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Comparative histological, histochemical, immunohistochemical and biochemical studies on oestrogen receptors, lectin receptors, and Barr bodies in human breast cancer

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Summary

The present study performed on a total of 567 cases of human female breast cancer compares the results of the biochemical assay (dextran-coated charcoal assay=DCC) for oestrogen receptor (ER) with those of several morphological methods developed for the detection of the ER or for the prediction of prognosis by use of other systems (FSA= fluorescent ligand binding assay, ER-ICA=monoclonal antibody assay for ER, LRA=lectin receptor assay using peanut agglutinin, and Barr body estimation). Whereas no correlation at all was observed among the results of the DCC and those of the FSA and Barr body estimation, the ER-ICA and the LRA showed an unanimous tendency towards higher values of ER with increasing intensity of the staining product. The results of the ER-ICA may be expressed by an immuno-reactive score (IRS) calculated from the staining intensity (SI) and the percentage of positive cells (PP). The morphological methods are evaluated with special regard to their correlation with the DCC, their theoretical basis, and their practical application. In summary, the ER-ICA appears to be the sole method directly visualizing the ER protein and - in contrast to the DCC - is therefore completely independent of the content of endogenous or exogenous oestrogens in the tumor tissue. The LRA provides valuable additional information concerning tumour differentiation

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  1. Institut für Pathologie der Dr. Horst-Schmidt-Kliniken, Klinikum der Landeshauptstadt Wiesbaden, Ludwig Erhard-Strasse 100, D-6200, Wiesbaden, Federal Republic of Germany

    Wolfgang Remmele & Ute Hildebrand

  2. Institut für Pathologie der Städtischen Krankenanstalten Krefeld, Klinikum der Landeshauptstadt Wiesbaden, Ludwig Erhard-Strasse 100, D-6200, Wiesbaden, Federal Republic of Germany

    Hermann A. Hienz

  3. Pathologisches Institut der Universität - Ludwig Aschoff-Haus - Freiburg i. Br., Klinikum der Landeshauptstadt Wiesbaden, Ludwig Erhard-Strasse 100, D-6200, Wiesbaden, Federal Republic of Germany

    Peter-Josef Klein

  4. Pathologisches Institut der Universität zu Köln, Klinikum der Landeshauptstadt Wiesbaden, Ludwig Erhard-Strasse 100, D-6200, Wiesbaden, Federal Republic of Germany

    Matthias Vierbuchen

  5. BIOSCIENTIA, Institut für Laboruntersuchungen GmbH, Ingelheim am Rhein, Klinikum der Landeshauptstadt Wiesbaden, Ludwig Erhard-Strasse 100, D-6200, Wiesbaden, Federal Republic of Germany

    Lütje J. Behnken & Bernd Heicke

  6. Institut für Medizinische Dokumentation und Statistik der Universität Mainz, Klinikum der Landeshauptstadt Wiesbaden, Ludwig Erhard-Strasse 100, D-6200, Wiesbaden, Federal Republic of Germany

    Eberhard Scheidt

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  1. Wolfgang Remmele
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Dedicated to Professor Karl Lennert, Kiel, on the occasion of his 65th birthday

Supported in part by the German Cancer Fund (Deutsche Krebshilfe)

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Remmele, W., Hildebrand, U., Hienz, H.A. et al. Comparative histological, histochemical, immunohistochemical and biochemical studies on oestrogen receptors, lectin receptors, and Barr bodies in human breast cancer. Vichows Archiv A Pathol Anat 409, 127–147 (1986). https://doi.org/10.1007/BF00708323

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