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Plasma protein handling in the rat kidney: Micropuncture experiments in the acute heterologous phase of Anti-GBM-nephritis

  • Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands
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Abstract

Glomerular filtration and tubular uptake of plasma proteins have been studied in the rat using micropuncture techniques.

  1. 1.

    Under control conditions the glomerular capillary wall is an effective barrier, only 7.6 μg/min×100 g BW albumin have been measured as filtered load. Four to twelve hours after i.v. injection of anti-glomerularbasement membrane serum (anti-GBM-serum) sieving coefficient π and filtered load increased in a dose-dependent manner (π albumin in controls=0.27×10−3, after injection of 0.5 ml Antiserum π=0.28×10−3 and 1.0 ml Antiserum π=2.32 ¢10−3).

  2. 2.

    The tubular reabsorption capacity is almost reached under control conditions and amounts to 5.6–10.7 μg/min×100 g BW for albumin. Only reduced GFR (0.36±0.07 ml/min×100 g BW) and reduced tubular flow lead to increased tubular uptake under overload conditions (10.7 vs. 99.0 μg albumin/min×100 g BW).

  3. 3.

    Tubular reabsorption of so-called high-molecular-weight proteins seems to be a nonselective mechanism.

The ratio Alb/Alb+Glob (89.9–93.1%) did not differ significantly at the individual puncture sites and in the final urine.

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References

  1. Allison, M. E. M., Wilson, C. B., Gottschalk, C. W.: Pathophysiology of experimental glomerulonephritis in rats. J. Clin. Invest.53, 1402–1423 (1974)

    Google Scholar 

  2. Baldamus, C. A., Galaske, R. G., Eisenbach, G. M. Krause, H. P., Stolte, H.: Glomerular protein filtration in normal and nephritic rats: a micropuncture study. Contributions to nephrology: Vol. 1, pp. 37–49, Basel-New York: S. Karger 1975

    Google Scholar 

  3. Bell, E. T.: Renal lesions associated with multiple myeloma. Am. J. Path.9, 393–420 (1933)

    Google Scholar 

  4. Beneke, G.: Amyloid und Niere. In: Fortschritte der Nephrologie. A. Bohle, G. E. Schubert, eds., pp. 193–206. Stuttgart-New York: F. K. Schattauer 1971

    Google Scholar 

  5. Bienenstock, J., Tomasi, T. B.: The nature of γA in normal urine. In: Proteins in normal and pathological urine, pp. 59–62, Basel-New York: Karger 1970

    Google Scholar 

  6. Blantz, R. C., Wilson, C. B.: Acute effects of antiglomerular basement membrane antibody on the process of glomerular filtration in the rat. J. Clin. Invest.58, 899–911 (1976)

    Google Scholar 

  7. Boesken, W., Meinhart, K., Rentsch, J., Hammer, D.: Characterization of basement membrane antigens present in the urine of normal and nephritic rabbits. Int. Arch. Allergy43, 232–252 (1972)

    Google Scholar 

  8. Bourdeau, J. E., Carone, F. A., Canote, C. E.: Serum albumin uptake in isolated perfused renal tubules. Quantitative and electron microscope radioautographic studies in three anatomical segments of the rat nephron. J. Cell Biol.54, 382–398 (1972)

    Google Scholar 

  9. Brenner, B. M., Troy, J. L., Dougharty, T. M., Deen, W. M.: Dynamics of glomerular ultrafiltration in the rat. II. Plasma-flow dependence of GFR. Am. J. Physiol.223, 1184–1190 (1972)

    Google Scholar 

  10. Bright, R.: Cases and observations illustrative of renal disease accompanied with the secretion of albuminous urine. Guy's Hosp. Rep.1, 338 (1836)

    Google Scholar 

  11. Carone, F. A., von Haam, E. V. H.: Micropuncture study of protein excretion in normal and proteinuric rats. Clin. Res.13, 301 (1965)

    Google Scholar 

  12. Carone, F. A., Post, R. S., Banks, D. B.: Micropuncture study of albumin excretion in the normal rat. Am. J. Path.55, A 19 (1968)

    Google Scholar 

  13. Chang, R. L. S., Deen, W. M., Robertson, Ch. R., Brenner, B. M.: Permselectivity of the glomerular capillary wall: III. Restricted transport of polyanions. Kidney Int.8, 212–218 (1975)

