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Blood-Brain Barrier Disruption in the Treatment of Brain Tumors

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The Blood-Brain and Other Neural Barriers

Part of the book series: Methods in Molecular Biology ((MIMB,volume 686))

Abstract

Standard chemotherapy administered systemically has a limited efficacy in the treatment of brain tumors. One of the major obstacles in the treatment of brain neoplasias is the impediment to delivery across the intact blood-brain barrier (BBB). Many innovative approaches have been developed to circumvent this obstacle. One such strategy is BBB disruption (BBBD), which successfully increases the delivery of antineoplastic agents to the central nervous system (CNS). This chapter describes the application of the BBBD technique in rats. Different methods to evaluate and measure BBB permeability following hyperosmolar mannitol infusion including Evans blue staining, albumin immunohistochemistry, and dynamic magnetic resonance imaging are also described.

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Acknowledgments

This work was supported by a grant from the CIHR (DF), as well as the National Bank of Canada Research Chair on Brain Tumor treatment (DF).

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Authors and Affiliations

  1. Departments of Neurosurgery and Neuro-oncology, University of Sherbrooke Hospital, Sherbrooke, QC, Canada

    Marie Blanchette & David Fortin

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  1. Marie Blanchette
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  2. David Fortin
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Editors and Affiliations

  1. Banting Institute, Dept. Laboratory Med. & Pathobiology, University of Toronto, College St. 100, Toronto, M5G 1L5, Ontario, Canada

    Sukriti Nag

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Blanchette, M., Fortin, D. (2011). Blood-Brain Barrier Disruption in the Treatment of Brain Tumors. In: Nag, S. (eds) The Blood-Brain and Other Neural Barriers. Methods in Molecular Biology, vol 686. Humana Press. https://doi.org/10.1007/978-1-60761-938-3_23

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  • DOI: https://doi.org/10.1007/978-1-60761-938-3_23

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-937-6

  • Online ISBN: 978-1-60761-938-3

  • eBook Packages: Springer Protocols

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