Abstract
Normal human blood neutrophils were studied for their capacity to synthesize and release interleukin-1 (IL-1) species after phagocytosis of triclinic monosodium urate (MSU) and calcium pyrophosphate dihydrate crystals (CPPD). MSU crystals were more potent inducers of IL-1 generation than CPPD or unopsonized zymosan. Microcrystal-stimulated neutrophils characteristically secreted most of the newly synthesized IL-1. Colchicine partly inhibited the secretion of IL-1 by neutrophils during phagocytosis of solid particles. However, colchicine selectively inhibited IL-1 synthesis induced by microcrystals. These results suggest that neutrophil-derived IL-1 may contribute to the pathogenesis of crystal-induced arthritis.
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References
P. E. Poubelle, R. De Médicis and P. H. Naccache,Monosodium urate and calcium pyrophosphate crystals differentially activate the excitation-response coupling sequence of human neutrophils. Biochem. Biophys. Res. Commun.149, 649–657 (1987).
G. W. Duff, E. Atkins and S. E. Malawista,The fever of gout: urate crystals activate endogenous pyrogen production from human and rabbit mononuclear phagocytes. Trans. Assoc. Am. Physicians.96, 234–245 (1983).
J.-M. Dayer, V. Evêquoz, C. Zavadil-Grob, M. D. Grynpas, P. Cheng, J. Schnyder, V. Trechsel and H. Fleisch,Effect of synthetic calcium pyrophosphate and hydroxyapatite crystals on the interaction of human blood mononuclear cells with chondrocytes, synovial cells, and fibroblasts. Arthritis Rheum.30, 1372–1381 (1987).
K. Goto, S. Nakamura, F. Goto and M. Yoshinaga,Production of a lymphocyte proliferation potentiating factor by purified polymorphonuclear leukocytes from mice and rabbits. Immunology.53, 683–692 (1984).
K. Tiku, M. L. Tiku and J. L. Skosey,Interleukin-1 production by human polymorphonuclear neutrophils. J. Immunol.136, 3677–3685 (1986).
A. Lindemann, D. Riedel, W. Oster, S. C. Meuer, D. Blohm, R. H. Mertelsmann and F. Herrmann,Granulocyte/ macrophage colony-stimulating factor induces interleukin 1 production by human polymorphonuclear neutrophils. J. Immunol.140, 837–839 (1988).
J. Grassi, Y. Frobert, P. Pradelles, F. Chercuitte, D. Gruaz, J.-M. Dayer and P. E. Poubelle,Production of monoclonal antibodies against interleukins 1α and 1β. Development of two enzyme immunometric assays (EIA) using acetylcholinesterase and application to biological media. J. Immunol. Meth.123, 193–210 (1989).
C. W. Denko and M. W. Whitehouse,Experimental inflammation induced by naturally occuring microcrystalline salts. J. Rheumatol.3, 54–62 (1986).
A. Boyum,Isolation of mononuclear cells and granulocytes from human blood. Scand. J. Clin. Lab. Invest.21, 77–89 (1968).
A. D. Woolf, A. M. E. Nouri, P. Woo, M. B. Richter, G. S. Panayi and T. Gibson,Interleukin-1 and the acute phase response in acute crystal synovitis. Br. J. Rheumatol.24 (suppl. 1), 203–209 (1985).
H. R. Schumacher,Pathogenesis of crystal-induced synovitis. Clin. Rheum. Dis.3, 105–131 (1977).
P. H. Naccache, M. Grimard, C. J. Roberge, C. Gilbert, A. Lussier, R. De Médicis and P. E. Poubelle,Crystal-induced neutrophil activation. I-Initiation and modulation of calcium mobilization and superoxide production by microcrystals. Arthritis Rheumatism34, 333–342 (1991).
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Roberge, C.J., Grassi, J., De Médicis, R. et al. Crystal-neutrophil interactions lead to interleukin-1 synthesis. Agents and Actions 34, 38–41 (1991). https://doi.org/10.1007/BF01993232
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DOI: https://doi.org/10.1007/BF01993232