Abstract
The generation of transgenic mouse models has been a powerful technique for several decades and is still widely used. There have been many manuals and general reviews of this technology. This chapter is designed to be a “how-to” resource with detailed specifics.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Jaenisch R, Mintz B (1974) Simian virus 40 DNA sequences in DNA of healthy adult mice derived from preimplantation blastocysts injected with viral DNA. Proc Natl Acad Sci USA 71(4):1250–1254
Gordon JW, Scangos GA, Plotkin DJ, Barbosa JA, Ruddle FH (1980) Genetic transformation of mouse embryos by microinjection of purified DNA. Proc Natl Acad Sci USA 77(12):7380–7384
Brinster RL, Chen HY, Trumbauer M, Senear AW, Warren R, Palmiter RD (1981) Somatic expression of herpes thymidine kinase in mice following injection of a fusion gene into eggs. Cell 27(1 Pt 2):223–231
Costantini F, Lacy E (1981) Introduction of a rabbit beta-globin gene into the mouse germ line. Nature 294(5836):92–94
Wagner EF, Stewart TA, Mintz B (1981) The human beta-globin gene and a functional viral thymidine kinase gene in developing mice. Proc Natl Acad Sci USA 78(8):5016–5020
Palmiter RD, Brinster RL (1986) Germ-line transformation of mice. Annu Rev Genet 20:465–499
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Skelton, J., Gower, L.H., McGough, A., Labosky, P.A. (2013). Transgenic Mouse Models. In: Allen, I. (eds) Mouse Models of Allergic Disease. Methods in Molecular Biology, vol 1032. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-496-8_1
Download citation
DOI: https://doi.org/10.1007/978-1-62703-496-8_1
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-495-1
Online ISBN: 978-1-62703-496-8
eBook Packages: Springer Protocols