005) Biosensors in drug discovery and drug analysis. Anal. Lett. 38, 1687–1701
Cooper, M. A. (2002) Optical biosensors in drug discovery. Nat. Rev. Drug. Discov. 1, 515–528
Minunni, M., Tombelli, S., Mascini, M., Bilia, A. R., Bergonzi, M. C. and Vincieri, F. F. (2005) An optical DNA-based biosensor for the analysis of bioactive constituents with application in drug and herbal drug screening. Talanta 65, 578–585
Colombo, M. L. and Bosisio, E. (1996) Pharmacological activities of Chelidonium majus L. (Papaveraceae). Pharmacol. Res. 33, 127–134
Kokoska, L., Polesny, Z., Rada, V., Nepovim, A. and Vanek, T. (2002) Screening of some Siberian medicinal plants for antimicrobial activity. J. Ethnopharmacol. 82, 51–53
Kim, D. J., Ahn, B., Han, B. S. and Tsuda, H. (1997) Potential preventive effects of Chelidonium majis L. (Papaveraceae) herb extract on glandular stomach tumor development in rats treated with N-methyl-N′-nitro-N nitrosoguanidine (MNNG) and hypertonic sodium chloride. Cancer Lett. 112, 203–208
Fang, S., Lee, H. J., Wark, A. W. and Corn, R. (2006) Attomole Microarray detection of microRNAs by nanoparticle-amplified SPR Imaging measurements of surface polyadenylation reactions. J. Am. Chem. Soc. 128, 14044–14046
Kong, X. B., Rubin, L., Chen, L. I., Cuszewska,G., Watanabe K. A., Tong, W. P., Sirotnak, F. M. and Chou, T. C. (1992) Topoisomerase II-mediated DNA cleavage activity and irreversibility of cleavable complex formation induced by DNA intercalator with alkylating capability. Mol. Pharmacol. 41, 237–244
Das, S., Kumar, G. S. and Maiti, M. (1999) Conversions of the left-handed form and the protonated form of DNA back to the bound right-handed form by sanguinarine and ethidium: a comparative study. Biophys. Chem. 76, 199–218
Sen, A., Ray, A. and Maiti, M., (1996) Thermodynamics of the interactions of sanguinarine with DNA: influence of ionic strength and base composition. Biophys. Chem. 59, 155–170
Biacore, T. M. (1991) System manual, Uppsala, Sweden, Parmacia Biosensor AB
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Minunni, M., Bilia, A.R. (2010). SPR in Drug Discovery: Searching Bioactive Compounds in Plant Extracts. In: Roque, A. (eds) Ligand-Macromolecular Interactions in Drug Discovery. Methods in Molecular Biology, vol 572. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-244-5_13
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DOI: https://doi.org/10.1007/978-1-60761-244-5_13
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