Abstract
There is known to be a significant correlation between the number of glucocorticoid receptors in tissues and their anti-inflammatory effect. In this work, the specific binding of glucocorticoids was studied in inflammatory fibroblasts. Human fibroblasts were obtained from the knee joint of a rheumatoid patient undergoing surgery; experimental fibroblasts were from rat granulomas. The same study was carried out in quiescent synovial fibroblasts from a healthy subject (post-traumatic amputation) and from rat subcutaneous conjunctive tissue.
Fibroblasts were obtained by explant cultures and subcultures in monolayers. The stimulation state of cells was evaluated by the amounts of PGE2 and PGF2α released into the culture media. Analysis of the proportions of steroid bound to whole cells showed evidence of specific glucocorticoid receptors in all fibroblasts. Their number was three times higher in cells from inflammatory tissues than from controls. This increased number of receptors in inflammatory cells could be the result of the action of one or more mediators that promote their biosynthesis.
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References
E.D. Harris,Pathogenesis of rheumatoid arthritis,in:W.N. Kelley, E.D. Harris, S. Ruddy, C.B. Sledge. Textbook of rheumatology, 896–927 (W.B. Saunders, Philadelphia, 1981).
J.M. Dayer, S.M. Krane, G.G. Russel andD.R. Robinson,Production of collagenase and prostaglandins by isolated adherent rheumatoid synovial cells, Proc. Nat. Acad. Sci.73, 945–949 (1976).
J.M. Dayer, I. Bread, L. Chess andM. Stephen,Participation of monocyte-macrophages and lymphocytes in the production of a factor that stimulates collagenase and prostaglandin release by rheumatoid synovial cells, J. Clin. Invest.64, 1386–1392 (1979).
F. Blotman, P. Poubelle, J. Chaintreuil, M. Damon, O. Flandre, A. Crastes de Paulet andL. Simon,Mononuclear phagocytes, prostanoids and rheumatoid arthritis, Int. J. Immunopharmac.4, 119–125 (1982).
R. Hashida, S. Kobasyashi, H. Shirota, K. Yoshimatsu, S. Ohsawa, H. Hori, S. Hattori andY. Nagai,Stimulation of prostaglandin synthesis in cultured rat synovial cells by a factor derived from polymorphonuclear leukocytes, Prostaglandins27, 697–709 (1984).
A.M. Nelson andD.L. Conn,Glucocorticoids in rheumatic disease, Mayo Clin. Proc.55, 758–769 (1980).
L. Aronow,Effects of glucocorticoids on fibroblasts,in:J.D. Baxter,G.G. Rouseau. Glucocorticoid hormone action, 327–340 (Springer Verlag, Berlin Heidelberg New York (1979).
Y. Koshihara, M. Yamagishi andS.I. Murota,Effect of salicylic acid on glucocorticoid receptor in cultured fibroblasts derived from rat carrageenin granuloma, Biochim. Biophys. Acta437, 221–228 (1976).
S. Murota, Y. Koshihara andS. Tsurufuji,Effects of cortisol and tetrahydrocortisol on the cloned fibroblast derived from rat carrageenin granuloma, Biochem. Pharmacol.25, 1107–1115 (1976).
I.P. Braidman, K. Collins, C. Jones, K. Moris andM.I.V. Jayson,Evidence for a steroid receptor in rheumatoid synovial tissue cells, Agents and Actions, 233–236 (1980).
M.V. Ropes, G.A. Bennet, S. Cobb, R.F. Jacox andR.A. Jessar,Revision of the diagnostic criteria for rheumatoid arthritis,Bull. Rheum. Dis. 9 175–197 (1958).
O. Steinbrocker, C.H. Traeger, R.C. Batterman,Therapeutic criteria in rheumatoid arthritis, J. Am. Med. Ass.140, 659 (1949).
O. Flandre, M. Damon andJ. Secchi,Etude des techniques de production des granulomes experimentaux in: etude experimentale des anti-inflammatoires, 29–37 (Doin,Paris,1966).
W. Castor, F.F. Fries andA. Arbor,Composition and function of human synovial connective cells measured in vitro, J. Lab. Clin. Med.57, 394–407 (1961).
F. Russo-Marie, M. Paing andD. Duval,Involvement of receptors in steroid-induced inhibition of prostaglandins secretion, J. Biol. Chem.254, 84498–8504 (1979).
M. Kalimi andS. Seifter,Glucocorticoid receptors in WI38 fibroblasts characterization and changes with population doubling in culture, Biochim. Boiphys. Acta583, 352–359 (1979).
P.F. Bruning, W.J. Meyer andC.J. Migeon,Glucocorticoid receptor in cultured human skin fibroblasts, J. Steroid Biochem.10, 587–593 (1979).
G. Scatchard,The attractions of proteins for small molecules and ions, Ann. N.Y. Acad. Sci.51, 660 (1949).
F. Dray, B. Charbonnel andJ. Maclouf,Radioimmunoassay of prostaglandins F 1,E 1 and E 2 in human plasma, J. Clin. Invest.5, 311–318 (1975).
M.K.M. McGuire, I.E. Meats andR.G.G. Russel,Cell to cell interactions in the rheumatoid joint, Agents and Actions10, 486–490 (1980).
J.M. Dayer, D.R. Robinson andS.M. Krane,Prostaglandins production by rheumatoid synovial cells: stimulation by a factor from human mononuclear cells, J. Exp. Med.145, 1399–1404 (1977).
D. Feldman, J.W. Funder andI.S. Edelman,Subcellular mechanisms in the action of adrenal steroids, Am. J. Med.53, 545–560 (1972).
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Damon, M., Rabier, M., Loubatiere, J. et al. Glucocorticoid receptors in fibroblasts from synovial tissue. Changes during the inflammatory process. Preliminary results. Agents and Actions 17, 478–483 (1986). https://doi.org/10.1007/BF01965517
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DOI: https://doi.org/10.1007/BF01965517