Abstract
Genetic and structural analyses of the mouse genes encoding constant region of immunoglobulin subclasses (Igh-C) have shown that recombination is rare within this cluster which is inherited as a set designated the Igh haplotype. Recent molecular analyses have demonstrated that either DNA exchanges or gene duplications have probably occurred during the evolution of this set of genes. In order to assess the generality of the duplication processes, the presence and expression of two allelic forms of the Igh-1 (γ2a) gene (Igh-1 a and Igh-1 b) were examined in a large panel of wild mice belonging to Mus musculus domesticus and Mus musculus musculus species. Our data indicate that certain M. m. domesticus animals and most animals in the M. m. musculus group coexpress the two allelic forms of Igh-1. Moreover, genetic studies show that these two immunoglobulin types are encoded by tandemly arranged genes. We propose that wild mice, from which laboratory mice are derived, carry three isotypic γ2 genes (Igh-1 a, Igh-1b, Igh-3), and these have given rise to the two isotypes seen in laboratory strains by a deletion/insertion mechanism.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bonhomme, F.: Evolutionary relationships in the genus Mus. Curr Top Microbial Immunol 127: 19–34, 1986
Bonhomme, F., Catalan, J., Britton-Davidian, J., Chapman, V. M., Moriwaki, K., Nevo, E., and Thaler, L.: Biochemical diversity and evolution in the genus Mus. Biochem Genet 22: 275–303, 1984
Bonhomme, F., Guenet, J. L., Dod, B., Moriwaki, K., and Bulfied, G.: The polyphyletic origin of laboratory inbred mice and their rate of evolution. Biol J Linn Soc 30: 51–58, 1987
Boursot, P., Bonhomme, F., Britton-Davidian, J., Catalan, J., Yonekawa, H., Orsini, P., Gerasimov, S., and Thaler, L.: Introgression differentielle des genomes nucleaires et mitochondriaux chez deux semi-espèces de souris. C R Acad Sci Paris 299: 365–370, 1984
Cazenave, P.-A., Bonhomme, F., Guenet, J. L., and Kindt, T. J.: Correlation of CTβ phenotype with origins of laboratory mouse strains. Curr Top Microbial Immunol 127: 300–306, 1986
Feinberg, A. P. and Vogelstein, B.: A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6–13, 1983
Green, M. C.: Genetic nomenclature for the immunoglobulin loci of the mouse. Immunogenetics 8: 89–99, 1979
Herzenberg, L. A. and Herzenberg, L. A.: Mouse immunoglobulin allotypes: description and special methodology. In D. M. Weir (ed.): Handbook of Experimental Immunology, Volume 1, Blackwell Scientific Publications, Oxford, pp. 12–23, 1978
Huang, C. M., Parsons, M., Wakeland, E. K., Moriwaki, K. E., and Herzenberg, L. A.: New immunoglobulin IgG allotypes and haplotypes found in wild mice with monoclonal anti-allotope antibodies. J Immunol 128: 661–667, 1982
Huang, C. M., Parsons, M., Oi, V. T., Huang, H.-J. S., and Herzenberg, L. A.: Genetic characterization of mouse immunoglobulin allotypic determinants (allotopes) defined by monoclonal antibodies. Immunogenetics 18: 311–321, 1983
Huppi, K., Jouvin-Marche, E., Scott, C., Potter, M., and Weigert, M.: Genetic polymorphism at the κ chain loci in mice: comparisons of restriction enzyme hybridization fragments of variable and constant region genes. Immunogenetics 21: 445–457, 1985
Lieberman, R.: Genetics of IgCH (allotype) locus in the mouse. Springer Semin Immunopathol 1: 7–30, 1978
Lieberman, R. and Potter, M.: Crossing over between genes in the immunoglobulin heavy chain linkage group of the mouse. J Exp Med 130: 519–541, 1969
Oi, V. T., Herzenberg, L. A., and Birstein, B. K.: Localisation of murine Igh-Ia allotypic determinants by using a panel of mouse myeloma variant immunoglobulins. J Immunol 130: 1967–1969, 1983
Ollo, R. and Rougeon, F.: Gene conversion and polymorphism: generation of mouse immunoglobulin γ2a chain alleles by differential gene conversion by 2b chain gene. Cell 32: 515–523, 1983
Ollo, R., Auffray, C., Morchamps, C., and Rougeon, F.: Comparison of mouse immunoglobulin gamma-2a and gamma-2b chain genes suggest that exons can be exchanged between genes in a multigenic family. Proc Natl Acad Sci USA 78: 2442–2446, 1981
Parsons, M., Cazenave, P.-A., and Herzenberg, L. A.: Igh-4d, a new allotype at the mouse IgG1 heavy chain locus. Immunogenetics 14: 341–344, 1981
Schreier, P., Bothwell, A. L. M., Mueller-Hill, B., and Baltimore, D.: Multiple differences between the nucleic acid sequences of the IgG2a alleles of the mouse. Proc Natl Acad Sci USA 78: 4495–4499, 1981
Shimizu, A., Takahahi, N., Yamawaki-Kataoka, Y., Nishida, Y., Katakoaka, T., and Honjo, T.: Ordering of mouse immunoglobulin heavy chain genes by molecular cloning. Nature 289: 149–153, 1981
Shimizu, A., Takahashi, N., Yaoita, Y., and Honjo, T.: Organization of the constant-region gene family of the mouse immunoglobulin heavy chain. Cell 28: 499–506, 1982a
Shimizu, A., Hamaguchi, Y., Yaoita, Y., Moriwaki, K., Kondo, K., and Honjo, T.: Japanese wild mouse. Mus Musculus molossinus, has duplicated immunoglobulin γ2a genes. Nature 298: 82–84, 1982b
Sonoda, S. and Schlanowitz, M.: Studies of 125I trace labeling of immunoglobulin G by chloramine-T. Immunochemistry 7: 885, 1970
Southern, E. M.: Detection of specific sequence among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–517, 1975
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jouvin-Marche, E., Morgado, M.G., Leguern, C. et al. The mouse Igh-1 a and Igh-1 b H chain constant regions are derived from two distinct isotypic genes. Immunogenetics 29, 92–97 (1989). https://doi.org/10.1007/BF00395856
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00395856