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Polyethylene glycol-induced mammalian cell hybridization: Effect of polyethylene glycol molecular weight and concentration

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Somatic Cell Genetics

Abstract

The effects of polyethylene glycol (PEG) molecular weight and concentration on mammalian cell hybridization were studied. The peak hybridization-inducing activity with all grades of PEG from 400–6000 was found to occur in the concentration range of 50–55%. However, changes in concentration were seen to have different quantitative effects with different grades of PEG. For monolayer fusions, PEG 1000 at 50% seems to be the optimal combination of PEG molecular weight and concentration, in terms of both efficiency of hybridization and relative insensitivity to dilution effects.

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Authors and Affiliations

  1. Division of Human Genetics, Children's Hospital Medical Center, 02115, Boston, Massachusetts

    Richard L. Davidson, Kathleen A. O'Malley & Thomas B. Wheeler

  2. Department of Microbiology and Molecular Genetics, Harvard Medical School, 02115, Boston, Massachusetts

    Richard L. Davidson

Authors
  1. Richard L. Davidson
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  2. Kathleen A. O'Malley
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  3. Thomas B. Wheeler
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Davidson, R.L., O'Malley, K.A. & Wheeler, T.B. Polyethylene glycol-induced mammalian cell hybridization: Effect of polyethylene glycol molecular weight and concentration. Somat Cell Mol Genet 2, 271–280 (1976). https://doi.org/10.1007/BF01538965

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  • DOI: https://doi.org/10.1007/BF01538965

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