Summary
This pilot study was designed to test the feasibility of using purified clostridial collagenase in the clinical management of Peyronie's disease. The basic properties of this agent are discussed. We studied its effect on Peyronie's plaque tissue by a quantitative in vitro assay utilising the liberation of free α-amino groups as an index of enzymatic collagenolysis. Tissue from three patients with Peyronie's disease was used. Tunica albuginea from a second group of three normal patients was studied in the same manner, and no selectivity for the collagen of Peyronie's plaques was identified. Utilising human pericardium as a uniform collagenous substrate, a simple dose-effect relationship was established, and the distribution characteristics of injected collagenase observed. Its effects on blood vessels and nerves in vivo was determined as well as the effects of collagenase on the histology of normal and diseased human tissue in vitro. A tentative dose for use in Peyronie's disease was established, which is discussed in light of existing toxicological data. The study was designed to test the eeasibility of purified collagenase in the clinical management of Peyronie's disease. Data included detail plaque digestion and dose-effect relationships in vitro, as well as the histological effects on plaques, blood vessels, and nerves in vivo and in vitro. It is concluded that collagenase may warrant further clinical testing in the treatment of Peyronie's disease.
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References
Oakley CL, Warrack GH (1950) The Alpha, Beta and Gamma Antigens of Clostridium Histolyticum (Weinberg and Seguin, 1916). J Gen Microbiol 4:365
Mandl I, MacLennan JD, Howes EL, DeBellis RH, Sohler A (1953) Isolation and Characterization of Proteinase and Collagenase from Cl. Histolyticum. J Clin Invest 32:1323
Mandl I, Zipper H, Ferguson LT (1958) Clostridium Histolyticum Collagenase: Its Purification and Properties. Arch Biochem Biophys 74:465
Mandel I, Keller S, Manahan J (1964) Multiplicity of Clostridium Histolyticum Collagenase. Biochem 3:1737
Schaud MC, Stauch L (1968) Purification of Collagenase. IV. Hoppe Seyler's Z Physiol Chem 349:809
Yoshida E, Noda H (1965) Isolation and Characterization of Collagenase I and II from Clostridium Histolyticum. Biochem Biophys Acta 105:562
Gross J, Lapiere CM (1962) Collagenolytic Activity in Amphibian Tissues: A Tissue Culture Assay. Proc Natl Acad Sci USA 48:1014
Harris ED Jr., Krane SM (1974) Collagenases (3 parts). NEJM 291(12):605, 291(13):652
Perez-Tamayo R (1978) Pathology of Collagen Degradation. Am J Path 92(2):509
Gross J (1973) Collagen Biology: Structure, Degradation and Disease. J Harvey Lect 68:351
Sussman BJ, Mann M (1969) Experimental Intervertebral Discolysis with Collagenase. J Neurosurg 31:628
Garvin PJ (1974) Toxicity of Collagenase: The Relation to Enzyme Therapy of Disc Herniation. Clin Orthop Rel Res 101:286
Varma AO, Bugatch E, German FM (1973) Debridement of Dermal Ulcers with Collagenase. SG&O 136:781
Sussman J, Bromly JW, Gomez IC (1981) Injection of Collagenase in the treatment of Herniated Lubmar Disc. JAMA 245 (7):730
Moore S, Stein WH (1948) Photometric Ninhydrin Method for Use in the Chromotography of Amino Acids. J Biol Chem 176:367
Lillie BO, Fullmer HM (1976) Histophatologic Technique and Practical Histochemistry. McGraw Hill, New York
Diegelmann RF, Rothkopf LC, Cohen IK (1975) Measurement of Collagen Biosynthes During Wound Healing. J Surg Res 19:239
Stern WE, Coulsen WF (1976) Effects of Collagenase Upon the Intervertebral Disk in Monkeys. J Neurosurg 44:32
Anderson JC (1976) Glycoproteins of the Connective Tissue Matrix. Int Rev Conn Tiss Res 7:251
Mukherjee A, Kahn KP, Ganguly NC, Dey TK (1978) Plastic Induration of Penis: Peyronies Disease — A Histophatological Study. Indian J Path/Micro 21:197
Mandl I (ed) (1972) Collagenase. Gordon and Breach New York
Garvin PJ, Jennings RB, Smith L, Gesler RM (1965) Chymopapain: A Pharmacologic and Toxicologic Evaluation in Experimental Animals. Clincial Orthopedics & Related Research 41:204
Braun WPH (1975) Contact Allergy to Collagenase Mixture (Iruxol). Cont Derm 1:241
Mandl I (1961) Collagenases and Elastases. In: Nord FF (ed) Advances in Enzymology 23:163
Handbook of Experimental Immunology (1976) Weir DM (ed) Blackwell, Oxford
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Gelbard, M.K., Walsh, R. & Kaufman, J.J. Collagenase for Peyronie's disease experimental studies. Urol. Res. 10, 135–140 (1982). https://doi.org/10.1007/BF00255956
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DOI: https://doi.org/10.1007/BF00255956