Summary
The amino acid sequence of the coat protein of watermelon mosaic virus 2 (WMV 2) was determined by a combination of peptide and nucleic acid sequencing. The coat protein of WMV 2 contained 281 amino acid residues including a single cysteine at position 132 and a blocked amino terminus. Comparison with the coat protein sequences of 20 strains of ten distinct potyviruses showed sequence homologies ranging from 43% to 69% except for the N strain of soybean mosaic virus (SMV-N), where the sequence homology with WMV 2 was 83%. This degree of homology and the location of sequence differences between WMV 2 and SMV-N is much closer to that observed between strains of the same virus than that found between distinct potyviruses. These data suggest that WMV 2 and SMV-N may be strains of the same virus.
Similar content being viewed by others
References
Abu-Samah N (1985) Relationship between watermelon mosaic virus 1 (WMV-1) and watermelon mosaic virus 2 (WMV-2) on the basis of nucleotide sequence homology. Pertanika 8: 281–287
Allison RF, Dougherty WG, Parks TD, Willis L, Johnston RE, Kelly ME, Armstrong FB (1985) Biochemical analysis of the capsid protein ene and capsid protein of tobacco etch virus: N-terminal amino acids are located on the virion's surface. Virology 147: 309–316
Allison RF, Sorenson JG, Kelly ME, Armstrong FB, Dougherty WG (1985) Sequence determination of the capsid protein gene and flanking regions of tobacco etch virus: evidence for the synthesis and processing of a polyprotein in potyvirus genome expression. Proc Natl Acad Sci USA 82: 3696–3972
Al-Musa A, Mansour A (1982) Some properties of a watermelon mosaic virus in Jordan. Plant Dis 66: 330–331
Bos L (1972) Soybean mosaic virus. CMI/AAB Descript Plant Vir 93
Davis RF (1986) Partial characterization of zucchini yellow mosaic virus isolated from squash in Turkey. Plant Dis 70: 735–738
Domier LL, Franklin KM, Shahbuddin M, Hellman GM, Overmeyer JH, Hiremath ST, Siaw MEE, Lomonossoff GP, Shaw JG, Rhoads RE (1986) The nucleotide sequence of tobacco vein mottling virus. Nucleic Acids Res 14: 5417–5430
Dougherty WG, Allison RF, Parks TD, Johnston RE, Feild MJ, Armstrong FB (1985) Nucleotide sequence at the 3′-terminus of pepper mottle virus genomic RNA: evidence for an alternative mode of capsid protein gene organization. Virology 146: 282–291
Eggenberger AL, Stark DM, Beachy RN (1989) The nucleotide sequence of a soybean mosaic virus coat protein coding region and its expression inEscherichia coli, Agrobacterium tumefaciens, and tobacco callus. J Gen Virol (in press)
Francki RIB (1983) Current problems in plant virus taxonomy. In: Matthews REF (ed) A critical appraisal of viral taxonomy. CRC Press, Boca Raton, pp 63–104
Gough KH, Azad AA, Hanna PJ, Shukla DD (1987) Nucleotide sequence of the capsid and nuclear inclusion protein genes from the Johnson grass strain of sugarcane mosaic virus RNA. J Gen Virol 68: 297–304
Gough KH, Shukla DD (1981) Coat protein of potyviruses. 1. Comparison of the four Australian strains of sugarcane mosaic virus. Virology 111: 455–462
Greber RS (1978) Watermelon mosaic virus 1 and 2 in Queensland cucurbit crops. Aust J Agric Res 29: 1235–1245
Gubler U, Hoffman BJ (1983) A simple and very efficient method for generating cDNA libraries. Gene 25: 263–269
Gunyuzlu PL, Tolin SA, Johnson JL (1987) The nucleotide sequence of the 3′-terminus of soybean mosaic virus. Phytopathology 77: 1766
Hollings M, Brunt AA (1981) Potyvirues. In: Kurstak E (ed) Handbook of plant virus infections: comparative diagnosis. Elsevier/North Holland, Amsterdam, pp 731–807
Kao F-T, Hartz JA, Law ML, Davidson JN (1982) Isolation and chromosomal localization of unique DNA sequences from a human genomic library. Proc Natl Acad Sci USA 79: 865–869
Lain S, Riechmann JL, Mendex E, Garcia JA (1988) Nucleotide sequence of the 3′terminal region of plum pox potyvirus RNA. Virus Res 10: 325–342
Lisa V, Dellavalle G (1981) Characterization of two potyviruses inCucurbita pepo. Phytopathol Z 100: 279–286
MacDonald RJ, Swift GH, Przybyla AE, Chirgwin JM (1987) Isolation of RNA using guanidine salts. Methods Enzymol 152: 219–227
Maiss E, Breyl E, Brisske A, Casper D (1988) Molecular cloning of cDNA complementary to the RNA genome of plum pox virus (PPV). J Phytopathol 122: 222–231
Maiss E, Timpe U, Brisske A, Jelkmann W, Casper R, Himmler G, Mattanovich D, Katinger HWD (1989) The complete nucleotide sequence of plum pox virus (PPV). J Gen Virol (in press)
Maniatis T, Fritsch EF, Sambrook J (1983) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, New York
