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Rat mast cells synthesize a nitric oxide like-factor which modulates the release of histamine

  • Histamine Release from Mast Cells and Basophils
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Abstract

Rat serosal mast cells were evaluated for their capacity to generate a nitric oxide-like factor by two bioassays: inhibition of platelet aggregation and stimulation of mast cell guanylate cyclase. Incubation of mast cells with human washed platelets, both treated with indomethacin, inhibited thrombin-induced platelet aggregation which was potentiated by superoxide dismutase and reversed by oxyhaemoglobin. When mast cells alone were stirred at 1000 rpm, a time dependent increase in the levels of their cGMP but not cAMP was observed. Preincubation of mast cells with NG-monomethyl-l-arginine significantly enhanced E. coli lipopolysaccharide-evoked histamine release. Our results show that mast cell histamine release can be modulated by an intrinsically generated nitric oxide-like factor.

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Authors and Affiliations

  1. Department of Preclinical and Clinical Pharmacology, Viale G.B. Morgagni 65, I-50134, Florence, Italy

    E. Masini, A. Pistelli & P. F. Mannaioni

  2. The William Harvey Research Institute, St. Bartholomew's Hospital Medical College, Charterhouse Square, EC1M 6BQ, London, UK

    D. Salvemini & J. R. Vane

Authors
  1. E. Masini
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  2. D. Salvemini
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  3. A. Pistelli
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  4. P. F. Mannaioni
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  5. J. R. Vane
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Masini, E., Salvemini, D., Pistelli, A. et al. Rat mast cells synthesize a nitric oxide like-factor which modulates the release of histamine. Agents and Actions 33, 61–63 (1991). https://doi.org/10.1007/BF01993127

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  • DOI: https://doi.org/10.1007/BF01993127

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