Abstract
Regulatory macrophages (M regs) are a novel type of suppressor macrophage which may be a particularly suitable cell for inducing tolerance of solid organ transplants. In this article, we provide a detailed description of the generation of human M regs from peripheral blood monocytes and methods for the assessment of their phenotype. The uniqueness of the human M reg is best appreciated when the M reg is compared to macrophages in other states of activation; therefore, protocols are provided for generating five comparator macrophage types which have been used as cell type-specificity controls in our work.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Hutchinson JA, Govert F, Riquelme P et al. (2009) Administration of donor-derived transplant acceptance-inducing cells to the recipients of renal transplants from deceased donors is technically feasible. Clin Transplant 23: 140–145.
Hutchinson JA, Riquelme P, Brem-Exner BG et al. (2008) Transplant acceptance-inducing cells as an immune-conditioning therapy in renal transplantation. Transpl Int 21: 728–741.
Hutchinson JA, Brem-Exner BG, Riquelme P et al. (2008) A cell-based approach to the minimization of immunosuppression in renal transplantation. Transpl Int 21: 742–754.
Hutchinson JA, Roelen D, Riquelme P et al. (2008) Preoperative treatment of a presensitized kidney transplant recipient with donor-derived transplant acceptance-inducing cells. Transpl Int 21: 808–813.
Chatenoud L (2008) The long and winding road towards induction of allograft tolerance in the clinic. Transpl Int 21: 725–727.
Warnecke G, Hutchinson JA, Riquelme P et al. (2009) Postoperative intravenous infusion of donor-derived transplant acceptance-inducing cells as an adjunct immunosuppressive therapy in a porcine pulmonary allograft model. Transpl Int 22: 332–341.
Brem-Exner BG, Sattler C, Hutchinson JA (2008) Macrophages driven to a novel state of activation have anti-inflammatory properties in mice. J Immunol 180(1): 335–349.
Riquelme P, Govert F, Geissler EK et al. (2009) Human transplant acceptance-inducing cells suppress mitogen-stimulated T cell proliferation. Transpl Immunol 21(3): 162–165.
Riquelme P, Wundt J, Hutchinson JA et al. (2009) A refined characterisation of the NeoHepatocyte phenotype necessitates a reappraisal of the transdifferentiation hypothesis. Differentiation 77(3): 263–276.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2010 Humana Press
About this protocol
Cite this protocol
Hutchinson, J.A., Riquelme, P., Geissler, E.K., Fändrich, F. (2010). Human Regulatory Macrophages. In: Cuturi, M., Anegon, I. (eds) Suppression and Regulation of Immune Responses. Methods in Molecular Biology, vol 677. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-869-0_13
Download citation
DOI: https://doi.org/10.1007/978-1-60761-869-0_13
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-60761-868-3
Online ISBN: 978-1-60761-869-0
eBook Packages: Springer Protocols