Abstract
Luminescent-conjugated oligo- and polythiophenes (LCOs and LCPs) are valuable tools for optical imaging of a plethora of protein aggregates associated with amyloidoses. Here, we describe the utilization of an anionic pentameric LCO, p-FTAA, for staining of protein aggregates in a variety of platforms, including in vitro-formed amyloid fibrils and tissue sections.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Westermark, P., Benson, M. D., Buxbaum, J. N., Cohen, A. S., Frangione, B., Ikeda, S., Masters, C. L., Merlini, G., Saraiva, M. J., and Sipe, J. D. (2007) A primer of amyloid nomenclature. Amyloid 14, 179–183.
Bennhold H. (1922) Eine specifische Amyloidfärbung mit Kongorot. Műnchener Med. Wochenschrift 44, 1537–1538.
Divry P. (1927) Etude histochimique des plaques seniles. J. Neurol. Psychiatry 27, 643–657.
Nilsson, K. P. R., Herland, A., Hammarström, P., and Inganäs, O. (2005) Conjugated polyelectrolytes – conformation sensitive optical probes for detection of amyloid fibril formation. Biochemistry 44, 3718–3724.
Nilsson, K. P. R., Hammarström, P., Ahlgren, F., Herland, A., Schnell, E. A., Lindgren, M., Westermark, G. T., and Inganäs, O. (2006) Conjugated polyelectrolytes - Conformation-sensitive optical probes for staining and characterization of amyloid deposits. Chembiochem. 7, 1096–1104.
Nilsson, K. P. R., Åslund, A., Berg, I., Nyström, S., Konradsson, P., Inganäs, O., Stabo-Eeg, F., Lindgren, M., Westermark, G. T., Lannfelt, L., Nilsson, L. N., and Hammarström, P. (2007) Imaging distinct conformational states of amyloid-beta fibrils in Alzheimer’s disease using novel luminescent probes. ACS Chem. Biol. 2, 553–560.
Sigurdson, C. J., Nilsson, K. P. R., Hornemann, S., Manco, G., Polymenidou, M., Schwarz, P., Leclerc, M., Hammarström, P, Wüthrich, K., and Aguzzi, A. (2007) Prion strain discrimination using luminescent conjugated polymers. Nature Methods 12, 1023–1030.
Sigurdson, C. J., Nilsson, K. P. R., Hornemann, S., Heikenwalder, M., Manco, G., Schwarz, P., Ott, D., Rülicke, T., Liberski, P. P., Julius, C., Falsig, J., Stitz, L., Wüthrich, K., and Aguzzi, A. (2009) De novo generation of a transmissible spongiform encephalopathy by mouse transgenesis. Proc. Natl. Acad. Sci. USA 106, 304–309.
Nilsson, K. P. R. (2009) Small organic probes as amyloid specific ligands - Past and recent molecular scaffolds. FEBS Lett. 583, 2593–2599.
Åslund, A., Sigurdson, C. J., Klingstedt, T., Grathwohl, S., Bolmont, T., Dickstein, D. L., Glimsdal, E., Prokop, S., Lindgren, M., Konradsson, P., Holtzman, D. M., Hof, P. R., Heppner, F. L., Gandy, S., Jucker, M., Aguzzi, A., Hammarström, P., and Nilsson, K. P. R. (2009) Novel pentameric thiophene derivatives for in vitro and in vivo optical imaging of a plethora of protein aggregates in cerebral amyloidoses. ACS Chem. Biol. 4, 673–684.
Nilsson, K. P. R., Ikenberg, K., Aslund, Å, Fransson, S., Konradsson, P., Röcken, C., Moch, H., and Aguzzi, A. (2010) Structural typing of systemic amyloidoses by luminescent-conjugated polymer spectroscopy. Am. J. Pathol. 176, 563–574.
Berg, I., Nilsson, K. P. R., Thor, S., and Hammarström, P. (2010) Efficient imaging of amyloid deposits in Drosophila models of human amyloidoses. Nature Protocols 5, 935–944.
Hammarström, P., Simon, R., Nyström, S., Konradsson, P., Åslund, A., and Nilsson, K. P. R. (2010) A fluorescent pentameric thiophene derivative detects in vitro-formed prefibrillar protein aggregates. Biochemistry 49, 6838–6845.
Nilsson, K. P. R., Joshi-Barr, S., Winson, O., and Sigurdson, C. J. (2010) Prion strain interactions are highly selective. J Neurosci. 30, 12094–12102.
Stabo-Eeg, F., Lindgren, M., Nilsson, K. P. R., Inganäs, O., and Hammarström, P. (2007) Quantum efficiency and two-photon absorption cross-section of conjugated polyelectrolytes used for protein conformation measurements with applications on amyloid structures. Chemical Physics 336, 121–126.
Lindgren, M., Glimsdal, E., Åslund, A., Simon, R., Hammarström, P., Nilsson, K. P. R. (2010) Luminescence and Two-Photon Absorption Cross Section of Novel Oligomeric Luminescent Conjugated Polythiophenes for Diagnostics of Amyloid Fibrils. Nonlinear Optics, Quantum Optics: concepts in modern optics 40, 241–251.
Acknowledgments
This work was supported by the Swedish Research Council (PH), Knut and Alice Wallenberg Foundation (PH, KPRN), Linköping University (PH, KPRN), The Swedish Foundation for Strategic Research (PH, KPRN), The European Union FP7 HEALTH (Project LUPAS) (KPRN, PH, ML), and The European Research Council (ERC, Project MUMID) (KPRN).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Nilsson, K.P.R., Lindgren, M., Hammarström, P. (2012). A Pentameric Luminescent-Conjugated Oligothiophene for Optical Imaging of In Vitro-Formed Amyloid Fibrils and Protein Aggregates in Tissue Sections. In: Sigurdsson, E., Calero, M., Gasset, M. (eds) Amyloid Proteins. Methods in Molecular Biology, vol 849. Humana Press. https://doi.org/10.1007/978-1-61779-551-0_29
Download citation
DOI: https://doi.org/10.1007/978-1-61779-551-0_29
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-61779-550-3
Online ISBN: 978-1-61779-551-0
eBook Packages: Springer Protocols