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Subclass distribution of human IgG autoantibodies in pemphigus

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Abstract

The distribution of IgG subclasses in the intercellular substance (ICS) reactive autoantibodies in serum of 10 patients with pemphigus was analyzed by semiquantitative indirect immunofluorescence (IF) using the HP series of monoclonal antibodies specific for the four human IgG subclasses. IgG4 ICS specific autoantibody was present in all 10 sera at a titer of 10 to 320, while IgG1 antibodies were found in 9 of 10 sera at a seemingly lower level. IgG3 autoantibodies were detected in the serum of one patient, only after isolation of IgG using ion-exchange chromatography. Autoantibodies of IgG subclass 2 were not detectable in any of the 10 sera tested. One of the ten patients displayed circulating anti-ICS antibodies of only the IgG4 isotype.

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Authors and Affiliations

  1. Cutaneous Immunopathology Unit and Department of Dermatology, University of Texas Medical School at Houston, 77030, Houston, Texas

    Christian C. Jones & Robert E. Jordon

  2. Division of Rheumatology and Clinical Immunogenetics, Department of Internal Medicine, The University of Texas Medical School at Houston, 77030, Houston, Texas

    Robert G. Hamilton

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  1. Christian C. Jones
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  2. Robert G. Hamilton
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  3. Robert E. Jordon
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Jones, C.C., Hamilton, R.G. & Jordon, R.E. Subclass distribution of human IgG autoantibodies in pemphigus. J Clin Immunol 8, 43–49 (1988). https://doi.org/10.1007/BF00915155

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