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Nitrate and vitamin C from fruits and vegetables: Impact of intake variations on nitrate and nitrite excretions of humans

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Abstract

The objective of the study was to determine nitrate + nitrite excretions of human subjects fed variable amounts of nitrates and nitrites and vitamin C from fruits and vegetables. During four, randomly-arranged experimental periods of seven days each, the 12 apparently healthy, adult human subjects consumed laboratory controlled, constant, diets which were systematically varied in kinds of fruits and vegetable to provide the four following variations: 414 mg nitrate + nitrite and 23 mg vitamin C, 412 mg nitrate + nitrite and 177 mg vitamin C, 23 mg nitrate + nitrite and 39 mg vitamin C, and 21 mg nitrate + nitrite and 193 mg vitamin C per subject per day, respectively. Subjects made complete collections of urine and stools throughout the study. Regardless of type of experimental diet fed, no nitrates and nitrites were detected in the feces. Urinary excretion of nitrate + nitrite was significantly greater at the higher levels of nitrate + nitrite intake than at the lower intake levels. Increased intake of vitamin C at either level of nitrate + nitrite intake resulted in apparent decreased urinary excretions of nitrite + nitrate.

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References

  • Anderson K, Valceschini G, Kies C (1982) Nitrate and nitrite excretion of humans as influenced by addition of spinach to free-choice diets. Nutr Rep Int 27: 77–85.

    Google Scholar 

  • Bartholomew B, Hill MJ (1984) The pharmacology of dietary nitrate and the origin of urinary nitrate. Food Chem Toxicol 22: 789–795.

    PubMed  Google Scholar 

  • Carlson MP, Schneider NR (1986) Determination of nitrate in forages by using selective ion electrode: collaborative study. J Assoc Off Anal Chem 69: 196–198.

    PubMed  Google Scholar 

  • Comly HH (1945) Cyanosis in infants caused by nitrates in well water. JAMA 129: 112–116.

    Google Scholar 

  • Correa P, Cuello C, Fajardo LF, Haenszel W, Bolanos O, De Ramirez B (1983) Diet and gastric cancer; nutrition survey in a high-risk area. J Natl Cancer Inst 70: 673–678.

    PubMed  Google Scholar 

  • Cuello C, Correa P, Haenszel W, Gordillo G, Brown C, Archer M, Tannesbaum S (1976) Gastric cancer in Colombia 1. Cancer risk and suspect environmental agents. J Natl Cancer Inst 57: 1015–1020.

    PubMed  Google Scholar 

  • Dutt MC, Lim HY, Chen RK (1987) Nitrate consumption and the incidence of gastric cancer in Singapore. Food Chem Toxicol 25: 515–520.

    PubMed  Google Scholar 

  • Fine DH (1982) Nitrosamines in the general environment and food, in PN Magee (ed) Nitrosamines and Human Cancer. Harbor Press, Cold Spring Harbor, NY, pp. 199, 201.

    Google Scholar 

  • Fine DH, Ross R, Rounbehler DP, Silvergleid A, Song L (1977) Formation in vivo of volatile N-nitrosamines in man after ingestion of cooked bacon and spinach. Nature 265: 753–755.

    PubMed  Google Scholar 

  • Forman D (1987) Gastric cancer, diet and nitrate exposure. Br Med J 294: 528–529.

    Google Scholar 

  • Gray JE, Dugan LR (1975) Inhibition of N-nitrosamine formation in model food systems. J Food Sci 40: 981–984.

    Google Scholar 

  • Green LD, DeLuzuriaga K, Ruiz, Wagner, DA, Rand W, Istfan N, Yound VR, Tannenbaum SR (1981a) Nitrate biosynthesis in man. Proc Natl Acad Sci USA 78: 7764–7768.

    PubMed  Google Scholar 

  • Hartman PE (1982) Overview: nitrite load in the upper gastrointestinal tract — past, present, and future. in: PN Magee (ed.) Nitrosamines and Human Cancer. Harbor Press, Cold Spring Harbor, NY, p. 416.

    Google Scholar 

  • Kamm JJ, Dashman T, Newmark H, Mergens WJ (1977) Inhibition of aminenitrite hepatotoxicity by alph-tocopherol. Toxicol Appl Pharmacol 41: 575–583.

