Abstract
Background: In order to test the influence of a pneumoperitoneum on the peritoneal implantation of free intraperitoneal colon cancer cells, 40 male syngeneic WAG rats were at random divided into four groups.
Methods: Group 1 (n=10) animals underwent a midline laparotomy and 104 CC531 colon cancer cells were injected intraperitoneally (IP); in group 2 (n=10) 104 CC531 cells were injected IP without further manipulation; in group 3 (n=10) a pneumoperitoneum up to 10 mmHg was created after the IP injection of the same amount of CC531 cells. The pneumoperitoneum was maintained for 15 min. Finally in group 4 (n=10) after the IP injection of 104 CC531 cells and after the creation of a pneumoperitoneum, two 14-G IV catheters simulating trocars were introduced in each flank. A follow-up period of 8 weeks was used. Tumor implantation was scored according to the peritoneal cancer index of Eggermont and the index of Chauffert.
Results: Tumor nodules were found varying from 60% in groups 1–3 to 50% in group 4. There was no statistical difference between the implantation rate in the four groups. A port-site recurrence was seen in group 4; all the other tumor implants were located in the mesenterium, omentum, internal genitals, or parietal peritoneum.
Conclusions: The presence of a pneumoperitoneum does not enhance the implantation of free intraperitoneal malignant colon cancer cells in the rat, but the presence of a “port” may lead to abdominal-wall metastases.
Similar content being viewed by others
References
Baer H, Metzger A, Glattli A, Klaiber C, Ruchti C, Czerniak A (1995) Subcutaneous periumbilical metastasis of a gallbladder carcinoma after laparoscopic cholecystectomy. Surg Laparosc Endosc 5: 59–63
Chauffert B, Dimanche-Boitrel MTH, Genne PH, Petit J, Onier N, Jeannin J (1992) Chimiothérapie expérimentale d'une carcinomatose péritonéale d'origine colique chez le rat. Gastroenterol Clin Biol 16: 215–219
Copher J, Rogers J, Dalton M (1995) Trocar-site metastasis following laparoscopic cholecystectomy for unsuspected carcinoma of the gallbladder. Surg Endosc 9: 348–350
Eggermont A, Steller E, Marquet R, Jeekel J, Sugarbaker P (1988) Local regional promotion of tumor growth after abdominal surgery is dominant over immunotherapy with interleukin-2 and lymphokine activated killer cells. Cancer Detect Prev 12: 421–429
Fingerhut A (1995) Laparoscopic colectomy. In: Jager R, Wexner S (eds) The French experience (laparoscopic colorectal surgery). Churchill Livingstone, New York
Hubens G, Lafullarde TH, Vermeulen P, Van Marck E, Hubens A, Eyskens E (1994) Implantation of colon cancer cells on intact and damaged colon mucosa and serosa: an experimental study in the rat. Acta Chir Belg 94: 258–262
Hubens G, Totté E, Verhulst A, Van Marck E, Hubens A (1992) Effect of nonabsorbable and rapidly absorbable suture material on the cytokinetics of crypt cells in colonic anastomosis in the rat. Eur Surg Res 24: 97–102
Hsiu J, Given F, Kemp G (1986) Tumor implantation after diagnostic laparoscopic biopsy of serous ovarian tumours of low malignant potential. Obstet Gynecol 68: 90–93
Jacobi C, Keller H, Monig S, Said S (1995) Implantation metastases of unsuspected gallbladder carcinoma after laparoscopy. Surg Endosc 9: 351–352
Murthy S, Summaria L, Miller R, Wyse T, Goldschmidt R, Scanion E (1991) Inhibition of tumor implantation at sites of trauma by plasminogen activators. Cancer 68: 1724–1730
Nduka C, Monson J, Menzies-Gow N, Darzi A (1994) Abdominal wall metastases following laparoscopy. Br J Surg 81: 648–652
Prasad A, Avery C, Foley R (1994) Abdominal wall metastases following laparoscopy. Br J Surg 81: 1697 (letter)
Stockdale A, Pocock T (1985) Abdominal wall metastases following laparoscopy: a case report. Eur J Surg Oncol 11: 373–375
Wexner S, Cohen S (1995) Port site metastases after laparoscopic colorectal surgery for cure of malignancy. Br J Surg 82: 295–298
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hubens, G., Pauwels, M., Hubens, A. et al. The influence of a pneumoperitoneum on the peritoneal implantation of free intraperitoneal colon cancer cells. Surg Endosc 10, 809–812 (1996). https://doi.org/10.1007/BF00189539
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00189539