Summary
The frequencies and distribution of common fragile sites in normal Japanese individuals were studied using lymphocyte chromosome preparations from cultures under folate deprivation, 5-bromodeoxyuridine (BrdU) or aphidicolin treatment. Defining the 4% level of total breaks as a cut-off point, 13 folate-sensitive, 20 BrdU-induced and 8 aphidicolin-induced sites were identified in one or more individuals studied. After eliminating overlapping, 28 fragile sites were identified. Of these, 23 have been reported, while the other five have not been described. Of the latter, those at 4q34 and 6q22 were each found in only one individual. The site at 3q26.2 has not been reported but one at 3q27 has been identified. Excluding these three sites as inconclusive, there remain two sites hitherto undescribed: 1) a folate-sensitive site at 17q21, found in two of eight individuals studied, and 2) a BrdU-requiring site at 13q31, found in five of eight individuals.
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Kuwano, A., Sugio, Y., Murano, I. et al. Common fragile sites induced by folate deprivation, BrdU and aphidicolin: Their frequency and distribution in Japanese individuals. Jap J Human Genet 33, 355–364 (1988). https://doi.org/10.1007/BF02032866
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DOI: https://doi.org/10.1007/BF02032866
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