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Efficient cell proliferation and predominant accumulation of ɛ-globin mRNA in human leukemic K562 cells which produce mostly Hb Gower 1

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Summary

Long-term cultures of K562(S) cells in 50–75 μM hemin allow the selection of ‘hemin-resistant’ K562 cells together with cells which proliferate efficiently while fully induced to express the human embryonic globin genes, as the hemoglobin Gower 1 (ζ2ε2) is the predominant hemoglobin produced. Our experiments demonstrate that these K562 cells accumulate mostly ɛ-globin mRNA (ɛ-globin mRNA/γ-globin mRNA=2.9) suggesting that the control of hemoglobin expression is at a pretranslational level.

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Authors and Affiliations

  1. Centro Studi Biochimici sul Morbo di Cooley, I-44100, Ferrara, Italy

    R. Gambari, F. Amelotti & R. Piva

  2. Dipartimento di Biopatologia Umana, Università degli Studi di Roma, Roma, Italy

    R. Gambari, F. Amelotti & R. Piva

Authors
  1. R. Gambari
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  2. F. Amelotti
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  3. R. Piva
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Additional information

We thank Dr Irene Bozzoni (Centro degli Acidi Nucleici, Università di Roma) for the pXCR7 probe. Address for reprint request: R.G. Centro Studi Biochimici sul Morbo di Cooley, Via Borsari 46, I-44100 Ferrara.

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Gambari, R., Amelotti, F. & Piva, R. Efficient cell proliferation and predominant accumulation of ɛ-globin mRNA in human leukemic K562 cells which produce mostly Hb Gower 1. Experientia 41, 673–675 (1985). https://doi.org/10.1007/BF02007714

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  • DOI: https://doi.org/10.1007/BF02007714

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