Summary
Uraemia was induced in pigs by ligation of the renal vascular pedicle, and uraemic plasma was analysed for glucagon and glucagon-related peptides. A preponderance of large molecular weight (Mr) components comprising glicentin and moieties of slightly lower Mr was found, accounting for 73 ± 3% (mean ± SEM, n =12) of the total plasma glucagon-like immunoreactivity. Comparisons with glicentin 1–61, produced by controlled, stepwise, consecutive digestion of purified natural glicentin with carboxypeptidases (carboxypeptidase A followed by carboxypeptidase B, and again by carboxypeptidase A and B), gel filtration, ion exchange chromatography, reverse phase HPLC and radioimmunoassays for the glucagon sequences 6–15 and 19–29 and for the glicentin sequence 12–30 all indicate that glicentin 1–61 constitutes approximately 57% of the large Mr glucagon-related peptides found in uraemia in pigs.
Article PDF
Similar content being viewed by others
References
Lefebvre PJ, Luyckx AS, Nizet AH (1974) Renal handling of endogenous glucagon in the dog. Metabolism 23: 753–761
Lefebvre PJ, Luyckx AS (1975) Effects of acute kidney exclusion by ligation of renal arteries on peripheral plasma glucagon levels and pancreatic glucagon production in the anesthetized dog. Metabolism 24:1169–1176
Bilbrey GL, Faloona GR, White MG, Atkins C, Hull AR, Knochel JP (1975) Hyperglucagonemia in uremia: reversal by renal transplantation. Ann Intern Med 82: 525–528
Kuku SF, Jaspan JB, Emmanouel DS, Zeidler A, Katz AI, Rubinstein AH (1976) Heterogeneity of plasma glucagon circulating components in normal subjects and patients with chronic renal failure. J Clin Invest 58: 742–750
Emmanouel DS, Jaspan JB, Kuku SF, Rubinstein AH, Katz AI, Huen AHJ (1976) Pathogenesis and characterization of hyperglucagonemia in the uremic rat. J Clin Invest 58: 1266–1272
Jaspan JB, Rubinstein AH (1977) Circulating glucagon. Plasma profiles and metabolism in health and disease. Diabetes 26: 887–902
Jaspan JB, Polonski KS, Rubinstein AH (1981) The heterogeneity of immunoreactive glucagon in plasma: clinical implications. In: Unger RH, Orci L (eds) Glucagon. Physiology, pathophysiology and morphology of the pancreatic A cell. Elsevier/North Holland, New York, pp 77–96
Hoist JJ (1983) Molecular heterogeneity of glucagon in normal subjects and in patients with glucagon producing tumors. Diabetologia 24:359–365
Hoist JJ, Hoist Pedersen J, Baldissera F, Stadil F (1983) Circulating glucagon after total pancreatectomy in man. Diabetologia 25: 396–399
Thim L, Moody AJ (1981) The primary structure structure of porcine glicentin (proglucagon). Regul Peptides 2: 139–141
Baldissera FGA, Munoz-Perez MA, Hoist JJ (1983) Somatostatin 1–28 circulates in human plasma. Regul Peptides 6: 63–69
Hoist JJ (1980) Evidence that glicentin contains the entire sequence of glucagon. Biochem J (London) 187: 337–343
Hoist JJ (1982) Evidence that enteroglucagon (II) is identical with C-terminal sequence (residues 33-69) of glicentin. Biochem J (London) 207:381–388
Thim L, Moody AJ (1982) Purification and chemical characterization of a glicentin related pancreatic peptide (proglucagon fragment) from porcine pancreas. Biochem Biophys Acta 703: 134–141
Moody AJ, Hoist JJ, Thim L, Jensen SL (1981) Relationship of glicentin to proglucagon and glucagon in the porcine pancreas. Nature 289: 514–516
Stadil F, Rehfeld JF (1973) Determination of gastrin in serum. An evaluation of the reliability of a radioimmunoassay. Scand J Gastroenterol 8: 101–112
Hoist JJ (1977) Extraction, gel filtration pattern and receptor binding of porcine gastrointestinal glucagon-like immunoreactivity. Diabetologia 13: 159–169
Bell GI, Santerre RF, Mullenbach GT (1983) Hamster pre-proglucagon contains the sequence of glucagon and two related peptides. Nature 302: 716–718
Hoist JJ (1983) Gut glucagon, enteroglucagon, gut glucagon-like immunoreactivity, glicentin-current status. Gastroenterology 84: 1602–1613
Ambler RP (1967) Enzymic hydrolysis with carboxipeptidases. In: Hirs CHW (ed) Methods in enzymology, vol XI. Academic Press, New York, pp 155–166
Yanaihara C, Matsumoto T, Kadowaki M, Iguchi K, Yanaihara N (1985) Rat pancreas contains the proglucagon (64–69) fragment and arginine stimulates its release. FEBS Lett 187: 307–310
Rigopoulou D, Valverde I, Marco J, Faloona G, Unger RH (1970) Large glucagon immunoreactivity in extracts of pancreas. J Biol Chem 245: 496–501
Sheikh SP, Baldissera FGA, Karlsen FØ, Hoist JJ (1985) Glicentin is present in the pig pancreas. FEBS Lett 179: 1–6
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Baldissera, F.G.A., Holst, J.J. Glicentin 1–61 probably represents a major fraction of glucagon-related peptides in plasma of anaesthetized uraemic pigs. Diabetologia 29, 462–467 (1986). https://doi.org/10.1007/BF00506540
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00506540