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Identification and morphometric evaluation of the synapses of optic nerve afferents in the optic tectum of the axolotl (Ambystoma mexicanum)

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Summary

The terminals of retinal afferents in the tectum of the axolotl have been identified ultrastructurally using techniques of horseradish peroxidase-filling and degeneration. The mitochondria in filled structures show a characteristic electron-lucent matrix. After both eyes have been removed, terminals with light mitochondria disappear from the area known to receive an optic input. In this area the presence of light mitochondria is almost always diagnostic of the retinal origin of a bouton. The synapses are similar to those assumed to be of retinal origin in other vertebrates. Detailed morphometric analysis has been carried out on identified optic synapses in the optic tectum of the axolotl.

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References

  • AkertK, Pfenniger K, Sandri C, Moor H (1972) Freeze etching and cytochemistry of vesicles and membrane complexes in synapses of the central nervous system. In: GD Pappas and DP Purpura (eds) Structure and function of synapses. Raven Press, New York, pp 67–86

    Google Scholar 

  • Angaut P, Repérant J (1976) Fine structure of the optic fibre termination layers in pigeon optic tectum: A Golgi and E.M. study. Neurosci 1:93–105

    Google Scholar 

  • Colonnier M (1968) Synaptic patterns on different cell types in the different laminae of the cat visual cortex. An electron microscopic study. Brain Res 9:268–287

    Google Scholar 

  • Cullen MJ, Kaiserman-Abramof IR (1976) Cytological organization of the dorsal lateral geniculate nuclei in mutant, anophthalmic, and postnatally enucleated mice. J Neurocytol 5:407–424

    Google Scholar 

  • Gray EG (1959) Axo-somatic and axo-dendritic synapses of the cerebral cortex: an electron microscope study. J Anat (Lond) 93:420–432

    Google Scholar 

  • Gruberg ER (1969) Functional organization of the tectum of the tiger salamander Ambystoma tigrinum. PhD Thesis, University of Illinois Urbana Ill

  • Güldner F-H (1978a) Synapses of optic nerve afferents in the rat suprachiasmatic nucleus. I. Identification, qualitative description, development and distribution. Cell Tissue Res 194:17–35

    Google Scholar 

  • Güldner F-H (1978b) Synapses of optic nerve afferents in the rat suprachiasmatic nucleus. II. Structural variability as revealed by morphometric examination. Cell Tissue Res 194:37–54

    Google Scholar 

  • Guillery RW, Colonnier M (1970) Synaptic patterns in the dorsal lateral geniculate nucleus of the monkey. Z Zellforsch 103:90–108

    Google Scholar 

  • Guillery RW, Updyke BV (1976) Retinofugal pathways in normal and albino axolotls. Brain Res 109:235–244

    Google Scholar 

  • Hayes BP, Webster KE (1975) An electron microscope study of the retino-receptive layers of the pigeon optic tectum. J Comp Neurol 162:447–466

    Google Scholar 

  • Herrick CJ (1942) Optic and postoptic systems in the brain of Ambystoma tigrinum. J Comp Neurol 77:191–353

    Google Scholar 

  • Herrick CJ (1948) The brain of the tiger salamander Ambystoma tigrinum. University of Chicago Press

  • Ingham CA, Güldner F-H (1980) Constant occurrence of an ipsilateral retino-tectal projection in the axolotl (Ambystoma mexicanum) revealed by horseradish peroxidase tracing. Neurosci Lett 17:17–22

    Google Scholar 

  • Laufer M, Vanegas H (1974) The optic tectum of a perciform teleost II. Fine structure. J Comp Neurol 154:61–96

    Google Scholar 

  • Le Vay S (1971) On neurons and synapses of the lateral geniculate nucleus of the monkey and the effects of eye enucleation. Z Zellforsch 113:396–419

    Google Scholar 

  • Lewis PR, Knight DP (1977) Staining methods for sectioned material. In: AM Glauert (ed) Practical methods in electron microscopy, V 5, Pt 1. North-Holland, Amsterdam New York Oxford, pp 45–46

    Google Scholar 

  • Lieberman AR, Webster KE (1974) Aspects of the synaptic organization of intrinsic neurons in the dorsal lateral geniculate nucleus. J Neurocytol 3:677–710

    Google Scholar 

  • Lund RD (1969) Synaptic patterns of the superficial layers of the superior colliculus of the rat. J Comp Neurol 135:179–208

    Google Scholar 

  • Lund RD (1972) Synaptic patterns in the superficial layers of the superior colliculus of the monkey Macaca mulatta. Exp Brain Res 15:194–211

    Google Scholar 

  • Marotte LR, Mark RF (1975) Ultrastructural localization of synaptic input to the optic lobe of Carp (Carassius carassius). Exp Neurol 49:722–789

    Google Scholar 

  • Mathers LHJnr (1977) Retinal and visual cortical projection to the superior colliculus of the rabbit. Exp Neurol 57:698–712

    Google Scholar 

  • Palay SL, Chan-Palay V (1975) A guide to the synaptic analysis of the neuropile. In: The Synapse. Cold Spring Harb Quant Biol 40:1–16

    Google Scholar 

  • Potter HD (1969) Structural characteristics of cell and fiber populations in the optic tectum of the frog (Rana catesbeiana). J Comp Neurol 136:203–232

    Google Scholar 

  • Reynolds ES (1963) The use of lead citrate of high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17:208–213

    Article  CAS  PubMed  Google Scholar 

  • Robson JA, Mason CA (1979) The synaptic organization of terminals traced from individual labelled retino-geniculate axons in the cat. Neurosci 4:99–111

    Google Scholar 

  • Sterling P (1971) Receptive fields and synaptic organization of the superficial gray layer of the cat superior colliculus. Vision Res 11: (Suppl 3) 309–328

    Google Scholar 

  • Székely G, Lázár G (1976) Cellular and synaptic architecture of the optic tectum. In: R Llinás and W Precht (eds) Frog Neurobiology. Springer Verlag, New York Heidelberg Berlin, pp 407–434

    Google Scholar 

  • Székely G, Sétáló G, Lázár G (1973) Fine structure of frogs optic tectum: optic fibre termination layers. J Hirnforsch 14:189–225

    Google Scholar 

  • Szentágothai J (1973) Neuronal and synaptic architecture of the lateral geniculate nucleus. In: R Jung (ed) Handbook of sensory physiology, Volume VII/3B. Springer Verlag, Berlin Heidelberg New York, pp 141–176

    Google Scholar 

  • Szentágothai J, Hámori J, Tömböl T (1966) Degeneration and electron microscope analysis of the synaptic glomeruli in the lateral geniculate body. Exp Brain Res 2:283–301

    Google Scholar 

  • Williams MA (1977) Quantitative methods in biology. In: AM Glauert (ed) Practical methods in electron microscopy, 6, II. North Holland, Amsterdam, p 52

  • Yamada H (1974) Light and electron microscope analysis of the medial terminal nucleus of the accessory optic system in the mouse. J Mikrosk Anat Forsch 88:997–1017

    Google Scholar 

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Authors and Affiliations

  1. Department of Behavioural Biology, Research School of Biological Sciences, The Australian National University, 2601, Canberra, A.C.T., Australia

    C. A. Ingham & F. -H. Güldner

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  1. C. A. Ingham
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  2. F. -H. Güldner
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Ingham, C.A., Güldner, F.H. Identification and morphometric evaluation of the synapses of optic nerve afferents in the optic tectum of the axolotl (Ambystoma mexicanum). Cell Tissue Res. 214, 593–611 (1981). https://doi.org/10.1007/BF00233499

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