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Odontogenesis pp 41–47Cite as

Detection of Apoptosis by TUNEL Assay

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 887))

Abstract

Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay has been designed to detect apoptotic cells that undergo extensive DNA degradation during the late stages of apoptosis. The method is based on the ability of TdT to label blunt ends of double-stranded DNA breaks independent of a template. This chapter describes an assay for detection of apoptotic cells during mouse odontogenesis using a colorimetric TUNEL system.

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Author information

Authors and Affiliations

  1. Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, OR, USA

    Kateryna Kyrylkova, Sergiy Kyryachenko & Mark Leid

  2. College of Pharmacy, Department of Pharmaceutical Sciences, Oregon State University, Corvallis, OR, USA

    Chrissa Kioussi

Authors
  1. Kateryna Kyrylkova
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  2. Sergiy Kyryachenko
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  3. Mark Leid
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  4. Chrissa Kioussi
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Corresponding author

Correspondence to Chrissa Kioussi .

Editor information

Editors and Affiliations

  1. College of Pharmacy, Dept. of Pharmaceutical Sciences, Oregon State University, SW Jefferson Street 1600, Corvallis, 97333, Oregon, USA

    Chrissa Kioussi

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© 2012 Springer Science+Business Media, LLC

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Kyrylkova, K., Kyryachenko, S., Leid, M., Kioussi, C. (2012). Detection of Apoptosis by TUNEL Assay. In: Kioussi, C. (eds) Odontogenesis. Methods in Molecular Biology, vol 887. Humana Press. https://doi.org/10.1007/978-1-61779-860-3_5

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  • DOI: https://doi.org/10.1007/978-1-61779-860-3_5

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-859-7

  • Online ISBN: 978-1-61779-860-3

  • eBook Packages: Springer Protocols

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