Abstract
Microsatellites are ubiquitous throughout eukaryotic genomes and are useful in analyzing populations and genetic diversity. The QIAxcel system, an automated capillary electrophoresis device, allows the user to determine the size of microsatellite fragments, to discern allelic polymorphisms among individuals, and to differentiate homozygous and heterozygous individuals. This system provides comparable base pair resolution to more expensive systems at a relatively affordable cost.
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Gupta PK, Varshney RK (2000) The development and use of microsatellite markers for genetics and plant breeding with emphasis on bread wheat. Euphytica 113:163–185
Wadl PA, Wang X, Moulton JK, Hokanson SC, Skinner JA, Rinehart TA, Reed SM, Pantalone VR, Trigiano RN (2010) Transfer of Cornus florida and C. kousa simple sequence repeats to selected Cornus (Cornaceae) species. J Am Soc Hort Sci 135:279–288
Wang X, Rinehart TA, Wadl PA, Spiers JM, Hadziabdic D, Windham MT, Trigiano RN (2009) A new electrophoresis technique to separate microsatellite alleles. Afr J Biotechnol 8:2432–2436
Hadziabdic D, Wang X, Wadl PA, Rinehart TA, Ownley BH, Trigiano RN (2012) Genetic diversity of flowering dogwood in the Great Smoky Mountains National Park. Tree Genet Genomes. doi:10.1007/s11295-012-0471-1
Dean D, Wadl PA, Wang X, Klingeman WE, Ownley BH, Rinehart TA, Scheffler BE, Trigiano RN (2011) Screening and characterization of 11 novel microsatellite markers from Viburnum dilatatum. HortScience 46:1456–1459
QIAGEN (2011) QIAxcel® DNA handbook. 2nd edn. http://www.QIAGEN.com/literature/handbooks/literature.aspx?id=2000066. Accessed 03 Feb 2012
QIAGEN (2008) User manual. http://www.qiagen.com/products/qiaxcelsystem.aspx#Tabs=t5. Accessed 03 Feb 2012
Gupta V, Dorsey G, Hubbard A, Rosenthal PJ, Greenhouse B (2010) Gel versus capillary electrophoresis genotyping for categorizing treatment outcomes in two anti-malarial trials in Uganda. Malaria J. 9. http://malariajourna.com/content/9/1/19. Accessed 12 Feb 2012
Amos W, Hoffmann JI, Frodsham A, Zhang L, Best S, Hill AVS (2007) Automated binning of microsatellite alleles: problems and solutions. Mol Ecol Notes 7:10–14
Jakubauskas A, Griskevicius L (2010) KRas and BRaf mutational status analysis from formalin-fixed, paraffin-embedded tissues using multiplex polymerase chain reaction-based assay. Arch Pathol Lab Med 134:620–624
Salgotra RM, Millwood RJ, Agarwal S, Stewart N (2011) High-throughput functional marker assay for detection of Xa/xa and fgr genes in rice (Oryza sativa L.). Electrophoresis 32:2216–2222
Chamberlain JS, Gibbs RA, Ranier JE, Nguyen PN, Caskey CT (1988) Deletion screening of the Duchenne muscular dystrophy locus via multiplex DNA amplification. Nucleic Acids Res 16:11141–11156
Korbie DJ, Mattick JS (2008) Touchdown PCR for increased specificity and sensitivity in PCR amplification. Nat Protoc 3:1452–1456
Trigiano RN, Wadl PA, Dean D, Hadziabdic D, Scheffler BE, Runge F, Telle S, Thines M, Ristaino J, Spring O (2012) Ten polymorphic microsatellite loci identified from a small insert genomic library for Peronospora tabacina. Mycologia. doi:10.3852/11-288
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Dean, D.A., Wadl, P.A., Hadziabdic, D., Wang, X., Trigiano, R.N. (2013). Analyzing Microsatellites Using the QIAxcel System. In: Kantartzi, S. (eds) Microsatellites. Methods in Molecular Biology, vol 1006. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-389-3_16
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DOI: https://doi.org/10.1007/978-1-62703-389-3_16
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