Summary
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1.
We investigated the function of monoaminergic neurons in the lower brain stem following subarachnoid hemorrhage (SAH) induced in rats, by measuring monoamine metabolites, usingin vivo microdialysis techniques.
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2.
A dialysis probe was implanted in the nucleus tractus solitarius (NTS) and the perfusates were assayed by high-performance liquid chromatography (HPLC) with electrochemical detection (ECD).
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3.
The main monoamine metabolites measured in the NTS extracellular space were 3,4-dihydroxyphenyl acetic acid (DOPAC), homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA).
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Monoamine metabolites in the rat NTS were nonspecifically increased, at least in the acute phase after cisternal injection of blood or saline.
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5.
The disappearance rates of the 5-HIAA decline and the early phase of DOPAC decline after pargyline administration (75 mg/kg, i.p.) were most rapid at 2 days after the induction of SAH, then recovered gradually.
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6.
These results suggest that functions of noradrenergic and serotonergic neurons in the NTS may be disturbed predominantly in the case of induced vasospasm in rats.
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Hirata, K., Kawano, T. & Mori, K. Changes in monoaminergic neuronal function in the lower brain stem following subarachnoid hemorrhage induced in rats. Cell Mol Neurobiol 13, 639–648 (1993). https://doi.org/10.1007/BF00711563
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DOI: https://doi.org/10.1007/BF00711563