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Role of elevated monocyte transforming growth factorβ (TGFβ) production in posttrauma immunosuppression

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Abstract

We previously reported that increased production of prostaglandin E2 by monocytes is a pivotal mechanism in posttrauma immunopathology. Here we characterize monocyte levels of transforming growth factorβ and examine the effects of elevated transforming growth factorβ on prostaglandin E2 release by patients' monocytes. Trauma patients' and normals' monocyte supernates (± stimulation with muramyl dipeptide) were acid treated and assayed for transforming growth factorβ using the mink lung-cell bioassay. Alternatively, human transforming growth factorβ was added to patients' and normals' monocytes and prostaglandin E2 production assayed. Significantly elevated transforming growth factorβ levels (median=181.7 pmol/106 monocytes) were detected in immunosuppressed patients' monocytes but not immuno-competent trauma patients' (median=32.0 pM) or normals' (median=20.4 pM) monocytes. Adding transforming growth factorβ to monocytes resulted in a significant elevation of prostaglandin E2 levels. Elevated monocyte transforming growth factorβ levels in trauma patients could be both suppressing T-lymphocyte functions and maintaining elevated monocyte prostaglandin E2 synthesis.

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Authors and Affiliations

  1. Department of Surgery, University of Massachusetts Medical Center, 01655, Worcester, Massachusetts

    Carol L. Miller-Graziano, Gyongyi Szabo, Katherine Griffey, Bela Mehta, Karen Kodys & Donna Catalano

  2. Department of Molecular Genetics and Microbiology, University of Massachusetts Medical Center, 01655, Worcester, Massachusetts

    Carol L. Miller-Graziano & Gyongyi Szabo

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  1. Carol L. Miller-Graziano
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  2. Gyongyi Szabo
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  3. Katherine Griffey
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  5. Karen Kodys
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  6. Donna Catalano
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Miller-Graziano, C.L., Szabo, G., Griffey, K. et al. Role of elevated monocyte transforming growth factorβ (TGFβ) production in posttrauma immunosuppression. J Clin Immunol 11, 95–102 (1991). https://doi.org/10.1007/BF00917745

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  • DOI: https://doi.org/10.1007/BF00917745

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