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Next-Generation MicroRNA Expression Profiling Technology

Methods and Protocols

  • Book
  • © 2012

Overview

Editors:
  1. Jian-Bing Fan

    1. Illumina, Inc., San Diego, USA

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  • Covers innovative technologies that are more sensitive, specific, and quantitative, as well as being compatible with a wide range of biospecimens
  • Features tried-and-true lab protocols that are ready to be put into action
  • Includes expert tips and implementation advice to ensure successful results

Part of the book series: Methods in Molecular Biology (MIMB, volume 822)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Controlling miRNA Regulation in Disease

    • Willemijn M. Gommans, Eugene Berezikov
    Pages 1-18

  3. Introduction to miRNA Profiling Technologies and Cross-Platform Comparison

    • Sarah Aldridge, James Hadfield
    Pages 19-31

  4. Stem-Loop RT-qPCR for MicroRNA Expression Profiling

    • James Hurley, Doug Roberts, Andrew Bond, David Keys, Caifu Chen
    Pages 33-52

  5. Poly(T) Adaptor RT-PCR

    • Rui Shi, Ying-Husan Sun, Xing-Hai Zhang, Vincent L. Chiang
    Pages 53-66

  6. MicroRNA In Situ Hybridization

    • Boye Schnack Nielsen
    Pages 67-84

  7. Agilent MicroRNA Microarray Profiling System

    • Petula N. D’Andrade, Stephanie Fulmer-Smentek
    Pages 85-102

  8. miRNA Expression Profiling Using Illumina Universal BeadChips

    • Jing Chen, Craig S. April, Jian-Bing Fan
    Pages 103-116

  9. MicroRNA Expression Analysis Using the Affymetrix Platform

    • Suzanne Dee, Robert C. Getts
    Pages 117-129

  10. Individualized miRNA Assay Panels Using Optically Encoded Beads

    • Keld Sorensen
    Pages 131-141

  11. Microfluidic Primer Extension Assay

    • Markus Beier, Valesca Boisguérin
    Pages 143-152

  12. MicroRNA Profiling Using µParaflo Microfluidic Array Technology

    • Xiaochuan Zhou, Qi Zhu, Christoph Eicken, Nijing Sheng, Xiaolin Zhang, Litao Yang et al.
    Pages 153-182

  13. MicroRNA Expression Analysis Using the Illumina MicroRNA-Seq Platform

    • Shujun Luo
    Pages 183-188

  14. Next-Generation Sequencing of miRNAs with Roche 454 GS-FLX Technology: Steps for a Successful Application

    • Ana Raquel Soares, Patrícia M. Pereira, Manuel A. S. Santos
    Pages 189-204

  15. Methods for Small RNA Preparation for Digital Gene Expression Profiling by Next-Generation Sequencing

    • Sam E. V. Linsen, Edwin Cuppen
    Pages 205-217

  16. Profiling of Short RNAs Using Helicos Single-Molecule Sequencing

    • Philipp Kapranov, Fatih Ozsolak, Patrice M. Milos
    Pages 219-232

  17. deepBase: Annotation and Discovery of MicroRNAs and Other Noncoding RNAs from Deep-Sequencing Data

    • Jian-Hua Yang, Liang-Hu Qu
    Pages 233-248

  18. PhenomiR: MicroRNAs in Human Diseases and Biological Processes

    • Andreas Ruepp, Andreas Kowarsch, Fabian Theis
    Pages 249-260

  19. miRNA Expression Profiling: From Reference Genes to Global Mean Normalization

    • Barbara D’haene, Pieter Mestdagh, Jan Hellemans, Jo Vandesompele
    Pages 261-272

  20. miRNA Data Analysis: Next-Gen Sequencing

    • Preethi H. Gunaratne, Cristian Coarfa, Benjamin Soibam, Arpit Tandon
    Pages 273-288
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Keywords

About this book

The rapid pace of microRNA (miRNA) research continues to drive the advances of techniques for miRNA expression profiling, and innovative technologies that are more sensitive, specific, quantitative, and that are compatible with a wide range of biospecimens have been developed during the past few years.  In Next-Generation MicroRNA Expression Profiling Technology: Methods and Protocols, expert researchers in the field contribute detailed examinations of the most current approaches being used today.  This volume includes comprehensive coverage of methodologies that have been developed for miRNA profiling, as well as next-gen sequencing technology, miRNA databases, and specialized applications, such as cancer studies and miRNA-based non-invasive biomarker development.  Written in the highly successful Methods in Molecular Biology™ series format, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Next-Generation MicroRNA Expression Profiling Technology: Methods and Protocols provides an ideal guide for novices and experts alike who are seeking to utilize these powerful technologies.

Editors and Affiliations

  • Illumina, Inc., San Diego, USA

    Jian-Bing Fan

Bibliographic Information

  • Book Title: Next-Generation MicroRNA Expression Profiling Technology

  • Book Subtitle: Methods and Protocols

  • Editors: Jian-Bing Fan

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-427-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-426-1Published: 06 December 2011

  • Softcover ISBN: 978-1-4939-6217-4Published: 23 August 2016

  • eBook ISBN: 978-1-61779-427-8Published: 07 December 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 341

  • Topics: Human Genetics, Gene Expression

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