Abstract
The purpose of this study was to characterize the effects of aging on gastric acid secretion and on serum and antral concentrations of gastrin in rats. Young and old Fischer 344 rats were prepared with gastric fistulas. Twenty-four hours after surgery, graded doses of human synthetic gastrin-17 (SHG-17) (2, 5, 10, 20, and 40 μg/kg) were given intravenously in random order. Gastric secretions were collected for gastric acid measurement before and at 15-min intervals after each dose of gastrin. In a separate study, blood was collected and the stomachs were removed for antral gastrin extraction from fed young and old rats. Serum and antral gastrin was measured by radioimmunoassay. The basal and gastrin-stimulated acid secretions were significantly decreased in aged rats compared to the young rats. The basal acid output was 0.4±0.2 μeq/15 min in the aged rats and 1.5±0.5 μeq/15 min in the young. The maximal acid output stimulated by gastrin was 11.1±1.8 μeq/15 min in the aged rats and 24.2±2.8 μeq/15 min in the young. Both serum and antral concentrations of gastrin were significantly decreased in aged rats. Serum gastrin concentration was 114.8±7.4 pg/ml in the aged rats and 192.0±14.4 pg/ml in the young. Antral gastrin concentration was 3.9±0.5 μg/g tissue in the aged rats, which was significantly less than the concentration in the young (6.5±0.4 μg/g tissue). Antral gastrin content did not change with aging. Gastric acid secretion in aged rats is significantly decreased compared to the young in both the basal condition and in response to fixed doses of exogenous gastrin. Diminished concentrations of circulating gastrin may well be responsible, at least in part, for the diminished acid secretion in the aged rats.
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References
Schuster MM: Disorders of the aging GI system. Hosp Prac Sept:95–103, 1976
Keyrilainen O: Nature of gastric hypersecretion of acid in patients with duodenal ulcer. Ann Clin Res 29:1–54, 1980
Baron JH: The clinical use of gastric function test. Scand J Gastroenterol Suppl 6:9–46, 1970
Grossman MI, Kirsner JB, Gillespie IE: Basal and histalog-stimulated gastric secretion in control subjects and in patients with peptic ulcer or gastric cancer. Gastroenterology 45:14–26, 1963
Burhol PG, Myren J: Gastritis and gastric secretion.In The Physiology of Gastric Secretion. LS Semb, J Myren (eds). Baltimore, Williams & Wilkins, 1968, pp 626–632
Miyoshi A, Ohe K, Inagawa T, Inoue M, Uraki S, Tatsugami M, Noguchi A, Onda M, Yokoya H, Shirakawa T: A statistical study on the age distribution of gastric secretion in patients with peptic ulcer. Hiroshima J Med Sci 29:21–28, 1980
Portis SA, King JC: The gastrointestinal tract in the aged. JAMA 148:1073–1079, 1952
Polland WS: Histamine test meals. An analysis of nine hundred and eighty-eight consecutive test. Arch Intern Med 51:903–919, 1933
Baron JH: Studies of basal and peak acid output with an augmented histamine test. Gut 4:136–144, 1963
Siurala M, Isokoski M, Varis K, Kekki M: Prevalence of gastritis in a rural population. Bioptic study of subjects selected at random. Scand J Gastroenterol 3:211–223, 1968
Andrews GR, Haneman B, Arnold BJ, Booth JC, Taylor K: Atrophic gastritis in the aged. Aust Ann Med 16:230–235, 1967
Kimura K: Chronological transition of the fundic-pyloric border determined by stepwise biopsy of the lesser and greater curvatures of the stomach. Gastroenterology 63:584–592, 1972
Strickland RG, Mackay IR: A reappraisal of the nature and significance of chronic atrophic gastritis. Am J Dig Dis 18:426–440, 1973
Varis K, Ihamaki T, Harkonen M, Samloff IM, Siurala M: Gastric morphology, function, and immunology in first-degree relative of probands with pernicious anemia and controls. Scand J Gastroenterol 14:129–139, 1979
Villako K, Tamm A, Savisaar E, Ruttas M: Prevalence of antral and fundic gastritis in a randomly selected group of an Estonian rural population. Scand J Gastroenterol 11:817–822, 1976
Kekki M, Samloff IM, Ihamaki T, Varis K, Siurala M: Ageand sex-related behaviour of gastric acid secretion at the population level. Scand J Gastroenterol 17:737–743, 1982
Trudeau WL, McGuigan JE: Serum gastrin levels in patients with peptic ulcer disease. Gastroenterology 59:6–12, 1970
Blair EL, Greenwell JR, Grund ER, Reed JD, Sanders DJ: Gastrin response to meals of different composition in normal subjects. Gut 16:766–773, 1975
Lane A, Ivy AC, Ivy EK: Response of the chronic gastric fistula rat to histamine. Am J Physiol 190:221–228, 1957
Bollman JL: A cage which limits the activity of rats. J Lab Clin Med 33:1348, 1948
Jackson BM, Reeder DD, Thompson JC: Dynamic characteristics of gastrin release. Am J Surg 123:137–142, 1972
Walford RL: When is a mouse “old”? J Immunol 177:352–353, 1976
Holland JM: Mammalian Models for Research in Aging. National Academy Press, Washington, D.C., 1981, pp 45–47
Chesky JA, Rockstein M: Life span characteristics in the male Fischer rat. Exp Aging Res 2:399–407, 1976
Archimandritis A, Alegakis G, Theodoropoulos G, Kalos A, Drivas G, Melissinos K: Serum gastrin concentrations in healthy males and females of various ages. Acta Hepato-Gastroenterol 26:58–63, 1979
Track NS, Watters LM, Gauldie J: Motilin, human pancreatic polypeptide (HPP) and gastrin plasma concentrations in fasting subjects. Clin Biochem 12:109–117, 1979
Weiner I, Khalil T, Thompson JC, Rayford PL: Gastrin.In Gastrointestinal Endocrinology. JC Thompson, GH Greeley Jr, PL Rayford, CM Townsend Jr (eds). McGraw-Hill, New York, 1987, pp 194–212
Lehy T, Gres L, Ferreira de Castro E: Quantitation of gastrin and somatostatin cell population in the antral mucosa of the rat. Cell Tissue Res 198:325–333, 1979
Singh P, Rae-Venter B, Townsend CM Jr, Khalil T, Thompson JC: Gastrin receptors in normal and malignant gastrointestinal mucosa: Age-associated changes. Am J Physiol 249:G761-G769, 1985
Johnson LR: Regulation of the mucosal gastrin receptor (41626). Proc Soc Exp Biol Med 173:167–175, 1983
Khalil T, Fujimura M, Greeley GH Jr, Townsend CM Jr, Thompson JC: Effects of aging on serum gastrin and antral gastrin content in Sprague-Dawley rats. Gastroenterology 88:1445, 1985(abstract)
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Supported by grants from the National Institutes of Health (RO1 DK 15241, PO1 DK 35608, RCDA CA 00854, CA 38651) and a grant from the American Cancer Society (PDT-220).
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Khalil, T., Singh, P., Fujimura, M. et al. Effect of aging on gastric acid secretion, serum gastrin, and antral gastrin content in rats. Digest Dis Sci 33, 1544–1548 (1988). https://doi.org/10.1007/BF01535944
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DOI: https://doi.org/10.1007/BF01535944