Abstract
The purpose of this study was to determine the effect of an acute exercise bout on rates of skeletal muscle protein turnover in the presence and absence of a sub-maximal dose of insulin-like growth factor-1 (IGF-1) in young and mature animals. Mice (C57BI/6) were sacrificed 12 hours following a low intensity treadmill run (12 m/min @ 8% grade for either 1 or 2.5 hours). Protein synthesis, degradation and net protein degradation were measured in an in vitro isolated soleus muscle preparation in the absence or presence of IGF-1 in control and acutely exercised animals. Protein synthesis rates were lower in mature compared to young animals, but rates of protein degradation were unchanged. In the soleus from the 4 month control mice, protein synthesis increased in response to a submaximal dose of IGF-1 (20nM), but this response was abolished in the 12 month old tissue. Following an acute exercise bout (2.5 hours), protein synthesis increased in the soleus of both age groups, however net protein degradation increased in mature but not young animals. Protein synthesis rates of acutely exercised soleus of mature animals were increased in response to 20nM IGF-1 up to levels seen in the younger animals post-exercise. Results show that rates of protein synthesis decline from adolescence to maturity and protein synthesis is elevated in young and mature animals during the recovery from an exercise bout of long duration. It was also concluded that the ability of IGF-1 to stimulate protein synthesis was abolished in mature muscle, however this response was restored following low intensity aerobic exercise.
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Willis, P.E., Parkhouse, W.S. The influence of insulin-like growth factor-1 on protein turnover rates following exercise in young and mature animals. AGE 17, 111–118 (1994). https://doi.org/10.1007/BF02435818
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DOI: https://doi.org/10.1007/BF02435818