    Google Scholar 

  14. Cochrane, C. G.: Mediation of immunologic glomerular injury. Transplant. Proc.1, 949–958 (1969)

    Google Scholar 

  15. Cochrane, C. G., Unanue, E. R., Dixon, F. J.: A role of polymorphonuclear leucocytes and complement in nephrotoxic nephritis. J. Exp. Med.122, 99–116 (1965)

    Google Scholar 

  16. Cortney, M. A., Sawin, L. L., Weiss, D. D.: Renal tubular protein absorption in the rat. J. Clin. Invest.49, 1–4 (1970)

    Google Scholar 

  17. Dirks, J. H., Clapp, J. R., Berliner, R. W.: The protein concentration in the proximal tubule of the dog. J. Clin. Invest.43, 916–921 (1964)

    Google Scholar 

  18. Eisenbach, G. M., van Liew, J. B., Boylan, J. W.: Effects of angiotensin on the filtration of protein in the rat kidney: a micropuncture study. Kidney Int.8, 80–87 (1975)

    Google Scholar 

  19. Farquhar, M. G.: The primary glomerular filtration barrier-basement membrane of epithelial slits. Kidney Int.8, 197–211 (1975)

    Google Scholar 

  20. Feld, L., van Liew, J. B., Galaske, R. G., Boylan, J. W.: Selectivity of renal injury and proteinuria in the spontaneously hypertensive rat. Kidney Int.12, 332–343 (1977)

    Google Scholar 

  21. Gang, N. F., Kalant, N.: Nephrotoxic serum nephritis. I. Chemical, morphologic and functional changes in the glomerular basement membrane during the evolution of nephritis. Lab. Invest.22, 531–540 (1970)

    Google Scholar 

  22. Gang, N. F., Trachtenberg, E., Allerhand, J., Kalant, N., Mautner, W.: Nephrotoxic serum nephritis. III: correlation of proteinuria, excretion of the glomerular basement membrane-like protein and changes in the ultrastructure of the glomerular basement membrane as visualized with lanthanum. Lab. Invest.23, 436–441 (1970a

    Google Scholar 

  23. Gang, N. F., Mautner, W., Kalant, N.: Nephrotoxic serum nephritis II: Chemical, morphological and functional correlates of glomerular basement membrane at the onset of proteinuria. Lab. Invest.23, 150–157 (1970) b)

    Google Scholar 

  24. Hardwicke, J., Cameron J. S., Harrison, J. F., Hulme, B., Soothill, J. F.: Proteinuria, studied by clearances of individual macromolecules. In: Proteins in normal and pathological urine, pp. 111–152. Basel: Karger 1970

    Google Scholar 

  25. Krakower, C. A., Greenspon, S. A.: Localisation of the nephrotoxic antigen within the isolated renal glomerulus Arch. Path.51, 629–639 (1951)

    Google Scholar 

  26. Kühn, K. W., Ryan, G. B., Hein, S. T., Galaske, R. G., Karnovsky, M. J.: An ultrastructural study of mechanism of proteinuria in rat nephrotoxic nephritis. Lab. Invest.36, 375–387 (1977)

    Google Scholar 

  27. Lambert, P. P., Gassée, J. P., Askenasi, R.: Physiological basis of protein excretion. In: Proteins in normal and pathological urine, pp. 67–82. Basel-New York: Karger 1970

    Google Scholar 

  28. Landwehr, D. M., Carvalho, J. S., Oken, D. E.: Micropuncture studies of the filtration and absorption of albumin by nephrotic rats. Kidney Int.11, 9–17 (1977)

    Google Scholar 

  29. Lannigan, R., Kark, R. M., Pollak, V. E.: The effect of a single intravenous injection of amino nucleoside of puromycin on the rat kidney. A light and electron microscopic study. J. Path. Bact.83, 357–362 (1962)

    Google Scholar 

  30. Leber, P. D., Marsh, D. J.: Micropuncture study of concentration and fate of albumin in rat nephron. Am. J. Physiol.219, 358–363 (1970)

    Google Scholar 

  31. Lehmann, D. N., Wilson, C. B., Dixon, F. J.: Interstitial nephritis in rats immunized with heterologous tubular basement membrane. Kidney Int.5, 187–195 (1974)

