Skip to main content
Log in

Synergistic effect of acute hypoxia on flow-induced release of ATP from cultured endothelial cells

  • Research Articles
  • Published:
Experientia Aims and scope Submit manuscript

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Abstract

Human umbilical vein endothelial cells (HUVECs) in primary cultures were perfused under normoxic or hypoxic conditions. These cells were stimulated twice for 3 min by increased flow (from 0.5 to 3.0 ml/min). Under hypoxic conditions the basal release of ATP was the same as under normoxic conditions, but during increased flow the release was greater (0.58±0.07>0.32±0.04 pmoles/ml/106 cells (+78%), for the first period of stimulation; 0.39±0.05>0.22±0.03 pmoles/ml/106 cells (+79%) for the second period). Further experiments with sequential increments in flow rate showed that under both normoxic and hypoxic conditions, a positive correlation existed between ATP release and the rate of flow but there was always more ATP released under hypoxic conditions regardless of the flow rate.

HUVECs in secondary culture (second passage) were similarly stimulated. No differences were observed between normoxic and hypoxic conditions. In both cases, the quantity of ATP released during high flow (0.050±0.004 pmoles/ml/106 cells) was significantly smaller than the quantity of ATP released during low flow (0.09±0.01 pmoles/ml/106 cells).

To conclude, since hypoxia alone did not affect ATP release, there appears to be a synergistic relationship between increased shear stress and hypoxia in the stimulation of ATP release from HUVECs. Moreover, the release of ATP under these conditions seems to be a property of highly differentiated endothelial cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Burnstock, G., Ann. N.Y. Acad. Sci.603 (1990) 31.

    Article  CAS  PubMed  Google Scholar 

  2. Burnstock, G., Trend. Pharmac. Sci.9 (1988) 116.

    Article  CAS  Google Scholar 

  3. Ralevic, V., Milner, P., Kirkpatrick, K. A., and Burnstock, G., Experientia48 (1992) 31.

    Article  CAS  PubMed  Google Scholar 

  4. Domer, F. R., Alexander, B., Milner, P., Bodin, P., and Burnstock, G., Brain Res.630 (1993) 88.

    Article  CAS  PubMed  Google Scholar 

  5. Hasséssian, H., Bodin, P., and Burstock, G., Br. J. Pharmac.109 (1993) 466.

    Article  Google Scholar 

  6. Milner, P., Ralevic, V., Hopwood, A. M., Fehér, E., Lincoln, J., Kirkpatrick, K., and Burnstock, G., Experientia45 (1989) 121.

    Article  CAS  PubMed  Google Scholar 

  7. Burnstock, G., and Kennedy, C., Gen. Pharmac.16 (1985) 433.

    Article  CAS  Google Scholar 

  8. Ralevic, V., and Burnstock, G., Neural-endothelial Interactions in the Control of Local Vascular Tone. Medical Intelligence Unit. RG Landes Company, Medical Publishers, Austin Texas, 1993.

    Google Scholar 

  9. Hopwood, A. M., Lincoln, J., Kirkpatrick, K. A., and Burnstock, G., Eur. J. Pharmac.165 (1989) 323.

    Article  CAS  Google Scholar 

  10. Sedaa, K. O., Bjur, R. A. Shinozvka, K., and Westfall, D. P., J. Pharmac. exp. Ther.252 (1990) 1060.

    CAS  Google Scholar 

  11. Bodin, P., Bailey, D., and Burnstock, G., Br. J. Pharmac.103, (1991) 1203.

    Article  CAS  Google Scholar 

  12. Milner, P., Loesch, A., and Burnstock, G., Biochem. biophys. Res. Commun.170 (1990) 649.

    Article  CAS  PubMed  Google Scholar 

  13. Rubanyi, G. M., and Vanhoutte, P. M., J. Physiol.364 (1985) 45.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Rubanyi, G. M., Romero, J. C., and Vanhoutte, P. M., Am. J. Physiol.250 (1986) H1145.

    Article  CAS  PubMed  Google Scholar 

  15. Rakugi, H., Yoshikatsu, T., Nakamaru, M., Nagano, M., Highashimori, K., Mikami, H., Ogihara, T., and Suzuki, N., Biochem. biophys. Res. Commun.169 (1990) 973.

    Article  CAS  PubMed  Google Scholar 

  16. Milner, P., Bodin, P., Loesch, A., and Burnstock, G., J. vasc. Res.29 (1992) 420.

    Article  CAS  PubMed  Google Scholar 

  17. Bodin, P., Milner, P., Winter, R., and Burnstock, G., Proc. R. Soc. B.247 (1992) 131.

    Article  CAS  PubMed  Google Scholar 

  18. Paddle, B. M., and Burnstock, G., Blood Vessels11 (1974) 110.

    CAS  PubMed  Google Scholar 

  19. Jaffe, E. A., Nachman, R. L., Becker, C. G., and Minick, C. R., J. clin. Invest.52 (1973) 2745.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Kirkpatrick, K., and Burnstock, G., Eur. J. Pharmac.138 (1987) 207.

    Article  CAS  Google Scholar 

  21. Bradford, M. M., Analyt. Biochem.72 (1976) 248.

    Article  CAS  PubMed  Google Scholar 

  22. Milner, P., Kirkpatrick, K. A., Ralevic, V., Toothill, V., Pearson, J., and Burnstock, G., Proc. R. Soc. B.241 (1990) 245.

    Article  CAS  PubMed  Google Scholar 

  23. Furchgott, R. F., and Zawadzki, J. V., Nature288 (1980) 375.

    Article  Google Scholar 

  24. Palmer, M. J., Ferige, A. G., and Moncada, S., Nature327 (1987) 524.

    Article  CAS  PubMed  Google Scholar 

  25. Moncada, S., Palmer, R. M. J., and Higgs, E. A., in: Thrombosis and Haemostasis, p. 597. Eds. J. Verstraete, J. Vermylen and H. H. R. Lijnen. Leuven University Press, Leuven 1987.

    Google Scholar 

  26. Yanagisawa, M., Kurihara, H., Kimura, S., Tomobe, Y., Kobayashi, M., Mitsui, Y., Yazaki, Y., Goto K., and Masaki, T., Nature332 (1988) 411.

    Article  CAS  PubMed  Google Scholar 

  27. Clemens, M. G., and Forester, T., J. Physiol.312 (1980) 143.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bodin, P., Burnstock, G. Synergistic effect of acute hypoxia on flow-induced release of ATP from cultured endothelial cells. Experientia 51, 256–259 (1995). https://doi.org/10.1007/BF01931108

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01931108

Key words

Navigation