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Postmortem estimation of age at death based on aspartic acid racemization in dentin: Its applicability for root dentin

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Summary

The extent of aspartic acid racemization in total dentin and in dentin protein fractions from theroots of third molars was determined. In several cases coronal dentin was also investigated. The results of other authors, according to which the racemization of aspartic acid in root dentin apparently proceeds differently than in coronal dentin, could be confirmed. Consequently, the data published so far on age determination based on the extent of aspartic acid racemization in coronal dentin and the “entire dentin of longitudinal sections” cannot be applied to root dentin. In total root dentin and the acid soluble protein of root dentin, a close relationship was observed between the extent of aspartic acid racemization and age. Accordingly, estimation of age at death based on aspartic acid racemization in dentin is also possible for root dentin, apparently with good results. This is important particularly in those cases where a large portion of the coronal dentin is absent, for instance following dental treatment. In the investigation of root dentin, regression equations specific for root dentin must be employed in the estimation of age at death. Corresponding equations for third molars were calculated.

Zusammenfassung

Der Razemisierungsgrad von Asparaginsäure wurde in Gesamtdentin sowie in Dentinproteinfraktionen aus Zahnwurzeln dritter Molaren bestimmt, in einigen Fällen wurde zusätzlich Kronendentin mituntersucht. Die Ergebnisse anderer Autoren, nach denen die Razemisierung von Asparaginsäure in Zahnwurzeldentin offenbar anders verläuft als in Kronendentin, konnten bestätigt werden. Damit können die bislang zur Lebensaltersbestimmung aufgrund des Razemisierungsgrades von Asparaginsäure in Dentin veröffentlichten Daten für Kronendentin und “longitudinale Dentinsegmente” nicht ohne weiteres auf Wurzeldentin übertragen werden. Für Gesamtwurzeldentin und das säurelösliche Wurzeldentinprotein ergab sich eine enge Beziehung zwischen dem Razemisierungsgrad von Asparaginsäure dem Lebensalter. Eine Lebensaltersbestimmung aufgrund des Razemisierungsgrades von Asparaginsäure ist danach auch an Wurzeldentin mit offenbar guten Ergebnissen möglich. Dies ist vor allem in Fällen von Bedeutung, in denen beispielsweise nach zahnärztlichen Maßnahmen ein Großteil des Kronendentins fehlt. Wird Wurzeldentin untersucht, müssen speziell für Wurzeldentin etablierte Regressionsgleichungen für eine Lebensaltersbestimmung herangezogen werden. Entsprechende Daten für dritte Molaren wurden erarbeitet.

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Ritz, S., Schütz, H.W. & Peper, C. Postmortem estimation of age at death based on aspartic acid racemization in dentin: Its applicability for root dentin. Int J Leg Med 105, 289–293 (1993). https://doi.org/10.1007/BF01370387

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  • DOI: https://doi.org/10.1007/BF01370387

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