    Google Scholar 

  32. Lewy, J. E., Pesce, A. J.: Micropuncture study of albumin transfer in aminonucleoside nephrosis in the rat. Pediat. Res.7, 553–559 (1973)

    Google Scholar 

  33. Lowry, O. H., Rosebrough, N. J., Farr, A. C.: Protein measurement with the Folin phenol reagent. J. Biol. Chem.193, 265–275 (1951)

    Google Scholar 

  34. Lubowitz, H., Mazumdar, D. C., Kawamura, J., Crosson, J. T., Weisser, F., Rolf, D., Bricker, N. S.: Experimental glomerulonephritis in the rat: structural and functional observations. Kidney Int.5, 356–364 (1974)

    Google Scholar 

  35. Maack, T. M.: Renal Handling of low molecular weight proteins. Am. J. med.58, 57–64 (1975)

    Google Scholar 

  36. Maack, T. M.: Handling of proteins by the normal kidney. Am. J. Med.56, 71–82 (1974)

    Google Scholar 

  37. Maack, T., Mackensie, D. D. S., Kinter, W. B.: Intracellular pathways of renal reabsorption of lysozyme. Am. J. Physiol.221, 1609–1616 (1971)

    Google Scholar 

  38. Maddox, D. A., Bennett, C. M., Deen, W. M., Glassock, R. J., Knutson, D., Daugharty, T. M., Brenner, D. M.: Determinants of glomerular filtration in experimental glomerular nephritis in the rat. J. Clin. Invest.55, 305–318 (1975)

    Google Scholar 

  39. Masugi, M.: Über die experimentelle Glomerulonephritis durch das spezifische Antinierenserum. Ein Beitrag zur Pathogenese der diffusen Glomerulonephritis. Beitr. Path. Anat.92, 429–466 (1934)

    Google Scholar 

  40. Maunsbach, A. B., Christensen, E. J.: Uptake and digestion of Low Molecular Weight Proteins in the rat kidney tubules. Proceed. VIth Int. Congr. Nephrol., pp. 387–391, Basel-New York: S. Karger 1976

    Google Scholar 

  41. Neuhoff, V.: Micro-disc-Electrophorese von Hirnproteinen. Arzneim.-Forsch.18, 35–39 (1968)

    Google Scholar 

  42. Neuhoff, V.: Micromethods in molecular biology. (V. Neuhoff, ed.), pp. 1–84. Berlin-Heidelberg-New York: Springer 1973

    Google Scholar 

  43. Oken, D. E.: Quantification of picogram quantities of serum albumin by ultramicrodics-electrophoresis and direct densitometry. Microchem. J.15, 557–563 (1970)

    Google Scholar 

  44. Oken, D. E., Flamenbaum, W.: Micropuncture studies of proximal tubule albumin concentrations in normal and nephrotic rats. J. Clin. Invest.50, 1498–1505 (1971)

    Google Scholar 

  45. Oken, D. E., Cotes, S. C., Mende, C. W.: Micropuncture study of tubular transport of albumin in rats with aminonucleoside nephrosis. Kidney Int.1, 3–11 (1972)

    Google Scholar 

  46. Pappenheimer, J. R.: Über die Permeabilität der Glomerulummembranen in der Niere. Klin. Wochenschr.33, 362–365 (1955)

    Google Scholar 

  47. Pollak, V. E., First, M. R., Pesce, A. J.: Value of the sieving coefficient in the interpretation of renal protein clearances. Nephron13, 82–92 (1974)

    Google Scholar 

  48. Raguse, G.: Experimentelle Untersuchungen über die renale Proteinausscheidung beim Schleimaal (Myxine glutinosa). Thesis, Med. School Hannover, Germany (1976)

    Google Scholar 

  49. Rennke, H. G., Venkatachalam, M. A.: Glomerular permeability: in vivo tracer studies with polyanionic and polycationic ferritins. Kidney Int.11, 44–53 (1977)

    Google Scholar 

  50. Rocha, A., Mercondes, M., Malnic, G.: Micropuncture study in rats with experimental glomerulonephritis. Kidney Int.3, 14–23 (1973)

    Google Scholar 

  51. Roy, A. K., Neuhaus, O. W.: Identification of rat urinary proteins by zone and immunoelectrophoresis. Proc. Soc. Exp. Biol. Med.121, 894–899 (1966)