Matthews REF (1982) Classification and nomenclature of viruses. Intervirology 17: 1–199
McKern NM, O'Donnell IJ, Stewart DJ, Clark BL (1985) Primary structure of pilin protein fromBacteroides nodosus strain 216: comparison with the corresponding protein from strain 198. J Gen Microbiol 131: 1–6
Miyada CG, Wallace RB (1987) Oligonucleotide hybridization techniques. Methods Enzymol 154: 94–107
Moghal SM, Francki RIB (1976) Towards a system for the identification and classification of potyviruses. I. Serology and amino acid composition of six distinct viruses. Virology 73: 350–362
Purcifull DE, Alderz WC, Simone GW, Hiebert E, Christie SR (1984) Serological relationships and partial characterization of zucchini yellow mosaic virus isolated from squash in Florida. Plant Dis 68: 230–233
Purcifull D, Edwardson E, Hiebert E, Gonsalves D (1984) Papaya ringspot virus. CMI/AAB Descript Plant Vir 292
Purcifull DE, Hiebert E (1979) Serological distinction of watermelon mosaic virus. Phytopathology 69: 112–116
Purcifull D, Hiebert E, Edwardson J (1984) Watermelon mosaic virus 2. CMI/AAB Descript Plant Vir 293
Ravelonandro M, Varveri C, Delbos R, Dunez J (1988) Nucleotide sequence of the capsid protein gene of plum pox potyvirus. J Gen Virol 69: 1509–1516
Rosner A, Raccah B (1988) Nucleotide sequences of the capsid protein gene of potato virus Y (PVY). Virus Genes 1: 255–260
Russo M, Martelli GP, Vovlas C, Ragozzini A (1979) Comparative studies on Mediterranean isolates of watermelon mosaic virus. Phytopathol Medit 18: 94–101
Sanger F, Coulson AR, Barrell BG, Smith AJH, Roe BA (1980) Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. J Mol Biol 143: 161–178
Schmelzer K (1966) Nachweis des spezifischen Wassermelonen-Mosaik-Virus in Kuba. Naturwissenschaften 53: 619
Shukla DD, Gough KH, Ward CW (1987) Coat protein of potyviruses 3. Comparison of amino acid sequence of the coat proteins of four Australian strains of sugarcane mosaic virus. Arch Virol 96: 59–74
Shukla DD, Inglis AS, McKern NM, Gough KH (1986) Coat protein of potyviruses. 2. Amino acid sequence of the coat protein of potato virus Y. Virology 152: 118–125
Shukla DD, Jilka J, Tosic M, Ford RE (1989) A novel approach to the serology of potyviruses involving affinity purified polyclonal antibodies directed towards virus-specific Ntermini of coat proteins. J Gen Virol 70: 13–23
Shukla DD, McKern NM, Ward CW (1988) Coat protein of potyviruses. 5. Symptomatology, serology and coat protein sequences of three strains of passionfruit woodiness virus. Arch Virol 102: 221–232
Shukla DD, McKern NM, Gough KH, Tracy SL, Letho SG (1988) Differentiation of potyviruses and their strains by high-performance liquid chromatographic peptide profiling of coat proteins. J Gen Virol 69: 493–502
Shukla DD, Strike PM, Tracy SL, Gough KH, Ward CW (1988) The N and C termini of the coat proteins of potyviruses are surface-located and the N terminus contains the major virus-specific epitopes. J Gen Virol 69: 1497–1508
Shukla DD, Thomas JE, McKern NM, Tracy SL, Ward CW (1988) Coat protein of potyviruses. 4. Comparison of biological properties, serological relationships and coat protein amino acid sequences of four strains of potato virus Y. Arch Virol 102: 207–219
Shukla DD, Tosic M, Jilka J, Ford RE, Toler RW, Langham MAC (1989) Taxonomy of potyviruses infecting maize, sorghum and sugarcane in Australia and the United States as determined by reactivities of polyclonal antibodies directed towards virus-specific Ntermini of coat proteins. Phytopathology 79: 223–229
Shukla DD, Ward CW (1988) Amino acid sequence homology of coat proteins as a basic for identification and classification of the potyvirus group. J Gen Virol 69: 2703–2710
Shukla DD, Ward CW (1989) Structure of potyvirus coat proteins and its application in the taxonomy of the potyvirus group. Adv Virus Res 36 (in press)
Takahashi T, Uyeda I, Oshima K, Shikata E (1988) Molecular cloning and nucleotide sequence of a region coding a coat protein of bean yellow mosaic virus CS strain genome. Abstract of Papers, 5th Int Congr Plant Pathol, Kyoto, Japan, p 78
Vovlas C, Hiebert E, Russo M (1981) Zucchini yellow fleck virus, a new potyvirus of zucchini squash. Phytopathol Medit 20: 123–128
Yamamoto T, Ishii M, Katsube T (1982) Serological grouping of watermelon mosaic virus isolates. Ann Phytopathol Soc Japan 48: 613–619
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yu, M.H., Frenkel, M.J., McKern, N.M. et al. Coat protein of Potyviruses. Archives of Virology 105, 55–64 (1989). https://doi.org/10.1007/BF01311116
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01311116