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Whole Tooth Regeneration as a Future Dental Treatment

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Book cover Engineering Mineralized and Load Bearing Tissues

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 881))

Abstract

Dental problems caused by dental caries, periodontal disease and tooth injury compromise the oral and general health issues. Current advances for the development of regenerative therapy have been influenced by our understanding of embryonic development, stem cell biology, and tissue engineering technology. Tooth regenerative therapy for tooth tissue repair and whole tooth replacement is currently expected a novel therapeutic concept with the full recovery of tooth physiological functions. Dental stem cells and cell-activating cytokines are thought to be candidate approach for tooth tissue regeneration because they have the potential to differentiate into tooth tissues in vitro and in vivo. Whole tooth replacement therapy is considered to be an attractive concept for next generation regenerative therapy as a form of bioengineered organ replacement. For realization of whole tooth regeneration, we have developed a novel three-dimensional cell manipulation method designated the “organ germ method”. This method involves compartmentalisation of epithelial and mesenchymal cells at a high cell density to mimic multicellular assembly conditions and epithelial-mesenchymal interactions in organogenesis. The bioengineered tooth germ generates a structurally correct tooth in vitro, and erupted successfully with correct tooth structure when transplanted into the oral cavity. We have ectopically generated a bioengineered tooth unit composed of a mature tooth, periodontal ligament and alveolar bone, and that tooth unit was engrafted into an adult jawbone through bone integration. Bioengineered teeth were also able to perform physiological tooth functions such as mastication, periodontal ligament function and response to noxious stimuli. In this review, we describe recent findings and technologies underpinning whole tooth regenerative therapy.

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Acknowledgements

This work was partially supported by Health and Labour Sciences Research Grants from the Ministry of Health, Labour, and Welfare (no. 21040101) to Akira Yamaguchi (Tokyo Medical and Dental University), a Grant-in-Aid for Scientific Research (A) (no. 20249078) to T. Tsuji (2008–2010) and a Grant-in-Aid for Young Scientists (B) to M. Oshima from the Ministry of Education, Culture, Sports and Technology, Japan. This work was also partially supported by Organ Technologies Inc.

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Authors and Affiliations

  1. Department of Oral Rehabilitation and Regenerative Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, 700-8525, Japan

    Masamitsu Oshima Ph.D.

  2. RIKEN Center for Developmental Biology, Kobe, Hyogo, 650-0047, Japan

    Masamitsu Oshima Ph.D. & Takashi Tsuji Ph.D.

  3. Research Institute for Science and Technology, Tokyo University of Science, Noda, Chiba, 278-8510, Japan

    Takashi Tsuji Ph.D.

  4. Organ Technologies Inc, Tokyo, 101-0048, Japan

    Takashi Tsuji Ph.D.

Authors
  1. Masamitsu Oshima Ph.D.
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  2. Takashi Tsuji Ph.D.
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Correspondence to Takashi Tsuji Ph.D. .

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Editors and Affiliations

  1. Center for Regenerative Medicine, Oregon Health and Science University, Portland, Oregon, USA

    Luiz E. Bertassoni

  2. Department of Biomaterials and Biomimetics, New York University, New York, New York, USA

    Paulo G. Coelho

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Oshima, M., Tsuji, T. (2015). Whole Tooth Regeneration as a Future Dental Treatment. In: Bertassoni, L., Coelho, P. (eds) Engineering Mineralized and Load Bearing Tissues. Advances in Experimental Medicine and Biology, vol 881. Springer, Cham. https://doi.org/10.1007/978-3-319-22345-2_14

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