    Google Scholar 

  52. Ryan, J. B., Karnovsky, M. J.: Distribution of endogenous albumin in the rat glomerulus: Role of hemodynamic factors in glomerular barrier function. Kidney Int.9, 36–45 (1976)

    Google Scholar 

  53. Schwartz, R. H., Lewis, R. A., Schenk, E. A.: Tamm-Horsfall Mucoprotein. III. Potassium dichromate-induced renal tubular damage. Lab. Invest.27, 214–217 (1972)

    Google Scholar 

  54. Sellers, A. L., Smith, S., Marmorston, J., Goodman, Ch. C.: Studies of the mechanism of experimental proteinuria. J. Exp. Med.96, 643–652 (1952)

    Google Scholar 

  55. Shigematsu, H.: Glomerular events during the initial phase of rat Masugi nephritis. Virchows Arch., Abt. B Zellpath.5, 187–200 (1970)

    Google Scholar 

  56. Steinhausen, M.: Eine Methode zur Differenzierung proximaler und distaler Tubuli der Nierenrinde von Ratten in vivo und ihre Anwendung zur Bestimmung tubulärer Strömungs-geschwindigkeiten. Pflügers Arch. Ges. Physiol.277, 23–35 (1963)

    Google Scholar 

  57. Stolte, H., Hare, D., Boylan, J. W.d-Glucose and fluid reabsorption in proximal surface tubule of the rat Kidney. Pflügers Arch.334, 193–206 (1972)

    Google Scholar 

  58. Stolte, H., Kühn, K., Galaske, R. G., Alt, J., Baldamus, C. A., Eisenbach, G. M., Sterzel, R. B.: Function and structure following glomerular permeability changes in anti-GBM nephritis and aminonucleoside nephrosis. Contributions to Nephrology, Vol. 2, pp. 104–144. Basel-New York Karger 1976

    Google Scholar 

  59. Thoenes, W., Langer, K. H.: Relationship between cell structures of renal tubules and transport mechanism. In: Renal transport and diuretics (K. Thurau, H. Jahrmärker, eds.), pp. 37–65. Berlin-Heidelberg-New York: Springer 1969

    Google Scholar 

  60. Tomasi, T. B., Tan, E. M., Solomon, A.: Characteristics of an immune system common to certain external excretions. J. Exp. Med.121, 101–124 (1965)

    Google Scholar 

  61. Ullrich, K. J.: Renal tubular mechanism of organic solute transport. Kidney Int.9, 134–148 (1976)

    Google Scholar 

  62. van Liew, J. B., Buentig, W., Stolte, H., Boylan, J. W.: Protein excretion: micropuncture study of rat capsular and proximal tubule fluid. Am. J. Physiol.219, 299–305 (1970)

    Google Scholar 

  63. von Bayer, H., van Liew, J. B., Klassen, J., Boylan, J. W.: Filtration of protein in the anti-glomerular basement membrane nephritic rat: a micropuncture study. Kidney Int.10, 425–437 (1976)

    Google Scholar 

  64. Waldmann, T. A., Stroeber, W., Mogielnicki, R. P.: The renal handling of low molecular weight proteins. II. Disorders of serum protein, catabolism in patients with tubular proteinuria, the nephrotic syndrome or uremia. J. Clin. Invest.51, 2162–2174 (1972)

    Google Scholar 

  65. Zollinger, H. U.: Über hyalin-tropfige Veränderungen der Nierenhauptstücke als Ausdruck von Eiweißspeicherung. Schweiz. Z. Path.13, 146–163 (1950)

    Google Scholar 

  66. Zwiebel, R., Höhmann, B., Frohnert, P., Baumann, K.: Fluorometrisch-enzymatische Mikro- und Ultramikrobestimmung von Inulin und Glucose. Pflügers Arch.307, 127–132 (1969)

    Google Scholar 

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  1. Department Innere Medizin, Medizinische Hochschule Hannover, Karl-Wiechert-Allee 9, d-3000, Hannover, Germany

    Rainer G. Galaske, Conrad A. Baldamus & Hilmar Stolte

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  1. Rainer G. Galaske
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  2. Conrad A. Baldamus
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Galaske, R.G., Baldamus, C.A. & Stolte, H. Plasma protein handling in the rat kidney: Micropuncture experiments in the acute heterologous phase of Anti-GBM-nephritis. Pflugers Arch. 375, 269–277 (1978). https://doi.org/10.1007/BF00582441

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