    PubMed  Google Scholar 

  • Kurzer MS, Calloway DH (1981) Nitrate and nitrogen balances in men. Am J Clin Nutr 34: 1305–1313.

    PubMed  Google Scholar 

  • Lee K, Greger JL, Consaul JR, Graham KL, Chinn BL (1986) Nitrate, nitrite balance and de novo synthesis of nitrate in humans consuming cured meats. Am J Clin Nutr 44: 1188–1194.

    Google Scholar 

  • Lu SH, Ohshima H, Fu HM, Tran Y, Li FM, Blettner M, Wahrendorf J, Bartsch H (1986) Urinary excretion of N-nitrosamino acids and nitrate by inhabitants of high- and low-risk areas for esophageal cancer in northern China: endogenous formation of nitrosoproline and its inhibition by vitamin C. Cancer Res 46: 1485–1491.

    PubMed  Google Scholar 

  • Mirvish SS (1983) The etiology of gastric cancer: intragastric nitrosamide formation and other theories. J Natl Cancer Inst 71: 630–647.

    Google Scholar 

  • Mirvish SS, Wallcane L, Eagen M, Shubik P (1972) Ascorbate-nitrite reaction: possible means of blocking the formation of carcinogenic N-nitroso compounds. Science 177: 65–67.

    PubMed  Google Scholar 

  • National Research Council/National Academy of Science (1981) The Health Effects of Nitrate, Nitrite and N-nitroso Compounds, I. National Academy of Science, Washington, DC.

    Google Scholar 

  • Oshima H, Bartsch H (1981) Quantitative estimation of endogenous nitrosation in humans by monitoring N-nitrosoproline excreted in the urine. Cancer Res 41: 3658–3662.

    PubMed  Google Scholar 

  • Saul RL, Kabir SH, Cohen Z, Bruce WR, Archer MC (1981) Reevaluation of nitrate and nitrite levels in the human intestine. Cancer Res 41: 2280–2283.

    PubMed  Google Scholar 

  • Sen NP, Donaldson B (1978) Improved colorimetric method for determining nitrate and nitrite in foods. J Assoc Off Anal Chem 61: 1389–1394.

    PubMed  Google Scholar 

  • Siciliano J, Krulick S, Heisler EG, Schwartz JH, White JW (1975) Nitrate and nitrite content of some fresh and processed market vegetables. J Agric Food Chem 23: 461–464.

    PubMed  Google Scholar 

  • Tannenbaum SR, Moran D, Rand W, Cuello C, Correa P (1979) Gastric cancer in Colombia IV. Nitrite and other ions in gastric contents of residents from a high-risk region. J Natl Cancer Inst 62: 9–12.

    PubMed  Google Scholar 

  • Tannenbaum SR, Mergens W (1980 Reaction of nitrite with vitamins C and E, in OA Levander, L Cheng (eds.) Micronutrient Interactions: Vitamins, Minerals and hazardous Elements. Ann NY Acad Sci 355: 267–275.

    PubMed  Google Scholar 

  • Wagner DA, Schultz DS, Deen WM, Young VR, Tannenbaum SR (1983a) Metabolic fate of an oral dose of15N-labeled nitrate in humans: Effect of diet supplementation with ascorbic acid. Cancer Res 43: 1921–1925.

    PubMed  Google Scholar 

  • Wagner DA, Shuker DE, Bilmazes C, Obiedzinski M, Baker I, Young VR, Tannenbaum SR (1985) Effect of vitamins C and E on endogenous synthesis of N-nitrosamino acids in humans: precursor-product studies with [15N] nitrate. Cancer Res 45: 6519–6522.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. The Texas Woman's University, Denton, Texas, USA

    C. Bednar

  2. Dept. of Nutritional Science and Dietetics, University of Nebraska-Lincoln, 68583-0807, Lincoln, NE, USA

    C. Kies

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  1. C. Bednar
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  2. C. Kies
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Published as University of Nebraska Agricultural Research Journal Series no. 9936.

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Bednar, C., Kies, C. Nitrate and vitamin C from fruits and vegetables: Impact of intake variations on nitrate and nitrite excretions of humans. Plant Food Hum Nutr 45, 71–80 (1994). https://doi.org/10.1007/BF01091231

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  • DOI: https://doi.org/10.1007/BF01091231

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