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Spermatogenesis pp 257–262Cite as

Sperm Selection for ICSI Using Annexin V

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 927))

Abstract

Annexin-V magnetic-activated cell sorting (MACS) is a new tool to optimize sperm selection in assisted reproduction. The technique is based on the binding of superparamagnetic Annexin-microbeads to externalized phosphatidylserine at the outer leaflet of the plasma membrane of sperm with activated apoptosis signaling or membrane damage. The combination of Annexin-V MACS and density gradient centrifugation was demonstrated to enhance clinical pregnancy rates in ICSI cycles. This chapter focuses on the practical details of Annexin-V MACS.

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Author information

Authors and Affiliations

  1. EAA Training Center, Klinik und Poliklinik für Dermatologie, Venerologie und Allergologie, Universitätsklinikum Leipzig AöR, Leipzig, Germany

    Sonja Grunewald & Uwe Paasch

Authors
  1. Sonja Grunewald
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  2. Uwe Paasch
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Corresponding author

Correspondence to Uwe Paasch .

Editor information

Editors and Affiliations

  1. Andrology and IVF Laboratories, Department of Surgery (Urology), University of Utah School of Medicine, Arapeen Dr. 675, Salt Lake City, 84108, Utah, USA

    Douglas T. Carrell

  2. , Andrology and IVF Laboratories, University of Utah School of Medicine, Arapeen Way 675, Salt Lake City, 84108, Utah, USA

    Kenneth I. Aston

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Grunewald, S., Paasch, U. (2013). Sperm Selection for ICSI Using Annexin V. In: Carrell, D., Aston, K. (eds) Spermatogenesis. Methods in Molecular Biology, vol 927. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-038-0_23

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  • DOI: https://doi.org/10.1007/978-1-62703-038-0_23

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-037-3

  • Online ISBN: 978-1-62703-038-0

  • eBook Packages: Springer Protocols

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