Skip to main content
SpringerLink
Log in

HPLC determination of caffeine and theophylline inPaullinia cupana Kunth (Guarana) and Cola spp. samples

HPLC-Bestimmung von Coffein und Theophyllin inPaullinia cupana- und in Cola spp.-Proben

  • Original Papers
  • Published:
Zeitschrift für Lebensmittel-Untersuchung und Forschung Aims and scope Submit manuscript

Zusammenfassung

Die Anwendung einer RP-HPLC für die mengenmässige Bestimmung von Coffein und Theophyllin in Guarana-Proben (Droge aus den Samen desPaullinia cupana-Baumgewächses Kunth, Familie:Sapindaceae) des Amazonas-Gebiet und in Cola spp.-Proben wird beschrieben. Die analytische Methode ist einfach, nicht zeitaufwendig und erfordert nur ein Mindestmaß an analytischen Operationen für die Proben-Zubereitung. Der Vergleich von 5 verschiedenen Extraktionstechniken für Coffein wird ebenfalls erörtert. Außerdem wurde der Gehalt von Coffein und Theophyllin in fünf verschiedenen brasilianischen Guarana-Proben, zwei Proben diätetischer Erzeugnisse mit Guarana-Gehalt, zwei Cola-Extraktund drei Cola-Samen-Proben bestimmt.

Summary

A reversed-phase high-performance liquid-chromatographic method for the determination of caffeine and theophylline in commercial guarana samples (drug obtained from the seeds ofPaulinia cupana Kunth,Sapindaceae of the Amazon Region) and inCola spp. samples is described and discussed.

The methodology developed is simple and rapid with a minimum of samples preparation required. A comparision of five different techniques for the extraction of caffeine and theophylline is discussed. Furthermore the quantitative determination of caffeine and theophylline in five samples of Brasilian guarana, in two samples of dietetic products containing guarana, in two samples ofCola extract and in three ofCola seed powder are reported.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  1. Mich H, Haberler F (1954) Monatsh Chem 85:779

    Google Scholar 

  2. Bertrand G, de Berredo Carneiro P (1932) Compt Rend 194:26

    Google Scholar 

  3. Weevers T (1926) Verslag Akad Wetenschappen Amsterdam 35:301

    Google Scholar 

  4. Weevers T (1926) Proc R Acad Amsterdam 29:1048

    Google Scholar 

  5. Weevers T (1930) Arch Neerland Sci III B 5:11

    Google Scholar 

  6. Nierestein M (1922) J Chem Soc 121:23

    Google Scholar 

  7. Michaluk A (1954) Acta Polon Pharm 2:263

    Google Scholar 

  8. Descartes de Garcia Paula R, Iachan A (1956) Bol Inst Nacl Tecnol 7:43

    Google Scholar 

  9. Descartes de Garcia Paula R, Iachan A (1958) Anais Farm Quim Sâo Paulo 9:11

    Google Scholar 

  10. Whistler RL, Zimmerman Stein J (1951) J Am Chem Soc 73:4187

    Google Scholar 

  11. Whistler RL, Durso DP (1951) J Am Chem Soc 73:4189

    Google Scholar 

  12. Urgate T (1921) J Pharm Chim 24:378

    Google Scholar 

  13. Oehrli A (1927) Pharm Acta Helv 2:155

    Google Scholar 

  14. Urgate T (1930) Chemia 7:155

    Google Scholar 

  15. Wasicky R, Akisue G (1966) Rev Fac Farm Bioquim Univ Sâo Paulo 4:85

    Google Scholar 

  16. Markovic D (1947) Farm Glasnik 3:1

    Google Scholar 

  17. Goldman S (1959) Fedr Eng Quim 11:15

    Google Scholar 

  18. Maravalhas N (1965) Inst Nacl Pesquisas Amazonia Publ Quim 5:11

    Google Scholar 

  19. Stanislas E, Fouraste I (1976) J Pharm Belg 31:599

    Google Scholar 

  20. Miko JV, Mike, SV (1926) Pharm. Zentralhalle 67:193

    Google Scholar 

  21. Bertrand G, de Berredo de Carneiro P (1931) Bull Soc Chim 49:1093

    Google Scholar 

  22. Bertrand G, de Berredo de Carneiro P (1932) Bull Sci Pharmacol 39:273

    Google Scholar 

  23. Helon JH (1953) Anais Arm Quim Sâo Paulo 6:7

    Google Scholar 

  24. de Berredo de Carneiro P (1932) Ann Fals 25:419

    Google Scholar 

  25. Tocchini RP, Okada M, Angelucci E (1977) Colet Inst Tecnol Aliment 8:391

    Google Scholar 

  26. Okada M, Tocchini RP, Mori E, Angelucci E (1977) Colet Inst Tecnol Aliment 8:519

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Istituto di Chimica Farmaceutica e Tossicologica, Laboratorio di R. M. N. e Spettroscopie applicate alla Tossicologia, Facoltà di Farmacia, Università di Torino, cso Raffaello, 31, 10125, Torino, Italy

    Flavio Belliardo, Aldo Martelli & Maria Grazia Valle

Authors
  1. Flavio Belliardo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Aldo Martelli
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Maria Grazia Valle
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Belliardo, F., Martelli, A. & Valle, M.G. HPLC determination of caffeine and theophylline inPaullinia cupana Kunth (Guarana) and Cola spp. samples. Z Lebensm Unters Forch 180, 398–401 (1985). https://doi.org/10.1007/BF01027774

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01027774

Keywords

Search

Navigation