Abstract
• Background: This study was carried out in order to improve the understanding of the pathogenesis of uveitic glaucoma • Methods: Uveitis was induced in 48 Lewis rats by S-antigen injection. Intraocular pressure (10P) was measured by Tono-Pen-2 daily for 24 days in 16 animals. Histopathology was performed sequentially in 14 rats on days 3, 5, 7, 9, 12, 15 and 18 after S-antigen injection. Aqueous dynamics studies were performed on days 0, 3, 7, 14 and 21. Aqueous humor production was measured using an FIT-Calbumin dilution technique; outflow facility was measured using anterior chamber infusion with constant pressure • Results: IOP decreased to a mean of 16.5 +-4.3 mmHg from days 2-5 after S-antigen injections from a mean pre-experimental value of 20.5 +-5.4 mmHg. IOP increased from days 6 to 20 (35.8+-9.1 mmHg; P=0.00001). Histopathologic study revealed inflammation of the anterior and posterior segments from days 9 to 21 after S-antigen injection. Aqueous humor production decreased and outflow facility increased at day 3 after S-antigen injection. At days 7 and 14 after S-antigen injection, acqueous humor production was increased while outflow facility remained normal or was decreased • Conclusion: This model of uveitis glaucoma is characterized by three overlapping phases: (1) ocular hypertension, (2) ocular hypertension associated with clinical and histologic inflammation and (3) anatomic sequelae of uveitis and variable IOP. This model permits in vivo studies of mechanisms of IOP change associated with uveiteis.
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References
Beitch BR, Eakins KE (1969) The effects of prostaglandins on the intraocular pressure of the rabbit. Br J Pharmacol 37:158
Bito LZ, Camras CB, Gum GG, Resul B (1989) The ocular hypotensive effects and side effects of prostaglandins on the eyes of experimental animals. In: Bito LZ, Stjernschants J (eds) The ocular effects of prostaglandins and other eicosanoids. Liss, New York, pp 349–368
Brubaker RF (1989) Fluorophotometric studies of prostaglandin effects on the human eye: the lack of association of reduced intraocular pressure with altered flow or barrier function. In: Bito LZ, Stjernschants J (eds) The ocular effects of prostaglandins and other eicosanoids. Liss, New York, pp 477–481
Cantor LB, Schlaegel TFJr, Abrams JH, Weber JC (1992) Intraocular pressure in anterior uveitis. Glaucoma 14:155
Dorey C, Cozette J, Faure JP (1982) A simple and rapid method for isolation of retinal S antigen. Ophthalmic Res 14:249
Epstein DL, Hashimoto JM, Grant WM (1978) Serum obstruction of aqueous outflow in enucleated eyes. Am J Ophthalmol 86:101
Faure JP (1988) Autoimmunity and the retina. Curr Top Eye Res 2:215
Gery I, Mochizuki M, Nussenblatt RB (1986) Retinal specific antigens and immunopathogenic processes they provoke. Prog Ret Res 5:75
Hall AJH, Greenwood J, Patel BCK, Towler HMA, Lightman SL (1992) The effect of cytokines on the bloodretinal barrier of the anaesthetized rat. Invest Opthalmol Vis Sci 33:852
Kass MA, Podos SM, Moses RA, Becker B (1972) Prostaglandin El and aqueous humor dynamics. Invest Ophthalmol 11:1022
Kerstetter JR, Brubaker RF, Wilson SE, Kullerstrand LV (1988) Prostaglandin F2-l-isopropylester lowers intraocular pressure without decreasing aqueous humor flow. Am J Ophthalmol 105:30
Krupin T, Dorfman NH, Spector SM, Wax MB (1988) Secondary glaucoma associated with uveitis. Glaucoma 10:85
Mermoud A, Baerveldt G, Minckler DS, Lee MB, Rao NA (1994) Intraocular pressure in Lewis rats. Invest Ophthalmol Vis Sciε:ε
Nakajima M, Goh Y, Azuma I, Hayaishi O (1991) Effects of prostaglandin D2 and its analogue, BW245C, on intraocular pressure in human. Graefe's Arch Clin Exp Ophthalmol 229:411
NilssonSFE, Sperber GO, Bill A (1989) The effect of prostaglandin F21-isopropylester (PGF2-1E) on uveoscleral outflow. In: Bito LZ, Stjernschants J (eds) The ocular effects of prostaglandins and other eicosanoids. Liss, New York, pp 429–436
Nussenblatt RB (1991) Proctor lecture. Experimental autoimmune uveitis. Mechanisms of disease and clinical therapeutic indications. Invest Opthalmol Vis Sci 32:3131
Nussenblatt RB, Gery I, Ballintine EJ, Wacker WB (1980) Cellular immune responsiveness of unveitis patients to retinal S-antigen. Am J Ophthalmol 89:173
Panek WC, Holland GN, Lee DA, Christensen RE (1990) Glaucoma in patients with uveitis. Br J Ophthalmol 74:223
Park CH, Latina MA (1993) Effects of gamma-interferon on human trabecular meshwork cell phagocytosis. Invest Ophthalmol Vis Sci 34:2228
Podos SM, Becker B, Kass MA (1973) Prostaglandin synthesis, inhibition, and intraocular pressure. Invest Ophthalmol 12:426
Ritch (1981) Pathophysiology of glaucoma in uveitis. Trans Ophthalmol Soc UK 101:321
Roth M, Simmons RJ (1979) Glaucoma associated with precipitates on the trabecular meshwork. Ophthalmology 86:1613
Schlaegel TF Jr (1977) Complications of uveitis. Int Ophthalmol Clin 17:65
Sherwood M, Richardson TM (1980) Evidence for in vivo phagocytosis by trabecular endothelial cells. Invest Ophthalmol Vis Sci [Suppl]:66
Starr MS (1971) Further studies on the effect of prostaglandin on intraocular pressure in the rabbit. Exp Eye Res 11:170
Toris CB, Pederson JE (1987) Aqueous humor dynamics in experimental iridocyclitis. Invest Ophthalmol Vis Sci 28:477
Villumsen J, Alm A (1992) PhXA34-a prostaglandin F2 analogue. Effect on intraocular pressure in patients with ocular hypertension. Br J Ophthalmol 76:214
Wacker WB (1991) Proctor lecture. Experimental allergic uveitis. Investigations of retinal autoimmunity and the immunopathologic responses evoked. Invest Ophthalmol Vis Sci 32:3119
Wacker WB, Donoso LA, Kalsow CM, Yankeelov JA Jr, Organisciak DT (1977) Experimental allergic uveitis: isolation, characterization, and localization of a soluble uveitopathogenic antigen from bovine retina. J Immunol 119:1949
Wakefield D, Lloyd A (1992) The role of cytokines in the pathogenesis of inflammatory eye disease. Cytokine 4:1
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Mermoud, A., Baerveldt, G., Mickler, D.S. et al. Animal model for uveitic glaucoma. Graefe's Arch Clin Exp Ophthalmol 232, 553–560 (1994). https://doi.org/10.1007/BF00181999
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DOI: https://doi.org/10.1007/BF00181999