Summary
In this study we report the localisation of PYY immunoreactivity in intestinal mucosa endocrine (EG) cells containing glucagon-related peptides and also in foetal pancreatic A cells of rat and man. Radioimmunoassay of human foetal pancreatic extracts revealed the presence of PYY immunoreactivity, the concentration of which declined with age (from 65.42 pmol/g at week 20 to 17.0 pmol at week 40; correlation coefficient=−0.893), in contrast to the amount of glucagon which remained statistically constant throughout the same foctal period. The identity of this PYY immunoreactive material with the original 36 amino acid porcine peptide has been shown by high pressure liquid chromatography (HPLC).
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Adrian TE, Bloom SR, Bryant MG, Polak JM, Heitz Ph, Barnes AJ (1976) Distribution and release of human pancreatic polypeptide. Gut 17:940–944
Adrian TE, Bacarese-Hamilton AJ, Allen JM, Tatemoto K, Ferri G-L, Polak JM, Bloom SR (1982) Distribution of PYY in the porcine and human gastrointestinal tract. Regul Pept 4:355
Bateille D, Tatemoto K, Gespach C, Jörnvall H, Rosselin G, Mutt V (1982) Isolation of glucagon-37 (bioactive enteroglucagon/oxyntomodulin) from porcine jejuno-ileum: Characterization of the peptide. FEBS Lett 146:79–86
Bell GJ, Santerret RF, Mullenbach GT (1983) Hamster preproglucagon contains the sequence of glucagon and two related peptides. Nature 302:716–718
El Salhy M, Wilander E, Grimelius L, Terenius L, Lundberg JM, Tatemoto K (1982a) The distribution of polypeptide YY (PYY) and pancreatic polypeptide (PP) immunoreactive cells in the domestic fowl. Histochemistry 75:25–30
El Salhy M, Grimelius L, Lundberg JM, Tatemoto K, Terenius L (1982b) Immunocytochemical evidence for the occurrence of PYY, a newly isolated gut polypeptide in endocrine cells in the gut of amphibians and reptiles. Biomed Res 3:303–306
El Salhy M, Wilander E, Abu Sinna G (1982c) The endocrine pancreas of anuran amphibians: A histological and immunocytochemical study. Biomed Res 3:579–589
Falkmer S, Van Noorden S (1983) Ontogeny and phylogeny of the glucagon cell. In: Lefebvre PJ (ed) Handbook of experimental pharmacology. Springer, Berlin Heidelberg New York, pp 81–119
Huang W-M, Gibson SJ, Facer P, Gu J, Polak JM (1983) Improved section adhesion for immunocytochemistry using high molecular weight polymers of l-lysine as a slide coating. Histochemistry 77:275–279
Kaung HL, Elde RP (1980) Distribution and morphometric quantitation of pancreatic endocrine cell types in the frog Rana pipiens. Anat Rec 196:173–181
Leduque P, Paulin C, Dubois PM (1983) Immunocytochemical evidence for a substance related to the bovine pancreatic polypeptide-peptide YY group of peptides in the human foetal gastrointestinal tract. Regul Pept 6:219–230
Lehy T, Peranzi G, Christina ML (1981) Correlating immunocytochemical and electron microscopic studies: Identification of entero(glucagon)-, somatostatin and pancreatic polypeptide-like containing cells in the human colon. Histochemistry 71:67–80
Lopez LC, Frazier ML, Su C-J, Kumar A, Saunders GF (1983) Mammalian pancreatic preproglucagon contains three glucagon-related peptides. Proc Natl Acad Sci USA 80:5485–5489
Lund PK, Goodman RH, Dee PC, Habener JF (1982) Pancreatic preproglucagon cDNA contains two glucagon-related coding sequences arranged in tandem. Proc Natl Acad Sci USA 79:345–349
Lundberg JM, Tatemoto K, Terenius L, Hellstrom PM, Mutt V, Hökfelt T, Hamberger B (1982) Localisation of peptide YY (PYY) in gastrointestinal endocrine cells and effects on intestinal blood flow and motility. Proc Natl Acad Sci USA 79:4471–4475
Rall LB, Pictet RL, Williams RH, Rutter WJ (1973) Early differentiation of glucagon-producing cells in embryonic pancreas: A possible developmental role for glucagon. Proc Natl Acad Sci USA 70:3478–3482
Ravazzola M, Orci L (1980a) Transformation of glicentin-containing L-cells into glucagon-containing cells by enzymatic digestion. Diabetes 29:156–158
Ravazzola M, Orci L (1980b) A pancreatic polypeptide (PP) like immunoreactant is present in the glicentin-containing cell of the cat intestine. Histochemistry 67:221–224
Ravazzola M, Siperstein A, Moody AJ, Sundby F, Jacobsen, H, Orci L (1979) Glicentin immunoreactive cells: Their relationship to glucagon-producing cells. Endocrinology 105:499–508
Solcia E, Buffa R, Capella C, Fiocca R, Fontana P, Civelli O (1979) Immunohistochemical characterisation of gastroenteropancreatic endocrine cells and related multihormonal cells. Problems, pitfalls and facts. In: Miyoshi Y (ed) Gut peptides: Secretion, function and clinical aspects. Elsevier Biomedical Press, Amsterdam, pp 303–309
Sundby F, Jacobsen H, Moody AJ (1976) Purification and characterisation of a protein from porcine gut with glucagon-like immunoreactivity. Horm Metab Res 8:366–371
Sternberger LA (1979) Immunocytochemistry. 2nd edn, J. Wiley, New York
Tager HS, Steiner DF (1973) Isolation of a glucagon-containing peptide: Primary structure of a possible fragment of proglucagon. Proc Natl Acad Sci USA 70:2321–2325
Tatemoto K (1982) Isolation and characterisation of peptide YY (PYY), a candidate gut hormone that inhibits pancreatic exocrine secretion. Proc Natl Acad Sci USA 79:2514–2518
Tatemoto K, Carlquist M, Mutt V (1982) Neuropeptide Y: A novel brain peptide with structural similarities to peptide YY and pancreatic polypeptide. Nature 296:659–660
Thim L, Moody AJ (1980) The primary structure of porcine glicentin (proglucagon). Regul Pept 2:139–150
Vaillant C, Taylor IL (1981) Demonstration of carboxyl-terminal PP-like peptides in endocrine cells and nerves. Peptides 2:31–35
Varndell IM, Tapia FJ, Probert L, Buchan AMJ, Gu J, De Mey J, Bloom SR, Polak JM (1982) Immunogold staining procedure for the localisation of regulatory peptides. Peptides 3:259–272
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Ali-Rachedi, A., Varndell, I.M., Adrian, T.E. et al. Peptide YY (PYY) immunoreactivity is co-stored with glucagon-related immunoreactants in endocrine cells of the gut and pancreas. Histochemistry 80, 487–491 (1984). https://doi.org/10.1007/BF00495439
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DOI: https://doi.org/10.1007/BF00495439