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Binding of osteopontin to the osteoclast integrin αvβ3

  • Session VII: Pathogenesis: Bone Cells, Hormones And Growth Factors
  • Published:
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Abstract

Occupancy of the chicken osteoclast αvβ3 integrin stimulates immediate cell signals. Peptides from osteopontin containing Arg-Gly-Asp and peptides from the osteopontin and bone sialoprotein sequences containing Arg-Gly-Asp stimulated immediate reductions in osteoclast cytosolic Ca2+. The changes in cytosolic Ca2+ required the Arg-Gly-Asp sequence, and were blocked by LM609, a monoclonal antibody to the αvβ3 integrin. Osteoclast stimulation by the proteins through the integrin did not require immobilization since soluble peptides produced changes in cytosolic Ca2+ and inhibited osteoclast binding to bone particles and bone resorption. The decrease in cytosolic Ca2+ stimulated by osteopontin and related peptides was due to activation of a plasma membrane Ca2+-ATPase. Thus, the data suggest that ligand binding to the osteoclast αvβ3 integrin results in a reduction in cytosolic Ca2+ which participates in regulation of osteoclast function.

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References

  1. Gothlin G, Ericsson JLE. Clin Orthop Rel Res 1976;120:201–28.

    Google Scholar 

  2. Holtrop ME, King GJ. Clin Orthop Rel Res 1977;123:177–96.

    Google Scholar 

  3. Zambonin-Zallone A, Teti A, Grano M, Rubinacci A, Abbadini M, Gaboli M, Marchisio PC. Exp Cell Res 1989;182:645–52.

    Google Scholar 

  4. Marchisio PC, Cirillo D, Naldini L, Primavera V, Teti A, Zambone-Zallone A. J Cell Biol 1984;99:1696–705.

    Google Scholar 

  5. Marchisio PC, Cirillo D, Teti A, Zambone-Zallone A, Tarone G. Exp Cell Res 1987;169:202–14.

    Google Scholar 

  6. Horton MA. ISI Atlas Sci Immunol 1988;1:35–43.

    Google Scholar 

  7. Davies J, Warwick J, Totty N, Robin P, Helfrich M, Horton M. J Cell Biol 1989;109:1817–26.

    Google Scholar 

  8. Reinholt FP, Hultenby K, Oldberg A, Heinegard D. Proc Natl Acad Sci 1990;87:4473–5.

    Google Scholar 

  9. Oldberg A, Franzen A, Heinegard D. Proc Natl Acad Sci USA 1986;93:8819–23.

    Google Scholar 

  10. Oldberg A, Franzen A, Heinegard D, Pierschbacher M, Ruoslahti. J Biol Chem 1988;263:19433–5.

    Google Scholar 

  11. Cheresh DA, Spiro RC. J Biol Chem 1987;262:17703–11.

    Google Scholar 

  12. Cheresh DA, Harper JR. J Biol Chem 1987;262:1434–7.

    Google Scholar 

  13. Alvarez JI, Teitelbaum SL, Blair HC, Greenfield EM, Athanasou NA, Ross FP. Endocrinology 1991;128.

  14. Miyauchi A, Hruska KA, Greenfield EM, Duncan R, Alvarez J, Barattolo R, Colucci S, et al. J Cell Biol 1990;111:2543–52.

    Google Scholar 

  15. Teti A, Blair HC, Schlesinger P, Grano M, Zambonin-Zallone A, Kahn AJ, et al. J Clin Invest 1989;84:773–80.

    Google Scholar 

  16. Hynes RO, Marcantonio EE, Stepp MA, Urry LA, Yee GH. J Cell Biol 1989;109:409–20.

    Google Scholar 

  17. Malgaroli A, Fesce R, Meldolesi J. J Biol Chem 1990;265:3005–8.

    Google Scholar 

  18. Takemura H, Hughes AR, Thastrup O, Putney JW Jr. J Biol Chem 1989;264:12266–71.

    Google Scholar 

  19. van Os CH. Biochim Biophys Acta 1986;906:195–222.

    Google Scholar 

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Miyauchi, A., Alvarez, J., Greenfield, E.M. et al. Binding of osteopontin to the osteoclast integrin αvβ3 . Osteoporosis Int 3 (Suppl 1), 132–135 (1993). https://doi.org/10.1007/BF01621887

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  • DOI: https://doi.org/10.1007/BF01621887

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