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β-Adrenergic receptor activity of cerebral microvessels is reduced in aged rats

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Abstract

The effect of age on beta-(β) adrenergic receptor number (Bmax) and adenylate cyclase (AC) activity was determined in microvessels isolated from male F-344 rats at 3, 18, and 24 months of age. Scatchard analysis of [125I]iodocyanopindolol (ICYP) binding indicated reduced Bmax (fmol/mg) of microvessels isolated from 24 month old rats (27.2±4.9) compared with 3 month old (50.4±5.2) and 18 month old rats (p<0.01) (61.4±7.6). The basal AC activity (pmol cAMP/mg) in 24 month old rats (32.0 ±6.7) and in 18 month old rats (30.4±2.1) were significantly reduced compared to the basal activity in the young (50.1±4.2). The net isoproterenol or NaF stimulated AC activity in 24 month old rats (zero and 15.6±8.5 respectively) was also reduced compared to young rats (10.1±3.9 and 166.0±21.2 respectively). It is concluded that aging is associated with reduced isoproterenol stimulated AC activity of cerebral microvessels. This reduction is the product of reduced β-adrenergic receptor number and reduced activity of AC in aged rat cerebral microvessels.

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Authors and Affiliations

  1. Tucson DVA Medical Center and Department of Medicine, University of Arizona College of Medicine, 1821 East Elm Street, Tucson, Arizona

    Arshag D. Mooradian

  2. GRECC, Gainsville DVA Medical Center, Department of Pharmacology, University of Florida, 32608, Gainsville, Florida

    Philip J. Scarpace

Authors
  1. Arshag D. Mooradian
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  2. Philip J. Scarpace
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Mooradian, A.D., Scarpace, P.J. β-Adrenergic receptor activity of cerebral microvessels is reduced in aged rats. Neurochem Res 16, 447–451 (1991). https://doi.org/10.1007/BF00965565

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