Abstract
It is now recognized that the cell-to-cell transmission of misfolded proteins such as α-synuclein contributes to the neurodegenerative phenotype in neurological disorders such as idiopathic Parkinson’s disease, Dementia with Lewy bodies, and Parkinson’s disease dementia. Thus, establishing cell-based models for the transmission of α-synuclein is of importance to understand the mechanisms of neurodegeneration in these disorders and to develop new therapies. Here we describe methods to study the neuron-to-neuron propagation of α-synuclein in an in vitro setting that also has in vivo applications.
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Acknowledgments
Dr. EM work was supported by NIH grants AG18440, AG022074, NS057096, AG 03097, and AG010435. Dr. SJL work was supported by Mid-career Researcher Program (2010–0015188) and the Diseases Network Research Program (2010–0020610) through NRF grant funded by the Ministry of Education, Science, and Technology.
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Lee, SJ., Desplats, P., Lee, HJ., Spencer, B., Masliah, E. (2012). Cell-to-Cell Transmission of α-Synuclein Aggregates. In: Sigurdsson, E., Calero, M., Gasset, M. (eds) Amyloid Proteins. Methods in Molecular Biology, vol 849. Humana Press. https://doi.org/10.1007/978-1-61779-551-0_23
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DOI: https://doi.org/10.1007/978-1-61779-551-0_23
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