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Bile acid stimulation of colonic adenylate cyclase and secretion in the rabbit

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Abstract

In vitro studies have suggested that the cyclic AMP system may be the mediator of bile acid-induced colonic secretion. The aims of our experiments were to quantify thein vivo effect of various doses of deoxycholic acid (DCA) on adenylate cyclase activity (AC) and net secretion in the rabbit colon. AC increased significantly (P<0.01) with each increasing concentration of DCA; at 8 mM the activity was 126±6 pmoles cAMP/mg protein/min, or 4 times the control. Phosphodiesterase activity (PDE) was affected and significantly decreased (P<0.01) only by 8 mM DCA. The volume of luminal fluid increased significantly (P<0.01) as a bicarbonate-rich solution with 4, 6, and 8 mM DCA in graded fashion. In conclusion, stimulation of a colonic mucosal cAMP system is strongly implicated as mediating DCA-induced colonic secretion.

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Authors and Affiliations

  1. Division of Gastroenterology, Cedars-Sinai Medical Center, 8720 Beverly Boulevard, 90048, Los Angeles, California

    Dean R. Conley MD, Martin J. Coyne MD, George G. Bonorris, Ane Chung PhD & Leslie J. Schoenfield MD, PhD

Authors
  1. Dean R. Conley MD
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  2. Martin J. Coyne MD
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  3. George G. Bonorris
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  4. Ane Chung PhD
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  5. Leslie J. Schoenfield MD, PhD
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Supported in part by NIH grant AM 15631.

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Conley, D.R., Coyne, M.J., Bonorris, G.G. et al. Bile acid stimulation of colonic adenylate cyclase and secretion in the rabbit. Digest Dis Sci 21, 453–458 (1976). https://doi.org/10.1007/BF01072128

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