Skip to main content
SpringerLink
Log in
Book cover

Histopathologie der Haut pp 37–49Cite as

Immunfluoreszenztechniken

  • Chapter
  • First Online:

Part of the book series: Springer Reference Medizin ((SRM))

Zusammenfassung

Der immunfluoreszenzoptische Nachweis von Autoantikörpern hat für die Diagnostik blasenbildender Autoimmunerkrankungen eine zentrale Bedeutung. Auch bei anderen Erkrankungen, wie Epidermolysis bullosa hereditaria, Lupus erythematodes, leukozytoklastischer Vaskulitis und dem Lichen ruber stellt die Immunfluoreszenzmikroskopie einen wichtigen diagnostischen Baustein dar.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   589.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD   599.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Weiterführende Literatur

  • Fine JD, Eady RA, Bauer EA, Bauer JW, Bruckner-Tuderman L, Heagerty A, Hintner H, Hovnanian A, Jonkman MF, Leigh I et al (2008) The classification of inherited epidermolysis bullosa (EB): report of the third international consensus meeting on diagnosis and classification of EB. J Am Acad Dermatol 58:931–950

    Article  PubMed  Google Scholar 

  • Gammon WR, Briggaman RA, Inman AO 3rd, Queen LL, Wheeler CE (1984) Differentiating anti-lamina lucida and anti-sublamina densa anti-BMZ antibodies by indirect immunofluorescence on 1.0 M sodium chloride-separated skin. J Invest Dermatol 82:139–144

    Article  CAS  PubMed  Google Scholar 

  • Gammon WR, Kowalewski C, Chorzelski TP, Kumar V, Briggaman RA, Beutner EH (1990) Direct immunofluorescence studies of sodium chloride-separated skin in the differential diagnosis of bullous pemphigoid and epidermolysis bullosa acquisita. J Am Acad Dermatol 22:664–670

    Article  CAS  PubMed  Google Scholar 

  • Groves R, Schmidt E (2015) Immunobullous diseases. In: Griffiths C, Barker J, Bleiker T, Chalmers R, Creamer D (Hrsg) Rook’s textbook of dermatology, 9. Aufl. Wiley-Blackwell (im Druck)

    Google Scholar 

  • Hintner H, Stingl G, Schuler G, Fritsch P, Stanley J, Katz S, Wolff K (1981) Immunofluorescence mapping of antigenic determinants within the dermal-epidermal junction in the mechanobullous diseases. J Invest Dermatol 76:113–118

    Article  CAS  PubMed  Google Scholar 

  • Kneisel A, Hertl M (2011) Bullöse Autoimmundermatosen. Teil 2: Diagnose und Therapie. J Dtsch Dermatol Ges 9:927–947

    PubMed  Google Scholar 

  • Mutasim DF, Adams BB (2001) Immunofluorescence in dermatology. J Am Acad Dermatol 45:803–822

    Article  CAS  PubMed  Google Scholar 

  • Pohla-Gubo G, Kraus L, Hintner H (2011) Role of immunofluorescence microscopy in dermatology. G Ital Dermatol Venereol 146:127–142

    CAS  PubMed  Google Scholar 

  • Rose C, Bröcker EB, Zillikens D (2010) Clinical, histological and immunpathological findings in 32 patients with dermatitis herpetiformis Duhring. J Dtsch Dermatol Ges 8:265–270

    Article  PubMed  Google Scholar 

  • Schmidt E, Zillikens D (2010) Modern diagnosis of autoimmune blistering skin diseases. Autoimmun Rev 10:84–89

    Article  CAS  PubMed  Google Scholar 

  • Schmidt E, Zillikens D (2011) The diagnosis and treatment of autoimmune blistering skin diseases. Dtsch Arztebl Int 108:399–405

    PubMed  PubMed Central  Google Scholar 

  • Schmidt E, Zillikens D (2013) Pemphigoid diseases. Lancet 381:320–332

    Article  PubMed  Google Scholar 

  • Schulze F, Kasperkiewicz M, Zillikens D, Schmidt E (2013). Bullöses Pemphigoid. Hautarzt, 64:931–945

    Google Scholar 

  • van Beek N, Rentzsch K, Probst C, Komorowski L, Kasperkiewicz M, Fechner K, Blocker IM, Zillikens D, Stocker W, Schmidt E (2012) Serological diagnosis of autoimmune bullous skin diseases: prospective comparison of the BIOCHIP mosaic-based indirect immunofluorescence technique with the conventional multi-step single test strategy. Orphanet J Rare Dis 7:49

    Article  PubMed  PubMed Central  Google Scholar 

  • Vaughn Jones SA, Palmer I, Bhogal BS, Eady RA, Black MM (1995) The use of Michel’s transport medium for immunofluorescence and immunoelectron microscopy in autoimmune bullous diseases. J Cutan Pathol 22:365–370

    Article  CAS  PubMed  Google Scholar 

  • Vodegel RM, Jonkman MF, Pas HH, de Jong MC (2004) U-serrated immunodeposition pattern differentiates type VII collagen targeting bullous diseases from other subepidermal bullous autoimmune diseases. Br J Dermatol 151:112–118

    Article  CAS  PubMed  Google Scholar 

  • Zillikens D (2012) Bullöse Autoimmundermatosen. In: Plewig G, Landthaler M, Burgdorf WHC, Hertl M, Ruzicka T (Hrsg) Braun-Falco’s Dermatologie, Venerologie und Allergologie, 6. Aufl. Springer, Berlin, S 777–811

    Chapter  Google Scholar 

  • Zimmermann J, Bahmer F, Rose C, Zillikens D, Schmidt E (2010) Clinical and immunopathological spectrum of paraneoplastic pemphigus. J Dtsch Dermatol Ges 8:598–605

    PubMed  Google Scholar 

Download references

Danksagung

Wir danken Inge Atefi, Dr. Franziska Hübner und Dr. Artem Vorobyev für Hilfe bei der Erstellung der Immunfluoreszenzabbildungen.

Author information

Authors and Affiliations

  1. Klinik für Dermatologie, Allergologie und Venerologie, Universität zu Lübeck, Ratzeburger Allee 160, 23562, Lübeck, Deutschland

    E. Schmidt & D. Zillikens

Authors
  1. E. Schmidt
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. Zillikens
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to E. Schmidt .

Editor information

Editors and Affiliations

  1. Universitätsklinik für Dermatologie, Medizinische Universität Graz, Graz, Austria

    Lorenzo Cerroni

  2. Universitäts-Hautklinik, Eberhard-Karls-Universität, Tübingen, Germany

    Claus Garbe

  3. Universitäts-Hautklinik Münster, Münster, Germany

    Dieter Metze

  4. Dermatopathologie Friedrichshafen, Friedrichshafen, Germany

    Heinz Kutzner

  5. Universitätsklinik für Dermatologie und, Medizinische Universität Graz, Graz, Steiermark, Austria

    Helmut Kerl

Rights and permissions

Reprints and permissions

Copyright information

© 2016 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Schmidt, E., Zillikens, D. (2016). Immunfluoreszenztechniken. In: Cerroni, L., Garbe, C., Metze, D., Kutzner, H., Kerl, H. (eds) Histopathologie der Haut. Springer Reference Medizin. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-45133-5_3

Download citation

  • DOI: https://doi.org/10.1007/978-3-662-45133-5_3

  • Published:

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-45132-8

  • Online ISBN: 978-3-662-45133-5

  • eBook Packages: Medicine (German Language)

Publish with us

Policies and ethics

Search

Navigation