Summary
In the supernatant of melanoma cell culture SK mel 25 three indolic compounds-5-hydroxy-6-methoxyindole, 5-hydroxy-6-methoxyindolyl-2-carboxylic, and 6-hydroxy-5-methoxyindolyl-2-carboxylic acids-have been identified. The supernatants were extracted with ethyl acetate, derivatized, and analyzed by gas chromatography-mass spectrometry. The significance of this finding is discussed.
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Abbreviations
- 5,6DHI:
-
5,6-dihydroxyindole
- 5H6MI:
-
5-hydroxy-6-methoxyindole
- 6H5MI:
-
6-hydroxy-5-methoxyindole
- 5,6DHI2C:
-
5,6-dihydroxyindolyl-2-carboxylic acid
- 5H6MI2C:
-
5-hydroxy-6-methoxyindolyl-2-carboxylic acid
- 6H5MI2C:
-
6-hydroxy-5-methoxyindolyl-2-carboxylic acid
- PFPA:
-
pentafluoropropionic anhydride
- HFIP:
-
1,1,1,3,3,3-hexafluoroisopropanol
- DOPA:
-
L-3,4-dihydroxyphenylalanine
- COMT:
-
catechol-O-methyltransferase (EC 2.1.1.6)
- GC-MS:
-
gas chromatography-mass spectrometry
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This work was supported by grants from Koningin Wilhelmina Fonds (Netherlands Cancer Foundation) Nos.82-10 and 82-6 GUKC
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Pavel, S., Muskiet, F.A.J., de Ley, L. et al. Identification of three indolic compounds in a pigmented-melanoma cell-culture supernatant by gas chromatography-mass spectrometry. J Cancer Res Clin Oncol 105, 275–279 (1983). https://doi.org/10.1007/BF00395758
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DOI: https://doi.org/10.1007/BF00395758