Skip to main content
SpringerLink
Log in

Problems in the analysis of propofol in blood when protein precipitation is used in sample preparation

  • Originals
  • Published:
Chromatographia Aims and scope Submit manuscript

Summary

Propofol (2,6-diisopropylphenol) is one of a group of intravenously administered hypnotic agents enabling easy inducement of sleep. Increasingly wider application of this drug in anaesthesiology has led to increasing demands for its precise and reliable analysis in the physiological fluids and tissues of man. The identification of problems in the determination of propofol seems to be essential. This paper deals with difficulties of propofol analysis in blood by HPLC when protein precipitation is used in sample preparation. The data obtained show that introduction of large amounts of fluids (for example electrolytes or blood-substitutes) into the blood, for example during long surgical operations, can significantly influence the results from propofol analysis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. W. Roth, K. Beschke, R. Jauch, A. Zimmer, F. W. Koss, J. Chromatogr.222, 13 (1981).

    CAS  Google Scholar 

  2. R. D. McDowall, E. Doyle, G. S. Murkitt, V. S. Picot, J. Pharm. Biomed. Anal.7, 1087 (1986).

    Article  Google Scholar 

  3. T. Kirkpatrick, I. D. Cockshott, E. J. Douglas, W. S. Nimmo, Br. J. Anaesth.60, 146 (1988).

    CAS  Google Scholar 

  4. A. Shafer, V. A. Doze, S. L. Shafer, P. F. White, Anaesthesiology69, 348 (1988).

    CAS  Google Scholar 

  5. P. Dailland, I. D. Cockshott, J. D. Lirzin, P. Jacquinot, J. C. Jorrot, J. Devery, J. L. Harmey, C. Conseiller, Anesthesiology71, 827 (1989).

    CAS  Google Scholar 

  6. G. F. Plummer, J. chromatogr.421, 171 (1987).

    CAS  Google Scholar 

  7. I. Pavan, E. Buglione, M. Massiccio, C. Gregoretti, L. Burbi, M. Berardino, J. Chromatogr. Sci.30, 164 (1992).

    CAS  Google Scholar 

  8. A. L. Dawidowicz, A. Fijalkowska, J. Chromatogr. Sci.33, 377 (1995).

    CAS  Google Scholar 

  9. S. Angielski (Editor) Biochemia kliniczna i analityka (Clinical Biochemistry and Analysis), Panstwowy Zaklad Wydawnictw Lekarskich, Warszawa, 1990, Chapters 11 and 13.

    Google Scholar 

  10. R. Richterich, Chemia kliniczna (Clinical Chemistry), Panstwowy Zaklad Wydawnictw Lekarskich, Warszawa, 1971, Chapter B II.

    Google Scholar 

  11. H. K. Adam, E. J. Douglas, G. F. Plummer, M. B. Cosgrove, J. Chromatogr.223, 232 (1981).

    CAS  Google Scholar 

  12. R. Larsen, Anästhesie, Urban and Schwarzenberg, München, 1995, Chapter, 10.

    Google Scholar 

  13. P. L. Stetson, E. F. Domino, J. R. Sneyd, J. Chromatogr.620, 260 (1993).

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemical Physics and Physicochemical Separation Methods, Faculty of Chemistry, Maria Curie Sklodowska University, M. Curie Sklodowska Square 3, 20-031, Lublin, Poland

    A. L. Dawidowicz & E. Fornal

  2. Department of Anaesthesia and Intensive Therapy, University School of Medicine, Jaczewski Street 8, 20-090, Lublin, Poland

    A. Fijalkowska

Authors
  1. A. L. Dawidowicz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. Fornal
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Fijalkowska
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dawidowicz, A.L., Fornal, E. & Fijalkowska, A. Problems in the analysis of propofol in blood when protein precipitation is used in sample preparation. Chromatographia 47, 523–528 (1998). https://doi.org/10.1007/BF02467489

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02467489

Key Words

Search

Navigation